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学者姓名:缪晓青
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Abstract :
Salmonella enterica serovar Typhimurium (S. Typhimurium) is a common food-borne pathogen that commonly causes gastroenteritis in humans and animals. Apis laboriosa honey (ALH) harvested in China has significant antibacterial activity against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. We hypothesize that ALH has antibacterial activity against S. Typhimurium. The physicochemical parameters, minimum inhibitory and bactericidal concentrations (MIC and MBC) and the possible mechanism were determined. The results showed that there were significantly different physicochemical parameters, including 73 phenolic compounds, among ALH samples harvested at different times and from different regions. Their antioxidant activity was affected by their components, especially total phenol and flavonoid contents (TPC, TFC), which had a high correlation with antioxidant activities except for the O-2- assay. The MIC and MBC of ALH against S. Typhimurium were 20-30% and 25-40%, respectively, which were close to those of UMF5+ manuka honey. The proteomic experiment revealed the possible antibacterial mechanism of ALH1 at IC50 (2.97%, w/v), whose antioxidant activity reduced the bacterial reduction reaction and energy supply, mainly by inhibiting the citrate cycle (TCA cycle), amino acid metabolism pathways and enhancing the glycolysis pathway. The results provide a theoretical basis for the development of bacteriostatic agents and application of ALH.
Keyword :
antibacterial activity antibacterial activity antioxidant activity antioxidant activity Apis laboriosa honey Apis laboriosa honey proteomics proteomics Salmonella Typhimurium Salmonella Typhimurium
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| GB/T 7714 | Tan, Weihua , Tian, Yuanyuan , Zhang, Qingya et al. Antioxidant and antibacterial activity of Apis laboriosa honey against Salmonella enterica serovar Typhimurium [J]. | FRONTIERS IN NUTRITION , 2023 , 10 . |
| MLA | Tan, Weihua et al. "Antioxidant and antibacterial activity of Apis laboriosa honey against Salmonella enterica serovar Typhimurium" . | FRONTIERS IN NUTRITION 10 (2023) . |
| APA | Tan, Weihua , Tian, Yuanyuan , Zhang, Qingya , Miao, Siwei , Wu, Wenrong , Miao, Xiaoqing et al. Antioxidant and antibacterial activity of Apis laboriosa honey against Salmonella enterica serovar Typhimurium . | FRONTIERS IN NUTRITION , 2023 , 10 . |
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Propolis is resinous natural product produced by Western honeybees using beeswax and plant and bud exudates, which has a wide range of biological activities, including antioxidation, antibacterial, anti-inflammation, immune regulation, antitumor, and so on. Diffuse large B-cell lymphoma (DLBCL) is an aggressive cancer, and accounts for about 30% of all lymphomas. The effect of poplar propolis on DLBCL has not been reported. The IC50 of propolis on the proliferation of DLBCL SU-DHL-2 cell line and its proteins and gene expressions were detected by CCK-8 kit, label-free proteomic, and RT-PCR. The results showed that the IC50 of propolis at the 5 x l05/mL cell for 24 h was 5.729 mu g/mL. Label-free-based proteomics analysis showed that there were 115 differentially expressed proteins (61 up-regulated and 54 down-regulated proteins) between IC50 dose-treated and solvent control groups. There were 32.47% differential proteins located in the nucleus, 20.78% in the cytoplasm, and 14.29% in mitochondria. The most significant different pathway (p = 0.0016) of protein enrichment was ferroptosis (including glutamate-cysteine ligase regulatory subunit, ferritin, and heme oxygenase). The relative expression trend of 17 of the total 22 genes selected according to proteomics results was in line with their encoded protein. The highest protein-protein interaction was serine/threonine-protein kinase PLK, which interacted with 16 differential proteins. In conclusion, poplar propolis inhibited SU-DHL-2 cells via ferroptosis pathway, accelerating cell death and down-regulated serine/threonine-protein kinase PLK1, affecting apoptosis of cell. This result provides a theoretical basis for the treatment of DLBCL using propolis.
