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学者姓名:梁学武
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Abstract :
The objective of this study was to investigate the effects of composite alkali-stored spent Hypsizygus marmoreus substrate (SHMS) on carcass quality, rumen fermentation, and rumen microbial diversity in goats. Twenty-four 6-month-old Chuanzhong black goats with similar body weights (20 +/- 5 kg) were selected and randomly divided into four groups (n = 6 per group) and received four treatments: 0% (control group, CG); 20% (low-addition group, LG); 30% (moderate-addition group, MG); and 40% (high-addition group, HG) of SHMS-replaced silage corn and oat hay. The experiment lasted for 74 days (including a 14 d adaptation period and a 60 d treatment period). The results of this study showed that MG and HG significantly improved the marble score of goat meat (p < 0.05). The flesh color score significantly increased in each group (p < 0.05). The fat color scores significantly increased in LG and MG (p < 0.05). There were no significant effects on the pH value or shear force of the longissimus dorsi in each group (p > 0.05). The cooking loss in MG was higher than that in CG (p < 0.05). The histidine and tyrosine contents in each group of muscles significantly increased (p < 0.05), with no significant effect on fatty acids (p > 0.05). The rumen pH of MG significantly decreased (p < 0.05), while the total volatile fatty acids (TVFAs) and ammoniacal nitrogen (NH3-N) increased by 44.63% and 54.50%, respectively. The addition of the SHMS altered both the alpha and beta diversities of the rumen microbiota and significant differences in the composition and structure of the four microbial communities. The dominant bacterial phylum in each group were Firmicutes and Bacteroidetes, with Prevotella 1 as the dominant bacterial genus. Correlation analysis revealed that rumen bacteria are closely related to the animal carcass quality and rumen fermentation. In the PICRUSt prediction, 21 significantly different pathways were found, and the correlation network showed a positive correlation between the Prevotella 1 and 7 metabolic pathways, while the C5-branched dibasic acid metabolism was positively correlated with nine bacteria. In summary, feeding goats with an SHMS diet can improve the carcass quality, promote rumen fermentation, and alter the microbial structure. The research results can provide a scientific reference for the utilization of SHMS as feed in the goat industry.
Keyword :
alkali storage alkali storage carcass quality carcass quality fattening goat fattening goat rumen microorganism rumen microorganism spent mushroom substrate spent mushroom substrate
