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学者姓名：吴少华
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Abstract ：
The study of pear dormancy mechanism is currently a hot topic in pear production and has significant economic significance for agricultural production. Pyrus pyrifolia. 'Mixue' are known to have high nutritional and commercial value. To better understand the molecular mechanisms of bud dormancy release, we selected the southern sand pear cultivar Pyrus pyrifolia. 'Mixue' as the test material. We explored the respiratory metabolism rate and changes in endogenous hormones of pear flower buds during the dormancy process. Meanwhile, RNA sequencing was used to obtain the gene expression profile of Pyrus pyrifolia. 'Mixue' flower buds, exploring the molecular mechanism of pear flower bud dormancy. The results showed that there were 697 differentially expressed genes (DEGs) in E01vs E02, 665 DEGs in E01 vs E03, 738 DEGs in E01 vs E04, 581 DEGs in E03 vs E02, 664 DEGs in E03 vs E04, 668 genes were differentially expressed in E04 vs E02. KEGG pathway analysis showed that these DEGs were predominantly mapped to Plant-pathogen interaction pathway and Circadian rhythm-plant pathways in the process of dormancy. Furthermore, the genes related to asparagine synthetase (encoding the formation of ADP), glutamate synthetase 1 (encoding NADH, the precursor of starch formation), glutamate dehydrogenase glutamine synthetase leaf isozyme, CCA1 gene enriched in the Circadian rhythm-plant pathway and the four genes of the CML (calmodulin like) family (CML38, CML27, CML25, CML23) are significantly different in the process of pear dormancy, which may play an important role in the regulation of pear bud dormancy. In a word, these results will also be of great help in the regulation of endodormancy to cope with climate change and improve fruit production.
Keyword ：
Differentially expressed genes Differentially expressed genes Endodormancy Endodormancy Pyrus pyrifolia. 'Mixue' Pyrus pyrifolia. 'Mixue' Transcriptomes Transcriptomes
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| GB/T 7714 | Wang, Huiquan , Ye, Qinghua , Liu, Chunying et al. Comparative transcriptomic analysis provides insight into the key regulatory pathways and differentially expressed genes in Pyrus pyrifolia. 'Mixue' [J]. | JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY , 2025 , 34 (2) : 547-560 . |
| MLA | Wang, Huiquan et al. "Comparative transcriptomic analysis provides insight into the key regulatory pathways and differentially expressed genes in Pyrus pyrifolia. 'Mixue'" . | JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 34 . 2 (2025) : 547-560 . |
| APA | Wang, Huiquan , Ye, Qinghua , Liu, Chunying , Shen, Yunyu , Jiang, Yang , Huang, Xueying et al. Comparative transcriptomic analysis provides insight into the key regulatory pathways and differentially expressed genes in Pyrus pyrifolia. 'Mixue' . | JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY , 2025 , 34 (2) , 547-560 . |
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Pear buds exhibit inherent dormancy, during which carbohydrates play a pivotal role in dormancy release and germination. In this study, Pyrus pyrifolia 'Cuiguan' was employed as the experimental material to investigate the molecular mechanisms underlying flower bud dormancy release. The results revealed that the dynamic balance between starch and soluble sugar is critical for promoting dormancy release and germination in P. pyrifolia 'Cuiguan' flower buds. Through transcriptomic and proteomic profiling, a total of 4035 differentially expressed genes (DEGs) and 1596 differentially expressed proteins (DEPs) were identified, which were predominantly associated with carbohydrate metabolism, particularly sugar metabolism pathways. Their changes were coordinately regulated at both transcriptional and translational levels. Key structural genes involved in maltose and sucrose biosynthesis, including BAM (LOC103949270), AAM (LOC125479337, LOC103940334, and LOC103941903), SPS (LOC125475683), and INV (LOC125478747), were significantly upregulated during the germination stage, facilitating flower bud sprouting. Integrated multi-omic analysis demonstrated that starch-sugar interconversion may govern dormancy release and sustained bud growth by modulating sugar metabolism-related genes and proteins. These findings provide novel insights into the molecular mechanisms of carbohydrate biosynthesis and associated protein regulation during dormancy release and development of P. pyrifolia 'Cuiguan' under natural conditions.
