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学者姓名：周冰峰
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【目的】蜜蜂卵、幼虫和蛹的发育具有明显的狭温性特征，低温胁迫会导致发育停滞。本研究旨在研究西方蜜蜂Apis mellifera工蜂预蛹低温胁迫后恢复化蛹的分子机制。【方法】比较在最适温度35℃发育的西方蜜蜂工蜂蛹(CK)和预蛹经20℃低温胁迫24 h后恢复化蛹的蛹(T)的转录组测序数据，筛选CK vs T比较组的差异表达基因(differentially expressed genes, DEGs);将DEGs进行GO功能注释和KEGG通路富集；选取5个抗氧化相关的DEGs(SOD2,Tpx4,GstS1,Prdx6和Cu-Zn)进行RT-qPCR验证。【结果】CK vs T比较组检测到1 335个DEGs,其中853个DEGs上调，482个DEGs下调；GO富集结果显示，DEGs显著富集在细胞内核糖核蛋白复合物、 NADH脱氢酶活性和抗氧化活性等GO条目上。KEGG富集结果显示，DEGs显著富集在氧化磷酸化、核糖体以及蛋白酶体通路。抗氧化相关5个DEGs (SOD2,Tpx4,GstS1,Prdx6和Cu-Zn)的RT-qPCR结果均显示上调表达，与转录组测序结果趋势一致。【结论】经低温胁迫的西方蜜蜂预蛹，当恢复正常发育温度后，通过提高能量代谢、蛋白质的合成分解以及抗氧化相关基因的表达来实现重启发育并完成化蛹。本研究有助于深入了解狭温性昆虫在经历低温胁迫后的发育恢复机制。
Keyword ：
代谢 代谢 低温胁迫 低温胁迫 化蛹 化蛹 抗氧化酶 抗氧化酶 西方蜜蜂 西方蜜蜂 转录组 转录组
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| GB/T 7714 | 曹铭劼 , 许宏智 , 徐新建 et al. 西方蜜蜂预蛹低温胁迫后恢复化蛹的转录组学分析 [J]. | 昆虫学报 , 2025 , 68 (03) : 291-299 . |
| MLA | 曹铭劼 et al. "西方蜜蜂预蛹低温胁迫后恢复化蛹的转录组学分析" . | 昆虫学报 68 . 03 (2025) : 291-299 . |
| APA | 曹铭劼 , 许宏智 , 徐新建 , 周姝婧 , 朱晨煜 , 田园明月 et al. 西方蜜蜂预蛹低温胁迫后恢复化蛹的转录组学分析 . | 昆虫学报 , 2025 , 68 (03) , 291-299 . |
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Exposure to low temperatures during honeybee development has been shown to impede brain development and affect cognitive function in adult bees. On the other hand, neuronal damage due to oxidative stress has been reported in many cases. Hence, biochemical parameters related to oxidative stress in honeybee pupae brain were determined. The levels of GSH in the pupal brain decreased after 24 h and 48 h of exposure to low temperatures; there were also reduced activities of SOD and CAT enzymes following 48 h of low-temperature treatment compared to the control group. Furthermore, analysis of transcriptome data post-24 h and -48 h low-temperature stress revealed the suppression of the glutathione metabolism and peroxisome pathways in pupal brains. Additionally, expression pattern clustering analysis and KEGG enrichment showed that 10 differentially expressed genes with down-regulated expression trends post-low-temperature treatment were significantly enriched in the peroxisome pathway, including PEX10, highlighting their connection to peroxisome function. RT-qPCR validation was conducted on 11 core enriched genes in pathways identified via GSEA, and all these genes exhibited a downregulated expression pattern, confirming the inhibition of glutathione metabolism and peroxisome function under low-temperature stress. The present study showed that exposing honeybee pupae to low temperatures suppressed both the glutathione metabolism and peroxisome pathways, resulting in increased oxidative stress. This research enhances our understanding of how the pupal brain reacts to cold stress and illuminates the neural damage associated with low temperatures during honeybee capped brood development.