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| GB/T 7714 | Qiu, Shuiling , Li, Keyao , He, Xiangbo et al. The Effects of Composite Alkali-Stored Spent Hypsizygus marmoreus Substrate on Carcass Quality, Rumen Fermentation, and Rumen Microbial Diversity in Goats [J]. | ANIMALS , 2024 , 14 (1) . |
| MLA | Qiu, Shuiling et al. "The Effects of Composite Alkali-Stored Spent Hypsizygus marmoreus Substrate on Carcass Quality, Rumen Fermentation, and Rumen Microbial Diversity in Goats" . | ANIMALS 14 . 1 (2024) . |
| APA | Qiu, Shuiling , Li, Keyao , He, Xiangbo , Gu, Mingming , Jiang, Xinghui , Lu, Jianing et al. The Effects of Composite Alkali-Stored Spent Hypsizygus marmoreus Substrate on Carcass Quality, Rumen Fermentation, and Rumen Microbial Diversity in Goats . | ANIMALS , 2024 , 14 (1) . |
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本试验旨在研究饲粮中添加布拉迪酵母对安格斯犊牛生长性能、血清免疫指标及粪便微生物区系的影响。试验选取(152±10)日龄、体重相近的健康安格斯犊牛24头,随机分为3组,每组8头牛。对照组(CG组)饲喂基础饲粮,试验组分别在基础饲粮中添加100(LG组)和200 mg/kg布拉迪酵母(HG组)。预试期14 d,正试期28 d。结果表明:1) LG和HG组犊牛终末体重和平均日增重较CG组差异不显著(P>0.05)。2) LG组犊牛体高较CG组显著提高(P<0.05)。3)LG和HG组犊牛血清白细胞介素-6(IL-6)含量较CG组极显著下降(P<0.01),且HG组显著高于LG组(P<0.05);LG组犊牛血清肿瘤坏死因子-α(TNF-α)含量较CG组显著下降(P<0.05),且HG组极显著高于LG组(P<0.01)。4) LG组Shannon指数较CG组显著上升(P<0.05)。5)在门水平上,3组的优势菌门均为厚壁菌门和拟杆菌门。在科水平上,3组的优势菌科均为瘤胃球菌科、理研菌科、毛螺菌科、普雷沃氏菌科。LG组梭菌目_vadinBB60群的相对丰度显著高于CG组(P<0.05),而丹尼丝菌科的相对丰度显著低于CG组(P<0.05)。HG组丹尼丝菌科的相对丰度显著高于CG组(P<0.05),而梭菌目_vadinBB60群的相对丰度显著低于CG组(P<0.05)。在属水平上,3组的优势菌属均为瘤胃球菌科_UCG-005、理研菌科_RC9_肠道群、瘤胃球菌科_UCG-010、拟杆菌属、艾克曼菌属。LG和HG组dgA-11_gut_group的相对丰度显著低于CG组(P<0.05)。线性判别分析效应量(LEfSe)分析结果显示,LG和HG组芽孢杆菌科、芽孢杆菌属等的相对丰度增加。综上所述,饲粮中添加布拉迪酵母对安格斯犊牛的生长性能无显著影响,可提高犊牛体高,降低血清促炎细胞因子IL-6和TNF-α含量,增强机体免疫功能,提高芽孢杆菌属等有益菌的相对丰度,改善粪便微生物区系,促进犊牛健康。本试验中,饲粮中添加100 mg/kg布拉迪酵母的效果最佳。
Keyword :
免疫 免疫 布拉迪酵母 布拉迪酵母 微生物区系 微生物区系 犊牛 犊牛 生长性能 生长性能
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| GB/T 7714 | 薛帅帅 , 李亚飞 , 李可瑶 et al. 饲粮中添加布拉迪酵母对安格斯犊牛生长性能、血清免疫指标及粪便微生物区系的影响 [J]. | 动物营养学报 , 2022 , 34 (06) : 3743-3757 . |
| MLA | 薛帅帅 et al. "饲粮中添加布拉迪酵母对安格斯犊牛生长性能、血清免疫指标及粪便微生物区系的影响" . | 动物营养学报 34 . 06 (2022) : 3743-3757 . |
| APA | 薛帅帅 , 李亚飞 , 李可瑶 , 刘浩宇 , 黄彩云 , 郭娉婷 et al. 饲粮中添加布拉迪酵母对安格斯犊牛生长性能、血清免疫指标及粪便微生物区系的影响 . | 动物营养学报 , 2022 , 34 (06) , 3743-3757 . |
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为了研究灵芝多糖对断奶川中黑山羊羔羊肝脏免疫功能的影响,试验选取 40~50 日龄、体重14 kg左右的断奶川中黑山羊公羔 48 只,随机分为 4 组,即空白对照(CON)组、低剂量(LGLP)组、中剂量(MGLP)组和高剂量(HGLP)组,每组 12 只.试验期间分别向CON 组、LGLP 组、MGLP 组和HGLP组基础饲粮中添加0,200,400,800 mg/kg(以干物质计)的灵芝多糖,预试期 7 d,正试期 56 d.试验结束后,每组随机选择6 只川中黑山羊屠宰,分离肝脏用于肝脏免疫指标测定和转录组测序,并对差异表达基因进行GO 生物学进程及KEGG 代谢通路分析.结果表明:与CON组比,MGLP 组(添加400 mg/kg灵芝多糖)可以显著提高川中黑山羊肝脏白细胞介素 6(IL-6)、肿瘤坏死因子 α(TNF-α)和IL-1β水平(P<0.05).MGLP组免疫效果最好,因此选择MGLP 组、CON组的肝脏样本进行转录组测序分析.转录组测序分析结果表明,MGLP组与CON组差异表达基因显著富集到抗原加工和提呈、T细胞受体信号通路、趋化因子信号通路和裂原激活蛋白激酶(MAPK)信号通路 4 条免疫相关通路(P<0.05).4 条免疫相关通路共筛选到 19 个差异表达基因,CCL5、CⅡTA、LOC102183314 等11 个差异表达基因显著上调(P<0.05),DUSP1、MYC、LOC102177850 等 8 个差异表达基因显著下调(P<0.05).说明添加 400 mg/kg灵芝多糖可以显著改善断奶川中黑山羊羔羊肝脏免疫功能,并通过调节抗原加工和提呈、T细胞受体信号通路、趋化因子信号通路和MAPK信号通路途径相关基因的表达来调节羔羊肝脏免疫功能.