Keyword ：
bud dormancy bud dormancy carbohydrate carbohydrate gene gene P. pyrifolia 'Cuiguan' P. pyrifolia 'Cuiguan' protein protein
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| GB/T 7714 | Wang, Huiquan , Ding, Ling , Ye, Qinghua et al. Transcriptomic and Proteomic Analyses Provide Insight into Sugar Metabolism-Induced Dormancy Release of Flower Buds of Pyrus pyrifolia 'Cuiguan' [J]. | HORTICULTURAE , 2025 , 11 (7) . |
| MLA | Wang, Huiquan et al. "Transcriptomic and Proteomic Analyses Provide Insight into Sugar Metabolism-Induced Dormancy Release of Flower Buds of Pyrus pyrifolia 'Cuiguan'" . | HORTICULTURAE 11 . 7 (2025) . |
| APA | Wang, Huiquan , Ding, Ling , Ye, Qinghua , Huang, Xueying , Xu, Lei , Wu, Shaohua et al. Transcriptomic and Proteomic Analyses Provide Insight into Sugar Metabolism-Induced Dormancy Release of Flower Buds of Pyrus pyrifolia 'Cuiguan' . | HORTICULTURAE , 2025 , 11 (7) . |
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Methyleugenol, a bioactive compound in the phenylpropene family, undergoes its final and crucial biosynthetic transformation when eugenol O-methyltransferase (EOMT) converts eugenol into methyleugenol. While Melaleuca bracteata F. Muell essential oil is particularly rich in methyleugenol, it contains only trace amounts of its precursor, eugenol. This suggests that the EOMT enzyme in M. bracteata is highly efficient, although it has not yet been characterized. In this study, we isolated and identified an EOMT gene from M. bracteata, termed MbEOMT1, which is primarily expressed in the flowers and leaves and is inducible by methyl jasmonate (MeJA). Subcellular localization of MbEOMT1 in the cytoplasm was detected. Through transient overexpression experiments, we found that MbEOMT1 significantly elevates the concentration of methyleugenol in M. bracteata leaves. Conversely, silencing of MbEOMT1 via virus-induced gene silencing led to a marked reduction in methyleugenol levels. Our in vitro enzymatic assays further confirmed that MbEOMT1 specifically catalyzes the methylation of eugenol. Collectively, these findings establish that the MbEOMT1 gene is critical for methyleugenol biosynthesis in M. bracteata. This study enriches the understanding of phenylpropene biosynthesis and suggests that MbEOMT1 could serve as a valuable catalyst for generating bioactive compounds in the future.
Keyword ：
fragrance fragrance methylation methylation O-methyltransferases (OMTs) O-methyltransferases (OMTs) phenylpropene phenylpropene shikimate pathway shikimate pathway synthesis synthesis
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| GB/T 7714 | Yang, Chao , Lin, Yongsheng , Xiang, Xuwen et al. MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell [J]. | TREE PHYSIOLOGY , 2024 , 44 (4) . |
| MLA | Yang, Chao et al. "MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell" . | TREE PHYSIOLOGY 44 . 4 (2024) . |
| APA | Yang, Chao , Lin, Yongsheng , Xiang, Xuwen , Shao, Dandan , Qiu, Ziwen , Li, Yongyu et al. MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell . | TREE PHYSIOLOGY , 2024 , 44 (4) . |
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The study of pear dormancy mechanisms is currently a major research area in pear production and has high economic significance for agricultural production. We selected the southern sand pear cultivar Pyrus pyrifolia 'Huanghua' as the test material to study the pear dormancy process through microscopic observation of pear flower buds. The endodormancy period is abbreviated as D_bud. Similarly, the endodormancy release initiation period and the ecodormancy period are referred to as DB_bud and G_bud, respectively. Meanwhile, RNA sequencing was used to obtain the gene expression profile of Pyrus pyrifolia 'Huanghua' flower buds. The RNA sequencing results indicated that there were 224 differentially expressed genes (DEGs) in endodormancy (D) vs. endodormancy release initiation period (DB), while 975 were identified between endodormancy and ecodormancy (G). Finally, a total of 868 DEGs were found in the DB vs. G comparison. The expression levels of the genes Pbr009498.1 (LAX1-1), Pbr012348.1 (LAX1-2), Pbr021158.1 (GH35), and Pbr031621.1 (LAX2) encoding IAA were significantly higher during the DB_bud than during the D_bud. The expression level of Pbr025864.2 (IAA13) during the D_bud was significantly higher than the DB_bud and G_bud. The Pbr041942.1 (GID1B) gene also showed a significant increase during ecodormancy. Taken together, these results suggest that these genes, annotated as LAX1, GH35, LAX2, IAA13, and GID1C, are involved in endodormancy maintenance and in the transition from endodormancy to ecodormancy in Pyrus pyrifolia 'Huanghua'.