Keyword :
diffuse large B-cell lymphoma diffuse large B-cell lymphoma ferroptosis ferroptosis propolis propolis proteomics proteomics SU-DHL-2 cell line SU-DHL-2 cell line
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| GB/T 7714 | Liu, Xiaoqing , Tian, Yuanyuan , Yang, Ao et al. Antitumor Effects of Poplar Propolis on DLBCL SU-DHL-2 Cells [J]. | FOODS , 2023 , 12 (2) . |
| MLA | Liu, Xiaoqing et al. "Antitumor Effects of Poplar Propolis on DLBCL SU-DHL-2 Cells" . | FOODS 12 . 2 (2023) . |
| APA | Liu, Xiaoqing , Tian, Yuanyuan , Yang, Ao , Zhang, Chuang , Miao, Xiaoqing , Yang, Wenchao . Antitumor Effects of Poplar Propolis on DLBCL SU-DHL-2 Cells . | FOODS , 2023 , 12 (2) . |
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研究蜂针疗法治疗腱鞘囊肿的疗效。对腱鞘囊肿患者囊肿部位采用直接活蜂蜇刺进行治疗,经过3~10次的治疗,14例患者中,痊愈12例,占85.71%;好转2例,占14.29%;总有效率100%。随访1年无复发。蜂针疗法治疗腱鞘囊肿疗效满意。
Keyword :
腱鞘囊肿 腱鞘囊肿 蜂针疗法 蜂针疗法
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| GB/T 7714 | 程林兵 , 缪晓青 , Chen Ao . 蜂针疗法治疗腱鞘囊肿 [C] //2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . 2021 . |
| MLA | 程林兵 et al. "蜂针疗法治疗腱鞘囊肿" 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
| APA | 程林兵 , 缪晓青 , Chen Ao . 蜂针疗法治疗腱鞘囊肿 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
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[目的] Takeout(TO)是一种昆虫节律性调控输出基因,广泛分布于涉及到化学感受和营养功能的相关组织。TO基因主要参与昆虫的生长发育、行为调节及多种生理代谢过程。为探究TO基因是否参与社会性昆虫的劳动分工,我们克隆并分析了中华蜜蜂Apis cerana TO基因的编码框序列,测定了该基因在哺育蜂和采集蜂各个组织部位的mRNA表达水平,旨在为深入研究该基因的功能提供参考。[方法]本研究通过RT-PCR的方法首次克隆获得AcTO1基因的cDNA序列,利用生物信息学软件分析了该基因的核苷酸序列和氨基酸序列,并利用荧光定量PCR (qPCR)技术检测了该基因在中华蜜蜂3种采集蜂(18日龄正常采集蜂、22日龄正常采集蜂和7日龄提前采集蜂)和2种哺育蜂(7日龄正常哺育蜂和22日龄超龄哺育蜂)头部、胸部和腹部的表达量。[结果]本研究克隆获得的中华蜜蜂TO(命名为AcTO1)基因的cDNA序列长度为1058 bp,开放阅读框(ORF)长度为738 bp,编码246个氨基酸,预测蛋白分子量为27.09 kDa,理论等电点为8.40。AcTO1蛋白的信号肽位于1-17位氨基酸,无跨膜结构,属于分泌型蛋白。AcTO1的氨基酸序列种含有一个JHBP超家族保守结构域。qPCR结果显示,AcTO1在3种采集蜂和2种哺育蜂的各组织均有表达,其中表达量由高到低依次为头部、胸部、腹部。AcTO1基因的表达具有组织依赖型,主要表达于头部,且所有采集蜂的头部表达量均高于哺育蜂,由此说明该基因可能参与蜜蜂的劳动分工。[结论]本研究克隆了中华蜜蜂takeout基因的全长cDNA序列,并分析了该基因的序列特征和表达谱,结果表明该基因可能参与调控蜜蜂的劳动分工。
Keyword :
takeout基因 takeout基因 劳动分工 劳动分工 基因克隆 基因克隆 蜜蜂 蜜蜂
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| GB/T 7714 | 李英娇 , 陈艺杰 , 周贺 et al. 中华蜜蜂Takeout(AcTO1)基因的克隆和表达分析 [C] //2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . 2021 . |
| MLA | 李英娇 et al. "中华蜜蜂Takeout(AcTO1)基因的克隆和表达分析" 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
| APA | 李英娇 , 陈艺杰 , 周贺 , 李江红 , 缪晓青 . 中华蜜蜂Takeout(AcTO1)基因的克隆和表达分析 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
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[目的]克隆中华蜜蜂(Apis cerana cerana) AcerOBP7基因并分析其相关生物学信息。[方法]收集中华蜜蜂样本,提取总RNA,反转录制备cDNA。设计特异性引物,PCR扩增AcerOBP7基因的ORF序列。利用多种生物信息学软件对编码蛋白的理化性质和结构特征进行了预测和分析。[结果]获得中华蜜蜂气味结合蛋白基因AcerOBP7的cDNA序列。AcerOBP7的开放阅读框(ORF)长438 bp,编码145个氨基酸。存在信号肽,无跨膜结构,在53-140个氨基酸之间有一个昆虫气味结合蛋白家族的PBP-GOBP家族的保守结构域,信号肽源于氨基酸中的1-20位,其对应的剪切位点落在氨基酸中的20位与21位间。有一些相对清晰的疏水区。[结论] AcerOBP7属于Classical OBP亚家族,具有典型的PBP-GOBP家族结构,是一种分泌蛋白。
Keyword :
AcerOBP7 AcerOBP7 中华蜜蜂 中华蜜蜂 分泌性蛋白 分泌性蛋白 基因克隆 基因克隆 生物信息学 生物信息学