Keyword :
免疫 免疫 川中黑山羊 川中黑山羊 断奶 断奶 灵芝多糖 灵芝多糖 肝脏 肝脏 转录组 转录组
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| GB/T 7714 | 苗景 , 何祥波 , 方邵明 et al. 基于转录组测序的灵芝多糖对公羊羔肝脏免疫功能的影响 [J]. | 黑龙江畜牧兽医(下半月) , 2021 , (3) : 118-123,149 . |
| MLA | 苗景 et al. "基于转录组测序的灵芝多糖对公羊羔肝脏免疫功能的影响" . | 黑龙江畜牧兽医(下半月) 3 (2021) : 118-123,149 . |
| APA | 苗景 , 何祥波 , 方邵明 , 林仕欣 , 梁学武 . 基于转录组测序的灵芝多糖对公羊羔肝脏免疫功能的影响 . | 黑龙江畜牧兽医(下半月) , 2021 , (3) , 118-123,149 . |
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为了研究灵芝多糖对断奶川中黑山羊羔羊肝脏免疫功能的影响,试验选取40~50日龄、体重14 kg左右的断奶川中黑山羊公羔48只,随机分为4组,即空白对照(CON)组、低剂量(LGLP)组、中剂量(MGLP)组和高剂量(HGLP)组,每组12只。试验期间分别向CON组、LGLP组、MGLP组和HGLP组基础饲粮中添加0,200,400,800 mg/kg(以干物质计)的灵芝多糖,预试期7 d,正试期56 d。试验结束后,每组随机选择6只川中黑山羊屠宰,分离肝脏用于肝脏免疫指标测定和转录组测序,并对差异表达基因进行GO生物学进程及KEGG代谢通路分析。结果表明:与CON组比,MGLP组(添加400 mg/kg灵芝多糖)可以显著提高川中黑山羊肝脏白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和IL-1β水平(P<0.05)。MGLP组免疫效果最好,因此选择MGLP组、CON组的肝脏样本进行转录组测序分析。转录组测序分析结果表明,MGLP组与CON组差异表达基因显著富集到抗原加工和提呈、T细胞受体信号通路、趋化因子信号通路和裂原激活蛋白激酶(MAPK)信号通路4条免疫相关通路(P<0.05)。4条免疫相关通路共筛选到19个差异表达基因,CCL5、CⅡTA、LOC102183314等11个差异表达基因显著上调(P<0.05),DUSP1、MYC、LOC102177850等8个差异表达基因显著下调(P<0.05)。说明添加400 mg/kg灵芝多糖可以显著改善断奶川中黑山羊羔羊肝脏免疫功能,并通过调节抗原加工和提呈、T细胞受体信号通路、趋化因子信号通路和MAPK信号通路途径相关基因的表达来调节羔羊肝脏免疫功能。
Keyword :
免疫 免疫 川中黑山羊 川中黑山羊 断奶 断奶 灵芝多糖 灵芝多糖 肝脏 肝脏 转录组 转录组
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| GB/T 7714 | 苗景 , 何祥波 , 方邵明 et al. 基于转录组测序的灵芝多糖对公羊羔肝脏免疫功能的影响 [J]. | 黑龙江畜牧兽医 , 2021 , (06) : 118-123,149 . |
| MLA | 苗景 et al. "基于转录组测序的灵芝多糖对公羊羔肝脏免疫功能的影响" . | 黑龙江畜牧兽医 06 (2021) : 118-123,149 . |
| APA | 苗景 , 何祥波 , 方邵明 , 林仕欣 , 梁学武 . 基于转录组测序的灵芝多糖对公羊羔肝脏免疫功能的影响 . | 黑龙江畜牧兽医 , 2021 , (06) , 118-123,149 . |
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本发明提供了一种与中国荷斯坦奶牛血清胰岛素浓度相关的SNP分子标记,属于分子育种技术领域。本发明在对中国荷斯坦奶牛进行全基因组扫描后,在其中显著关联的SNP位点中发现一项未公开发表的SP位点,位于奶牛20号染色体DOCK2基因中,在其序列的第14406bp处发生A/G突变。利用全基因组关联分析(GWAS)与高通量测序技术,可以更高效的揭示奶牛胰岛素分泌的遗传机制。