Keyword ：
differentially expressed genes differentially expressed genes endodormancy endodormancy Pyrus pyrifolia 'Huanghua' Pyrus pyrifolia 'Huanghua' transcriptomes transcriptomes
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| GB/T 7714 | Wang, Huiquan , Liu, Chunying , Ye, Qinghua et al. Comparative Analysis of Transcriptomes to Identify Genes during Bud Dormancy of Pyrus pyrifolia 'Huanghua' [J]. | HORTICULTURAE , 2024 , 10 (6) . |
| MLA | Wang, Huiquan et al. "Comparative Analysis of Transcriptomes to Identify Genes during Bud Dormancy of Pyrus pyrifolia 'Huanghua'" . | HORTICULTURAE 10 . 6 (2024) . |
| APA | Wang, Huiquan , Liu, Chunying , Ye, Qinghua , Shen, Yunyu , Wu, Shaohua , Lin, Lizhong . Comparative Analysis of Transcriptomes to Identify Genes during Bud Dormancy of Pyrus pyrifolia 'Huanghua' . | HORTICULTURAE , 2024 , 10 (6) . |
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Melaleuca bracteata F. Muell. is among the most significant aromatic plants belonging to the Myrtaceae family, and its essential oil has various biological activities. However, the key enzymes involved in essential oil biosynthesis and their expression patterns are not clearly understood. Appropriate reference genes are required for quantitative real-time polymerase chain reaction (qRT-PCR), sensitive for gene expression studies, to obtain reliable results. In this study, 14 candidate genes, namely ACT, 18S, & alpha;-TUB, fl-TUB, GAPDH, EF1-& alpha;, UBQ, UBC, CYP, EIF-4A, DNAJ, PP2A, TIP41, and UBX, were selected from the M. bracteata transcriptome datasets for analysis under abiotic stresses and hormonal stimuli. The stability of these genes was analyzed using three widely used software programs (geNorm, NormFinder, and BestKeeper). Their results were integrated into a compre-hensive ranking by geometric mean. According to the study results, TIP41 and CYP were the most stable genes for abiotic stresses, while TIP41 and UBQ were the most stable genes for hormonal stimulation. Further, TIP41 and CYP were the most stable reference genes across all tested samples, whereas ACT was the least stable. Based on the expression of two target genes (COMT1 and COMT2, which are involved in methyleugenol biosynthesis), the suitability of the selected reference genes for qRT-PCR normalization was further verified. The study results could offer a foundation for gene expression analysis and functional studies in M. bracteata and other Melaleuca species.