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| GB/T 7714 | 李英娇 , 陈艺杰 , 周贺 et al. 中华蜜蜂气味结合蛋白基因AcerOBP7的克隆及序列分析 [C] //2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . 2021 . |
| MLA | 李英娇 et al. "中华蜜蜂气味结合蛋白基因AcerOBP7的克隆及序列分析" 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
| APA | 李英娇 , 陈艺杰 , 周贺 , 李江红 , 缪晓青 . 中华蜜蜂气味结合蛋白基因AcerOBP7的克隆及序列分析 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
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蜜蜂采集行为对农作物的授粉和蜂群的生存和发展都非常重要。为探究西方蜜蜂(Apis mellifera)工蜂在夏季的出巢活动规律,本研究采用手机于设定的时间内在蜂巢门口固定拍摄视频的方式对返巢蜜蜂进行拍摄,进而记录下蜜蜂的日出巢活动情况。结果表明,蜜蜂的采集活动主要集中在上午,清晨温度较低,采集蜂数量很少,随着温度逐渐升高,采集蜂数量逐渐增加,至上午10点左右采集蜂数量最高,达采集高峰,随后逐渐减少,到12点~14点采集数量略增加,随后急剧下降,到下午6点左右基本就没有蜜蜂出巢活动。本研究系统的研究了夏季西方蜜蜂(Apis mellifera)工蜂的日出巢采集活动规律,对了解西方蜜蜂(Apis mellifera)的出巢采集行为、相关的生物学特性以及授粉实践等都具有积极的作用。
Keyword :
出巢 出巢 夏季 夏季 活动 活动 西方蜜蜂 西方蜜蜂
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| GB/T 7714 | 李英娇 , 陈艺杰 , 周贺 et al. 福州地区西方蜜蜂(Apis mellifera)工蜂夏季采集活动规律研究 [C] //2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . 2021 . |
| MLA | 李英娇 et al. "福州地区西方蜜蜂(Apis mellifera)工蜂夏季采集活动规律研究" 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
| APA | 李英娇 , 陈艺杰 , 周贺 , 缪晓青 , 李江红 . 福州地区西方蜜蜂(Apis mellifera)工蜂夏季采集活动规律研究 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
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级型分化是指在社会性昆虫中,个体性别相同,但在形态结构、职能、生理和行为等方面有明显差异的现象。级型分化是昆虫多型现象的一种典型的表现形式。在环境因素的诱导下,昆虫表现出形形色色的多型性现象。在蜂群中,蜂王和工蜂都是由受精卵发育而成的雌性蜂,同样是二倍体受精卵,同样的基因型,却会产生具不同级型特征的蜂王和工蜂个体。本文从营养、激素、基因表达、蛋白质和表观遗传等方面对决定蜜蜂级型分化的分子机制进行了综述,并对以后的研究方向进行了展望。
Keyword :
基因表达 基因表达 激素 激素 级型分化 级型分化 营养 营养 蛋白质 蛋白质 蜜蜂 蜜蜂 表观遗传 表观遗传
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| GB/T 7714 | 李英娇 , 陈艺杰 , 周贺 et al. 蜜蜂级型分化研究进展 [C] //2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . 2021 . |
| MLA | 李英娇 et al. "蜜蜂级型分化研究进展" 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
| APA | 李英娇 , 陈艺杰 , 周贺 , 缪晓青 , 李江红 . 蜜蜂级型分化研究进展 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(下册) . (2021) . |
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为评估黑小蜜蜂蜂蜜的理化性质和抑菌活性,本文用AOAC等方法测定黑小蜜蜂蜂蜜的理化指标、DNA高通量条形码法鉴定其植物来源,琼脂井扩散法评估其对金黄色葡萄球菌(ATCC6538)、大肠杆菌(ATCC8739)和紫色色杆菌(ATCC12472)的抑菌活性。结果表明:同一时间地点收集的三个黑小蜜蜂蜂蜜的水分、灰分、pH、酸度、游离酸、内酯酸、葡萄糖、果糖、淀粉酶活性、5-羟甲基糠醛含量,电导率有显著差异,麦芽糖和蔗糖含量没有显著差异。各样品的植物来源种类和数量差异显著,三个蜂蜜样品没有共有植物来源,AAH1和AAH2仅有一种共有植物来源蓝花野茼蒿(Crassocephalumrubens),基因丰度分别为16.16%和4.15%,AAH1和AAH3仅有一种共有植物来源白饭树(Flueggeavirosa),基因丰度分别为2.06%和31.32%。三个黑小蜜蜂蜂蜜中AAH3对金黄色葡萄球菌的抑菌活性最强且优于麦卢卡蜂蜜,AAH3对大肠杆菌的抑菌活性与MH1蜂蜜无显著差异且优于其他蜂蜜,AAH1对紫色杆菌的抑菌活性最强但是弱于MH3、MH1蜂蜜。结论:黑小蜜蜂蜂蜜具有良好抑菌效果。研究结果可以为黑小蜜蜂蜂蜜的开发和利用提供理论依据。
Keyword :
DNA条形码 DNA条形码 抑菌活性 抑菌活性 植物来源 植物来源 理化指标 理化指标 黑小蜜蜂蜂蜜 黑小蜜蜂蜂蜜
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| GB/T 7714 | 沈敏 , 匡海鸥 , 刘晓青 et al. 黑小蜜蜂蜂蜜的理化性质、植物来源和抑菌活性研究 [C] //2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(上册) . 2021 . |
| MLA | 沈敏 et al. "黑小蜜蜂蜂蜜的理化性质、植物来源和抑菌活性研究" 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(上册) . (2021) . |