Keyword ：
brain development brain development cold stress cold stress honeybee honeybee newly pupated stage newly pupated stage oxidative stress oxidative stress
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| GB/T 7714 | Zhu, Xiangjie , Cao, Mingjie , Li, Chenyang et al. Biochemical and Transcriptomic Analysis Reveals Low Temperature-Driven Oxidative Stress in Pupal Apis mellifera Neural System [J]. | INSECTS , 2025 , 16 (3) . |
| MLA | Zhu, Xiangjie et al. "Biochemical and Transcriptomic Analysis Reveals Low Temperature-Driven Oxidative Stress in Pupal Apis mellifera Neural System" . | INSECTS 16 . 3 (2025) . |
| APA | Zhu, Xiangjie , Cao, Mingjie , Li, Chenyang , Zhu, Chenyu , Li, Han , Tian, Yuanmingyue et al. Biochemical and Transcriptomic Analysis Reveals Low Temperature-Driven Oxidative Stress in Pupal Apis mellifera Neural System . | INSECTS , 2025 , 16 (3) . |
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Thermal condition affects the development and growth of ectotherms. The stenothermic honeybee brood, particularly the prepupae, are sensitive to low rearing temperature. The fat body plays important roles in energy reserve and metabolism during the honeybee brood development. To date, the fat body metabolic changes in prepupae responding to cold stress have not been completely understood. In this study, the ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS)-based non-target metabolome was analyzed between the cold-treated (CT, 20 degrees C, 36 h) and control (CK, 35 degrees C) fat body in prepupal honeybees. The fat body metabolomic data showed that the levels of 1860 and 254 metabolites were significantly increased and decreased, respectively, in cold-stressed prepupae. These altered metabolites, glutamine, glutamic acid, pyroglutamic acid, and oxidized glutathione, were significantly enriched into glutamine metabolism and glutathione metabolism pathways. Furthermore, the expression levels of glutamine metabolism-related genes, glutaminase (GLS), glutamate dehydrogenase (GDH), and gamma-glutamyl transferase (GGT-1 and GGT-7), were significantly decreased in cold-exposed prepupae compared with the control groups. Meanwhile, the oxidized glutathione (GSSG), but not the reduced glutathione (GSH) content, was increased in the cold-exposed group compared with controls. Collectively, our data revealed the fat body metabolomic changes in larva-to-pupa transition when exposed to cold stress. Our data provided new insights into stenothermic honeybee sensitivity to cold, characterized by perturbation of glutamine metabolism and oxidative stress.
Keyword ：
cold stress cold stress fat body fat body honeybees honeybees metabolomics metabolomics oxidative stress oxidative stress
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| GB/T 7714 | Xu, Xinjian , Cao, Mingjie , Zhu, Chenyu et al. Fat Body Metabolome Revealed Glutamine Metabolism Pathway Involved in Prepupal Apis mellifera Responding to Cold Stress [J]. | INSECTS , 2025 , 16 (1) . |
| MLA | Xu, Xinjian et al. "Fat Body Metabolome Revealed Glutamine Metabolism Pathway Involved in Prepupal Apis mellifera Responding to Cold Stress" . | INSECTS 16 . 1 (2025) . |
| APA | Xu, Xinjian , Cao, Mingjie , Zhu, Chenyu , Mo, Lingqing , Huang, Huajiao , Xie, Jiaying et al. Fat Body Metabolome Revealed Glutamine Metabolism Pathway Involved in Prepupal Apis mellifera Responding to Cold Stress . | INSECTS , 2025 , 16 (1) . |
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Melanin is an important component of the body color of honeybees, and its formation changes with the age of a capped brood of bees. However, up to now, the regulatory mechanism of melanin formation in honeybees remains unclear. To analyze the differential expression profile of microRNAs (miRNAs) in worker bees of Apis mellifera and to reveal the regulatory roles of differentially expressed miRNAs (DEmiRNAs) and mRNAs in the formation process of melanin during the capped brood stage, we used sRNA-seq technology and related software to analyze samples from four key developmental stages during the capped brood stage, when body color develops in Apis mellifera, namely, mature larvae (L0), pre-pupae (PP3), early pupae (P6) and mid-pupae (P9). A total of 1291 miRNAs were identified by bioinformatics. Three comparison groups were analyzed: L0 vs. PP3, PP3 vs. P6, and P6 vs. P9. A total of 171, 94, and 19 DEmiRNAs were identified in these groups, respectively, which regulate 1481, 690, and 182 differentially expressed target mRNAs (target DEmRNAs). The functional analysis of target DEmRNAs indicated that DEmiRNAs might regulate the formation of capped brood melanin in honeybees by activating expression changes in key genes in signaling pathways, such as the Wnt signaling pathway, melanogenesis, and the Toll and Imd signaling pathway, through activating miR-315-x, miR-8, ple, yellow family genes, wnt1, etc. Our research provides a theoretical basis for future analysis of the regulatory role of miRNAs in the formation of melanin in honeybees.