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| GB/T 7714 | 甘乾福 , 李毅冉 , 梁学武 et al. 一种与中国荷斯坦奶牛血清胰岛激素浓度相关的SNP分子标记 : CN202011289913.1[P]. | 2020-11-18 . |
| MLA | 甘乾福 et al. "一种与中国荷斯坦奶牛血清胰岛激素浓度相关的SNP分子标记" : CN202011289913.1. | 2020-11-18 . |
| APA | 甘乾福 , 李毅冉 , 梁学武 , 刘庆华 . 一种与中国荷斯坦奶牛血清胰岛激素浓度相关的SNP分子标记 : CN202011289913.1. | 2020-11-18 . |
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本发明提供了一种与中国荷斯坦奶牛血清镁离子浓度相关的SNP分子标记,属于分子育种技术领域。本发明在对中国荷斯坦奶牛进行全基因组扫描后,在其中显著关联的SNP位点中发现一项未公开发表的SNP位点,位于奶牛3号染色体KCNA2基因附近,距离28874bp处发生T/C突变。利用全基因组关联分析(GWAS)与高通量测序技术,可以更高效的揭示奶牛血清中离子浓度变化的遗传机制。
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| GB/T 7714 | 甘乾福 , 李毅冉 , 梁学武 et al. 一种与中国荷斯坦奶牛血清镁离子浓度相关的SNP分子标记 : CN202011289914.6[P]. | 2020-11-18 . |
| MLA | 甘乾福 et al. "一种与中国荷斯坦奶牛血清镁离子浓度相关的SNP分子标记" : CN202011289914.6. | 2020-11-18 . |
| APA | 甘乾福 , 李毅冉 , 梁学武 , 刘庆华 . 一种与中国荷斯坦奶牛血清镁离子浓度相关的SNP分子标记 : CN202011289914.6. | 2020-11-18 . |
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To further understand the genetic structure that is associated with insulin (INS) and thyroid hormones (TH), including triiodothyronine (T-3) and thyroxine (T-4), in Chinese Holstein cows, we conducted a genome-wide association study (GWAS) of thyroid hormones and insulin in cows. We conducted GWAS analysis on 1217 Chinese Holstein cows raised in southern China and found 19 significant single nucleotide polymorphisms (SNPs) in this study: 10 SNPs were associated with INS, 5 SNPs were associated with T-3, and 4 SNPs were associated with T-4. In our study, the GWAS method was used for preliminary screening on related genes of traits, and due to insufficient relevant literature, a functional analysis of genes could only be based on human studies. We observed thatDGKBfromBos tauruschromosome (BTA)4 is strongly associated with insulin secretion. We found thatEXOC4gene was significantly correlated with T(3)and T(4)traits. Another significant SNP was located in theCYP7A1gene, which has been confirmed to be affected by thyroid hormones.