| APA | 沈敏 , 匡海鸥 , 刘晓青 , 张创 , 缪晓青 , 杨文超 . 黑小蜜蜂蜂蜜的理化性质、植物来源和抑菌活性研究 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(上册) . (2021) . |
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研究五味子蜂花粉提取物(Schisandra chinensis Bee Pollen Extract,SCBPE)对异丙肾上腺素(ISO)诱导H9c2心肌细胞肥大的干预作用。高、中、低浓度SCBPE预处理细胞后进行ISO损伤,通过细胞骨架染色、细胞面积计算、总蛋白含量测定以及心肌细胞肥大标志物的基因表达水平检测,探究SCBPE对ISO诱导H9c2心肌细胞肥大的干预作用。结果表明,与模型组相比,SCBPE干预组细胞表面积显著缩小,高浓度SCBPE组细胞的总蛋白含量与ANP、BNP及β-MHC的表达水平显著降低。上述结果表明SCBPE具有减轻ISO诱导H9c2心肌细胞肥大的作用。
Keyword :
五味子蜂花粉 五味子蜂花粉 异丙肾上腺素 异丙肾上腺素 心肌细胞肥大 心肌细胞肥大
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| GB/T 7714 | 陈立府 , 李志良 , 孟菲 et al. 五味子蜂花粉干预ISO诱导心肌细胞肥大的作用研究 [C] //2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(上册) . 2021 . |
| MLA | 陈立府 et al. "五味子蜂花粉干预ISO诱导心肌细胞肥大的作用研究" 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(上册) . (2021) . |
| APA | 陈立府 , 李志良 , 孟菲 , 谢仕慧 , 史培颖 , 缪晓青 . 五味子蜂花粉干预ISO诱导心肌细胞肥大的作用研究 2021年中国(广西梧州)蜂业博览会暨全国蜂产品市场信息交流会论文集(上册) . (2021) . |
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We hypothesize that the wild honey (Apis dorsata honey (ADH), A. florea honey, A. laboriosa honey and A. andreniformis honey) had high antibacterial activity to defend against degradation. The entomological proteins, botanical sources and antibacterial activities of wild honey were determined via a label-free proteome, DNA metabarcoding and agar well diffusion assay. The results showed that there were significant differences among physico-chemical properties, proteins and plant sources of honey samples from different honey bee species. All the honey samples diluted to a 90% moisture content inhibited Staphylococcus aureus, Escherichia coli and Bacillus subtilis with the exception of 90% ADH. Honey solutions inhibited Chromobacterium violaceum, with exception of ADH and some other species honey with different moisture contents. The differences in antibacterial activity were attributed to differences in the botanical sources, entomological proteins of the honey. The speciesspecific proteins in honey from each species can be used for their identification
Keyword :
Antibacterial activity Antibacterial activity Botanical source Botanical source Entomological proteome Entomological proteome Honey Honey Manuka honey Manuka honey
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| GB/T 7714 | Yang, Wenchao , Shen, Min , Kuang, Haiou et al. The botanical sources, entomological proteome and antibiotic properties of wild honey [J]. | INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES , 2021 , 67 . |
| MLA | Yang, Wenchao et al. "The botanical sources, entomological proteome and antibiotic properties of wild honey" . | INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 67 (2021) . |
| APA | Yang, Wenchao , Shen, Min , Kuang, Haiou , Liu, Xiaoqing , Zhang, Chuang , Tian, Yuanyuan et al. The botanical sources, entomological proteome and antibiotic properties of wild honey . | INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES , 2021 , 67 . |
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