Keyword ：
different developmental stages different developmental stages honeybees honeybees melanin melanin miRNA miRNA
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| GB/T 7714 | Zhu, Xiangjie , Tian, Yuanmingyue , Cao, Mingjie et al. A Comparative Transcriptomic Analysis of miRNAs and Their Target Genes During the Formation of Melanin in Apis mellifera [J]. | AGRICULTURE-BASEL , 2025 , 15 (9) . |
| MLA | Zhu, Xiangjie et al. "A Comparative Transcriptomic Analysis of miRNAs and Their Target Genes During the Formation of Melanin in Apis mellifera" . | AGRICULTURE-BASEL 15 . 9 (2025) . |
| APA | Zhu, Xiangjie , Tian, Yuanmingyue , Cao, Mingjie , Zhu, Chenyu , Shang, Jiaqi , Sun, Jiaqi et al. A Comparative Transcriptomic Analysis of miRNAs and Their Target Genes During the Formation of Melanin in Apis mellifera . | AGRICULTURE-BASEL , 2025 , 15 (9) . |
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本发明涉及一种养蜂技术领域，尤其涉及一种蜂箱外免割蜡盖收集雄蜂幼虫或蛹的方法。包括如下步骤：(1)雄蜂幼虫的培育；(2)将得到的幼虫脾或封盖子脾转入恒温恒湿箱中培育，控制温度37±0.5℃，相对湿度75％±5％，并在紧贴雄蜂脾的下方放置托盘来接纳钻出巢房的雄蜂幼虫；(3)直接采收托盘上钻出巢房的雄蜂幼虫，或者将幼虫连同托盘放入恒温恒湿箱中培育后采收蜂蛹；采收的雄蜂大幼虫或蛹立即冷冻保存。该方法利用雄蜂大龄幼虫离开蜂群后在高温胁迫下钻出巢房来生产雄蜂幼虫和蛹，可在12天左右完成生产采收，实现了雄蜂巢脾循环利用、缩短了生产周期、减少了人工成本，提高了雄蜂蛹品质和生产效率。
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| GB/T 7714 | 徐新建 , 周姝婧 , 朱翔杰 et al. 一种蜂箱外免割蜡盖收集雄蜂幼虫或蛹的方法 : CN202311700940.7[P]. | 2023-12-12 . |
| MLA | 徐新建 et al. "一种蜂箱外免割蜡盖收集雄蜂幼虫或蛹的方法" : CN202311700940.7. | 2023-12-12 . |
| APA | 徐新建 , 周姝婧 , 朱翔杰 , 周冰峰 , 伍陈馨子 , 黄锦荣 et al. 一种蜂箱外免割蜡盖收集雄蜂幼虫或蛹的方法 : CN202311700940.7. | 2023-12-12 . |
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<正>我国北方，冬季气候严寒且漫长，蜂群需要在巢内度过冬季。北方蜂群在进入越冬阶段之前，蜂群要在秋季做好充分的准备，才能确保越冬顺利，来年春季蜂群才能更好的恢复和发展群势，为流蜜期生产打下良好的基础
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| GB/T 7714 | 朱翔杰 , 徐新建 , 周姝婧 et al. 北方中华蜜蜂蜂群安全越冬技术 [J]. | 中国蜂业 , 2024 , 75 (11) : 15-17 . |
| MLA | 朱翔杰 et al. "北方中华蜜蜂蜂群安全越冬技术" . | 中国蜂业 75 . 11 (2024) : 15-17 . |
| APA | 朱翔杰 , 徐新建 , 周姝婧 , 付中民 , 周冰峰 , 伍陈馨子 et al. 北方中华蜜蜂蜂群安全越冬技术 . | 中国蜂业 , 2024 , 75 (11) , 15-17 . |
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<正>一、分蜂热的概念及促使分蜂热的主要因素分蜂热是中华蜜蜂饲养管理中较难解决的技术问题，制约了中华蜜蜂饲养管理的规模和效率。养蜂生产必须以强群为基础，分蜂性强的蜂群很难维持强群。分蜂热是指蜂群准备分蜂的状态，突出特点是“怠工”，也就是蜂王产卵减少，泌蜡造脾速度减缓，巢外采集活动积极性减弱。产生分蜂热的蜂群既影响蜂群的增长，又影响养蜂生产，特别是在主要蜜源花期，如果发生分蜂热就会大大影响蜂蜜生产。分蜂热处理不当就会发生分蜂。
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| GB/T 7714 | 朱翔杰 , 徐新建 , 周姝婧 et al. 中华蜜蜂控制分蜂热技术 [J]. | 中国蜂业 , 2024 , 75 (06) : 16-17 . |
| MLA | 朱翔杰 et al. "中华蜜蜂控制分蜂热技术" . | 中国蜂业 75 . 06 (2024) : 16-17 . |
| APA | 朱翔杰 , 徐新建 , 周姝婧 , 付中民 , 姚清花 , 周冰峰 . 中华蜜蜂控制分蜂热技术 . | 中国蜂业 , 2024 , 75 (06) , 16-17 . |
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【目的】明确江西省生境条件下东方蜜蜂遗传多样性水平及遗传分化规律，为促进江西省东方蜜蜂遗传资源的保护与利用，以及我国东方蜜蜂遗传资源的保护和合理布局提供理论依据。【方法】选用37个微卫星标记对江西省10个代表性样点共704群东方蜜蜂进行遗传特征、遗传多样性和遗传分化分析，利用Excel Microsatellite Toolkit 3.1计算观察杂合度（Ho）、期望杂合度（He）、等位基因数（Na）和多态信息含量（PIC），采用PopGene 1.31计算有效等位基因数（Ne）和香农指数（I），通过GenAlEx 6.5和R 4.0.3分别进行主坐标分析（PCoA）和主成分判别分析（DAPC），运用Genepop on Web分析样点间的遗传分化指数（F_(st)），通过F_(st)=1/（1+4Nm）计算基因流（Nm），并以POPTREE2构建UPGMA聚类分析树。【结果】以37个微卫星标记从江西省10个样点704群东方蜜蜂中共检测出374个等位基因，其中PIC高于平均值的有21个微卫星标记（AP243、BI278、AP249、AT185、BI225、AC139、Ap313、AT004、AC045、K0715、AC011、AP189、BI314、Ap085、AT101、244T、Ac-1、Ac-2、Ac-5、Ac-26和Ac-35）。江西省10个样点东方蜜蜂群体的Na为5.2857～6.3125，平均值为5.8247;Ho为0.3951～0.4602，平均值为0.4197;He为0.4211～0.4676，平均值为0.4437;Ne为2.7991～3.2596，平均值为2.9205;PIC为0.3909～0.4366，平均值为0.4133;I为0.9090～1.0106，平均值为0.9542;10个东方蜜蜂样点间的近交系数（F_(is)）为-0.036～0.091,F_(st)为0.0059～0.0294,Nm为8.2534～42.1229。PcoA分析、DAPC分析及UPGMA聚类分析均显示，江西东方蜜蜂尚未发生种群遗传分化。【结论】江西东方蜜蜂遗传多样性处于全国中等水平，未发现江西东方蜜蜂发生种群遗传分化，且在东方蜜蜂遗传分化过程中基因交流的作用大于环境选择。