Keyword ：
Abiotic stresses Abiotic stresses Gene expression Gene expression Hormonal stimuli Hormonal stimuli Melaleuca bracteata F Melaleuca bracteata F Muell Muell qRT-PCR qRT-PCR Reference gene Reference gene
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| GB/T 7714 | Yang, Chao , Lin, Yongsheng , Qiu, Ziwen et al. Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli [J]. | SCIENTIA HORTICULTURAE , 2023 , 319 . |
| MLA | Yang, Chao et al. "Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli" . | SCIENTIA HORTICULTURAE 319 (2023) . |
| APA | Yang, Chao , Lin, Yongsheng , Qiu, Ziwen , Xiang, Xuwen , Shao, Dandan , Li, Yongyu et al. Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli . | SCIENTIA HORTICULTURAE , 2023 , 319 . |
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Quorum sensing (QS) is a cell-to-cell communication in bacteria that couples gene expression through the accumulation of signaling molecules, which finally induce the production of several virulence factors and modulate bacterial behaviors. Plants have evolved an array of quorum sensing inhibitors (QSIs) to inhibit the pathogens, of which aromatic compounds are widely recognized. The essential oil of Melaleuca bracteata was found to exhibit anti-quorum sensing activity, and its principal bioactive component, methyleugenol (ME), had been isolated in our previous study. Here, ME interfered effectively with the QS-regulated processes of toxin secretion in Chomobacterium violaceum ATCC31532, resulting in strong inhibition of QS genes, cviR, cviI, vioA-E, hmsHNR, lasA-B, pilE1-3, and hcnABC, leading to impaired virulence, including violacein production, biofilm biomass, and swarming motility. The accumulation of the signal molecule (N-hexanoyl-DL-homoserine lactone, C6-HSL) in C. violaceum declined upon treatment with ME, suggesting an inhibition effect on the C6-HSL production, and the ME was also capable of degrading the C6-HSL in vitro assay. Molecular docking technique and the consumption change of exogenous C6-HSL in C. violaceum CV026 revealed the anti-QS mechanism of ME consisted of inhibition of C6-HSL production, potentially via interaction with CviR and/or CviI protein. Collectively, the isolated ME, the principal active components of M. bracteata EO, exhibited a wide range of inhibition processes targeting C. violaceum QS system, which supports the potential anti-pathogenic use of M. bracteata EO and ME for treatment of pathogen contamination caused by bacterial pathogens.
Keyword ：