Keyword :
Dairy cattle Dairy cattle DGKB DGKB FarmCPU FarmCPU Hormone secretion Hormone secretion
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| GB/T 7714 | Gan, QianFu , Li, YiRan , Liu, QingHua et al. Genome-wide association studies for the concentrations of insulin, triiodothyronine, and thyroxine in Chinese Holstein cattle [J]. | TROPICAL ANIMAL HEALTH AND PRODUCTION , 2020 , 52 (4) : 1655-1660 . |
| MLA | Gan, QianFu et al. "Genome-wide association studies for the concentrations of insulin, triiodothyronine, and thyroxine in Chinese Holstein cattle" . | TROPICAL ANIMAL HEALTH AND PRODUCTION 52 . 4 (2020) : 1655-1660 . |
| APA | Gan, QianFu , Li, YiRan , Liu, QingHua , Lund, M. , Su, GuoSheng , Liang, XueWu . Genome-wide association studies for the concentrations of insulin, triiodothyronine, and thyroxine in Chinese Holstein cattle . | TROPICAL ANIMAL HEALTH AND PRODUCTION , 2020 , 52 (4) , 1655-1660 . |
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选取24只体重相近(20 kg±5 kg)的川中黑山羊,随机分为4组,每组6只.对照组育肥羊饲喂不添加碱贮真姬菇菌糠的日粮,试验Ⅰ、Ⅱ、Ⅲ组育肥羊分别饲喂添加20%、30%和40%碱贮真姬菇菌糠的日粮,预饲期14 d,正饲期60 d,研究碱贮真姬菇菌糠对育肥羊生产性能、屠宰性能及血液生化指标的影响.结果表明,在日粮中添加不同水平的碱贮真姬菇菌糠可以降低饲料成本,提高经济效益且对育肥羊的生产性能、屠宰性能及血液生化指标没有不良影响.试验Ⅰ、Ⅱ、Ⅲ组育肥羊的平均日采食量较对照组分别提高了12.28%、16.37%和5.85%(P<0.05);试验组育肥羊的屠宰性能较对照组有提高的趋势,但差异不显著;试验组育肥羊血液中谷胱甘肽过氧化物酶和超氧化物歧化酶的活性较对照组均有提高,但差异不显著;试验Ⅱ组育肥羊的瘤胃重量和瘤胃指数较对照组分别提高了22.00%和12.03%(P<0.05),总胆固醇浓度较对照组提高了28.95%(P<0.05).综合评定结果显示,添加30%碱贮真姬菇菌糠的效果最佳.
Keyword :
屠宰性能 屠宰性能 生产性能 生产性能 真姬菇菌糠 真姬菇菌糠 碱贮 碱贮 育肥羊 育肥羊 血液生化指标 血液生化指标
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| GB/T 7714 | 何祥波 , 李毅冉 , 苗景 et al. 碱贮真姬菇菌糠对育肥羊生产性能、屠宰性能及血液生化指标的影响 [J]. | 福建农林大学学报(自然科学版) , 2020 , 49 (03) : 354-359 . |
| MLA | 何祥波 et al. "碱贮真姬菇菌糠对育肥羊生产性能、屠宰性能及血液生化指标的影响" . | 福建农林大学学报(自然科学版) 49 . 03 (2020) : 354-359 . |
| APA | 何祥波 , 李毅冉 , 苗景 , 许锦聪 , 梁学武 , 甘乾福 . 碱贮真姬菇菌糠对育肥羊生产性能、屠宰性能及血液生化指标的影响 . | 福建农林大学学报(自然科学版) , 2020 , 49 (03) , 354-359 . |
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We aimed to identify QTL for serum electrolyte traits by performing a GWAS of calcium, chloride, sodium, potassium and magnesium ion concentrations in Chinese Holstein cattle. We detected five SNPs that had significant associations with calcium ion concentrations and identified GATA2 from Bos taurus chromosome (BTA)22 as having the highest significance. Among the genes with significant results, we speculate that TMEM123 might be related to calcium channel proteins according to the functions of the TMEM family.