Keyword ：
东方蜜蜂 东方蜜蜂 微卫星 微卫星 江西 江西 遗传分化 遗传分化 遗传多样性 遗传多样性
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| GB/T 7714 | 朱诗谣 , 周姝婧 , 朱翔杰 et al. 江西东方蜜蜂微卫星DNA的遗传多样性分析 [J]. | 南方农业学报 , 2023 , 54 (08) : 2436-2443 . |
| MLA | 朱诗谣 et al. "江西东方蜜蜂微卫星DNA的遗传多样性分析" . | 南方农业学报 54 . 08 (2023) : 2436-2443 . |
| APA | 朱诗谣 , 周姝婧 , 朱翔杰 , 徐新建 , 苏显冰 , 张芷宁 et al. 江西东方蜜蜂微卫星DNA的遗传多样性分析 . | 南方农业学报 , 2023 , 54 (08) , 2436-2443 . |
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Honeybee is a crucial pollinator in nature, and plays an indispensable role in both agricultural production and scientific research. In recent decades, honeybee was challenged with health problems by biotic and abiotic stresses. As a key ecological factor, temperature has been proved to have an impact on the survival and production efficiency of honeybees. Previous studies have demonstrated that low temperature stress can affect honeybee pupation and shorten adult longevity. However, the molecular mechanism underlying the effects of low temperatures on honeybee growth and development during their developmental period remain poorly understood. In this paper, the weighted gene co-expression analysis (WGCNA) was employed to explore the molecular mechanisms underpinnings of honeybees' respond to low temperatures (20 degrees C) during four distinct developmental stages: large-larvae, prepupae, early-pupae and mid-pupae. Through an extensive transcriptome analysis, thirteen gene co-expression modules were identified and analyzed in relation to honeybee development and stress responses. The darkorange module was found to be associated with low temperature stress, with its genes primarily involved in autophagy-animal, endocytosis and MAPK signaling pathways. Four hub genes were identified within this module, namely, loc726497, loc409791, loc410923, and loc550857, which may contribute to honeybee resistance to low temperature and provide insight into the underlying mechanism. The gene expression patterns of grey60 and black modules were found to correspond to the developmental stages of prepupae and early-pupae, respectively, with the hub genes loc409494, loc725756, loc552457, loc726158, Ip3k and Lcch3 in grey60 module likely involved in brain development, and the hub genes loc410555 in black module potentially related to exoskeleton development. The brown module genes exhibited a distinct pattern of overexpression in mid-pupae specimens, with genes primarily enriched in oxidative phosphorylation, citrate cycle and other pathways, which may be related to the formation of bee flying muscle. No related gene expression module was found for mature larvae stage. These findings provide valuable insights into the developmental process of honeybees at molecular level during the capped brood stage.