Chomobacterium violaceum ATCC31532 Chomobacterium violaceum ATCC31532 Melaleuca bracteata EO Melaleuca bracteata EO methyleugenol methyleugenol quorum sensing inhibitor (QSI) quorum sensing inhibitor (QSI) signal molecule (C6-HSL) signal molecule (C6-HSL) virulence factors virulence factors
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| GB/T 7714 | Wang, Wenting , Lin, Xiaojie , Yang, Huixiang et al. Anti-quorum sensing evaluation of methyleugenol, the principal bioactive component, from the Melaleuca bracteata leaf oil [J]. | FRONTIERS IN MICROBIOLOGY , 2022 , 13 . |
| MLA | Wang, Wenting et al. "Anti-quorum sensing evaluation of methyleugenol, the principal bioactive component, from the Melaleuca bracteata leaf oil" . | FRONTIERS IN MICROBIOLOGY 13 (2022) . |
| APA | Wang, Wenting , Lin, Xiaojie , Yang, Huixiang , Huang, Xiaoqin , Pan, Lei , Wu, Shaohua et al. Anti-quorum sensing evaluation of methyleugenol, the principal bioactive component, from the Melaleuca bracteata leaf oil . | FRONTIERS IN MICROBIOLOGY , 2022 , 13 . |
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【目的】分析砂梨花芽休眠进程中内源激素的含量及平衡关系对梨花芽休眠进程的调控作用，为研究南方短低温梨花芽休眠解除的调控机理奠定基础。【方法】以黄花梨和蜜雪梨2个砂梨品种花芽为试材，利用HPLC技术分析赤霉素（GA_3）、脱落酸（ABA）、3-吲哚乙酸（IAA）、玉米素（ZT）等4种内源激素含量以及GA_3/ABA、IAA/ABA、ZT/ABA、（IAA+GA_3）/ABA比值在2个砂梨品种花芽休眠进程中的变化。【结果】在砂梨花芽休眠进程中，GA_3和ABA的影响主要在内休眠解除启动期，并且GA_3促进了花芽内休眠解除启动，而ABA抑制了花芽休眠解除启动；IAA在休眠进程中的变化规律不明显，对于休眠解除的影响不显著；ZT在黄花梨中促进了内休眠的解除，而在蜜雪梨中则作用于内休眠解除的启动。GA_3/ABA和（IAA+GA_3）/ABA在砂梨花芽休眠进程中变化趋势基本相同，均表现出在内休眠阶段比值较小，并且变化趋势不明显，而内休眠解除启动时明显升高，并且在内休眠解除时稍低于内休眠解除启动期；IAA/ABA其对休眠进程的影响较小；ZT/ABA的比值在黄花梨花芽休眠解除启动期稍低于内休眠解除期，而在蜜雪梨中花芽休眠解除启动期会稍高于内休眠解除期。【结论】砂梨花芽休眠进程中GA_3和ABA两种內源激素起主要作用，ZT不能直接影响休眠进程，而且内源激素调控花芽的休眠进程更主要取决于各内源激素的平衡。不同品种之间內源激素变化不完全一致，但总的趋势相似，本研究为內源激素调控休眠的研究奠定了基础。
Keyword ：
ABA ABA GA_3 GA_3 休眠 休眠 内源激素 内源激素 砂梨 砂梨
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| GB/T 7714 | 王会全 , 李亮 , 李永裕 et al. 内源激素对砂梨花芽休眠进程的调控作用 [J]. | 福建农业学报 , 2022 , 37 (12) : 1571-1578 . |
| MLA | 王会全 et al. "内源激素对砂梨花芽休眠进程的调控作用" . | 福建农业学报 37 . 12 (2022) : 1571-1578 . |
| APA | 王会全 , 李亮 , 李永裕 , 吴少华 . 内源激素对砂梨花芽休眠进程的调控作用 . | 福建农业学报 , 2022 , 37 (12) , 1571-1578 . |
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【目的】探究砂梨miR159家族成员进化特性及其在梨花芽休眠进程中的作用。【方法】通过检索已构建的砂梨（黄花）miRNA文库，筛选鉴定出2个miR159成熟体和2个前体序列，分别为ppy-miR159a和ppy-miR159b、ppyMIR159a和ppy-MIR159b。从黄花梨中克隆得到ppy-miR159a和ppy-miR159b。通过生物信息学分析梨miR159家族。采用实时荧光定量测定梨两个miR159家族成员及其靶基因pyrus＿GLEAN＿10035941(GAMYB转录因子)在梨花芽休眠进程中以及单氰胺（HC）处理梨休眠花芽的变化。【结果】植物miR159家族成熟体进化树的结果显示在进化树的大分支中，miR159家族的碱基高度重合，并且大多数miR159家族都具有相同的序列，说明了黄花梨与其他物种在进化过程中高度保守。而在进化树中出现各种小分支，说明miR159家族在进化过程由于功能分化，其成熟序列也出现相应的碱基改变。两个miR159家族成员的前体均能形成环状结构，且成熟体都在3’臂。ppy-miR159a和ppy-miR159b的最小折叠自由能（ΔG）分别为-368.81 kJ·mol-1和-352.96 kJ·mol-1。进化树分析发现梨miR159的亲缘关系与苹果更为接近。梨miR159调控的潜在靶基因主要是MYB家族基因，它与GA的调控有关，另外两个miR159的其他潜在靶基因并不完全相同，还包括蛋白激酶、细胞色素等功能不同的蛋白基因。