Keyword :
Calcium ions Calcium ions Chloride ion Chloride ion Dairy cattle Dairy cattle FarmCPU FarmCPU GATA2 GATA2
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| GB/T 7714 | Gan, Q. F. , Li, Y. R. , Lund, M. et al. Genome-wide association study identifies loci linked to serum electrolyte traits in Chinese Holstein cattle [J]. | ANIMAL GENETICS , 2019 , 50 (6) : 744-748 . |
| MLA | Gan, Q. F. et al. "Genome-wide association study identifies loci linked to serum electrolyte traits in Chinese Holstein cattle" . | ANIMAL GENETICS 50 . 6 (2019) : 744-748 . |
| APA | Gan, Q. F. , Li, Y. R. , Lund, M. , Su, G. S. , Liang, X. W. . Genome-wide association study identifies loci linked to serum electrolyte traits in Chinese Holstein cattle . | ANIMAL GENETICS , 2019 , 50 (6) , 744-748 . |
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Abstract :
灵芝多糖(GLP)是灵芝中主要功能性活性成分,具有增强免疫力、抗氧化、抗炎、抗肿瘤、降血糖和降血脂等功效。采用大肠杆菌内毒素脂多糖(LPS)诱导小鼠乳腺炎,研究灵芝多糖对小鼠乳腺炎的保护作用。试验选取刚分娩的健康雌性小鼠75只,分为空白对照组、LPS组、LPS+2%GLP组、LPS+4%GLP组和LPS+8%GLP组,LPS+2%GLP组、LPS+4%GLP组、LPS+8%GLP组分别灌胃2%、4%、8%的灵芝多糖溶液,空白对照组和LPS组灌胃等量的生理盐水。注射LPS后,测量小鼠体温变化,24 h后取乳腺制作石蜡组织切片,并测定乳腺中的IL-1β、IL-6、TNF-α含量和MPO活性。结果表明,与空白对照组相比,LPS组小鼠体温变化显著,乳腺组织切片出现明显的中性细胞浸润现象,IL-1β、IL-6、TNF-α含量和MPO活性显著上升;LPS+2%GLP组、LPS+4%GLP组、LPS+8%GLP组与LPS组相比,小鼠体温变化有显著改善,18 h后LPS+4%GLP组和LPS+8%GLP组与LPS组差异显著(P<0.05);与LPS组相比,LPS+2%GLP组、LPS+4%GLP组、LPS+8%GLP组小鼠乳腺中性细胞浸润现象明显减轻,LPS+4%GLP组和LPS+8%GLP组的TNF-α、IL-1β含量显著低于LPS组(P<0.05);LPS+4%GLP组和LPS+8%GLP组中IL-6含量显著低于LPS组(P<0.05),LPS+2%GLP组与LPS组差异不显著(P>0.05)。上述结果表明,添加4%、8%灵芝多糖可以减轻由LPS引起的小鼠乳腺炎反应。
Keyword :
乳腺炎 乳腺炎 小鼠 小鼠 灵芝多糖 灵芝多糖 脂多糖 脂多糖
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| GB/T 7714 | 苗景 , 何祥波 , 许锦聪 et al. 灵芝多糖对脂多糖诱导的小鼠乳腺炎的保护作用 [J]. | 江苏农业科学 , 2019 , 47 (23) : 197-200 . |
| MLA | 苗景 et al. "灵芝多糖对脂多糖诱导的小鼠乳腺炎的保护作用" . | 江苏农业科学 47 . 23 (2019) : 197-200 . |
| APA | 苗景 , 何祥波 , 许锦聪 , 林仕欣 , 梁学武 . 灵芝多糖对脂多糖诱导的小鼠乳腺炎的保护作用 . | 江苏农业科学 , 2019 , 47 (23) , 197-200 . |
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