Keyword ：
cold response cold response development development honeybee honeybee hub genes hub genes pupae pupae WGCNA WGCNA
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| GB/T 7714 | Zhu, Chenyu , Xu, Xinjian , Zhou, Shujing et al. WGCNA based identification of hub genes associated with cold response and development in Apis mellifera metamorphic pupae [J]. | FRONTIERS IN PHYSIOLOGY , 2023 , 14 . |
| MLA | Zhu, Chenyu et al. "WGCNA based identification of hub genes associated with cold response and development in Apis mellifera metamorphic pupae" . | FRONTIERS IN PHYSIOLOGY 14 (2023) . |
| APA | Zhu, Chenyu , Xu, Xinjian , Zhou, Shujing , Zhou, Bingfeng , Liu, Yiming , Xu, Hongzhi et al. WGCNA based identification of hub genes associated with cold response and development in Apis mellifera metamorphic pupae . | FRONTIERS IN PHYSIOLOGY , 2023 , 14 . |
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The honeybees are the most important pollinator in the production of crops and fresh produce. Temperature affects the survival of honeybees, and determines the quality of their development, which is of great significance for beekeeping production. Yet, little was known about how does low temperature stress during development stage cause bee death and any sub-lethal effect on subsequent. Early pupal stage is the most sensitive stage to low temperature in pupal stage. In this study, early pupal broods were exposed to 20 degrees C for 12, 16, 24, and 48 h, followed by incubation at 35 degrees C until emergence. We found that 48 h of low temperature duration cause 70% of individual bees to die. Although the mortality at 12 and 16 h seems not very high, the association learning ability of the surviving individuals was greatly affected. The brain slices of honeybees showed that low temperature treatment could cause the brain development of honeybees to almost stop. Gene expression profiles between low temperature treatment groups (T24, T48) and the control revealed that 1,267 and 1,174 genes were differentially expressed respectively. Functional enrichment analysis of differentially expressed genes showed that the differential expression of Map3k9, Dhrs4, and Sod-2 genes on MAPK and peroxisome signaling pathway caused oxidative damage to the honeybee head. On the FoxO signal pathway, InsR and FoxO were upregulated, and JNK, Akt, and Bsk were downregulated; and on the insect hormone synthesis signal pathway, Phm and Spo genes were downregulated. Therefore, we speculate that low temperature stress affects hormone regulation. It was detected that the pathways related to the nervous system were Cholinergic synapse, Dopaminergic synapse, GABAergic synapse, Glutamatergic synapse, Serotonergic synapse, Neurotrophin signaling pathway, and Synaptic vesicle cycle. This implies that the synaptic development of honeybees is quite possibly greatly affected by low temperature stress. Understanding how low temperature stress affects the physiology of bee brain development and how it affects bee behavior provide a theoretical foundation for a deeper comprehension of the temperature adaptation mechanism that underlies the "stenothermic" development of social insects, and help to improve honeybee management strategies to ensure the healthy of colony.
Keyword ：
Apis mellifera Apis mellifera association learning association learning cold-temperature stress cold-temperature stress development development pupae stage pupae stage
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| GB/T 7714 | Zhu, Chenyu , Li, Han , Xu, Xinjian et al. The mushroom body development and learning ability of adult honeybees are influenced by cold exposure during their early pupal stage [J]. | FRONTIERS IN PHYSIOLOGY , 2023 , 14 . |
| MLA | Zhu, Chenyu et al. "The mushroom body development and learning ability of adult honeybees are influenced by cold exposure during their early pupal stage" . | FRONTIERS IN PHYSIOLOGY 14 (2023) . |
| APA | Zhu, Chenyu , Li, Han , Xu, Xinjian , Zhou, Shujing , Zhou, Bingfeng , Li, Xiang et al. The mushroom body development and learning ability of adult honeybees are influenced by cold exposure during their early pupal stage . | FRONTIERS IN PHYSIOLOGY , 2023 , 14 . |
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