在梨花芽休眠进程中，梨miR159相对表达量在整个休眠期间呈现先下降后上升再下降的双峰动态变化，靶基因pyrus＿GLEAN＿10035941的表达趋势与miR159相反，呈现先上升后下降再上升的变化，且miR159和靶基因的表达量均在休眠解除时期出现显著变化；而单氰胺（HC）处理梨休眠花芽的定量结果显示，随着HC破眠处理时间的增加，miR159的表达量降低，靶基因pyrus＿GLEAN＿10035941的表达量升高，靶基因pyrus＿GLEAN＿10035941变化趋势与miR159相反。【结论】两个miR159家族成员的潜在靶基因主要是MYB家族基因。梨花芽可能通过下调miR159表达，促进GAMYB表达，提高GA含量，促进花芽冬季休眠的解除。另外靶基因pyrus＿GLEAN＿10035941和DELLA基因的表达模式类似。
Keyword ：
miR159 miR159 休眠 休眠 砂梨 砂梨 表达模式 表达模式 进化特性 进化特性
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| GB/T 7714 | 刘瑾航 , 沈炳荣 , 李宜轩 et al. 砂梨miR159家族进化特性及在冬季休眠的表达模式分析 [J]. | 果树学报 , 2022 , 39 (11) : 2007-2018 . |
| MLA | 刘瑾航 et al. "砂梨miR159家族进化特性及在冬季休眠的表达模式分析" . | 果树学报 39 . 11 (2022) : 2007-2018 . |
| APA | 刘瑾航 , 沈炳荣 , 李宜轩 , 梁沁 , 冯宇 , 林晓洁 et al. 砂梨miR159家族进化特性及在冬季休眠的表达模式分析 . | 果树学报 , 2022 , 39 (11) , 2007-2018 . |
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在前期构建的千层金（Melaleucabracteata）转录组数据库中鉴定并克隆得到2个丁香酚合成酶基因MbEGS1和MbEGS2，基因编码区（codingsequences,CDs）序列长度分别为963和972bp，分别编码320和323个氨基酸。序列比对和系统进化树分析表明，MbEGS1、Mb EGS2与仙女扇（Clarkia breweri）CbIGS1蛋白序列相似性最高，达到65.92%和63.89%。qRT-PCR分析表明，二者在千层金的花、叶片和茎中均有表达，其中在花中表达量最高，叶中次之，茎中最低，与不同组织中丁香酚含量的变化类似，基因表达量与丁香酚含量呈显著正相关；MbEGS1在千层金的根中有表达，且表达量高于茎段，但低于叶片，而MbEGS2在根中不表达。采用不同浓度MeJA处理千层金发现，MeJA能够诱导MbEGS1和MbEGS2表达和丁香酚合成，0.1 mmol·L-1 MeJA诱导效果最显著，其转录水平与丁香酚含量呈正相关。亚细胞定位结果表明，Mb EGS1主要定位于细胞质膜和细胞核中，Mb EGS2主要定位于细胞质膜中。在森林草莓中异位表达MbEGS1和MbEGS2的结果表明，过表达MbEGS1和MbEGS2促进了丁香酚含量的增加。以上结果表明，二者均参与了丁香酚的生物合成。
Keyword ：
丁香酚合成酶 丁香酚合成酶 功能鉴定 功能鉴定 千层金 千层金 基因克隆 基因克隆 表达分析 表达分析
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| GB/T 7714 | 邱子文 , 刘林敏 , 林永盛 et al. 千层金MbEGS基因的克隆与功能分析 [J]. | 园艺学报 , 2022 , 49 (08) : 1747-1760 . |
| MLA | 邱子文 et al. "千层金MbEGS基因的克隆与功能分析" . | 园艺学报 49 . 08 (2022) : 1747-1760 . |
| APA | 邱子文 , 刘林敏 , 林永盛 , 林晓洁 , 李永裕 , 吴少华 et al. 千层金MbEGS基因的克隆与功能分析 . | 园艺学报 , 2022 , 49 (08) , 1747-1760 . |
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Abstract ：
研究盐胁迫对千层金生理指标、精油含量以及成分的影响，以期为盐渍地种植千层金提供参考依据。以千层金扦插苗为试验材料，探究不同NaCl浓度(0、100、200、300、400、500 mmol/L)下千层金扦插苗光合作用、脯氨酸含量、丙二醛含量、POD酶活性、CAT酶活性以及精油含量和成分的影响。结果表明，盐胁迫环境下，千层金表现出较强的耐盐性以及抗氧化性。在100、200、300 mmol/L的NaCl处理下，其精油含量与对照组(0 mmol/L)相比分别增加22.34%、22.14%和57.83%。因此，适当的盐胁迫不会对千层金的生理指标产生影响，且能增加千层金叶片中的精油含量。
Keyword ：
千层金 千层金 生理指标 生理指标 盐胁迫 盐胁迫 精油 精油
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| GB/T 7714 | 林永盛 , 桑洁丽 , 邱子文 et al. 盐胁迫对千层金生理指标及精油含量和成分的影响 [J]. | 天然产物研究与开发 , 2022 , 34 (03) : 420-426 . |
| MLA | 林永盛 et al. "盐胁迫对千层金生理指标及精油含量和成分的影响" . | 天然产物研究与开发 34 . 03 (2022) : 420-426 . |
| APA | 林永盛 , 桑洁丽 , 邱子文 , 尹冠文 , 林晓洁 , 吴少华 et al. 盐胁迫对千层金生理指标及精油含量和成分的影响 . | 天然产物研究与开发 , 2022 , 34 (03) , 420-426 . |
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