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学者姓名：陈伟
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Abstract ：
Rubisco activase (RCA) is an ATP-dependent enzyme that plays a crucial role in plant stress responses by regulating the catalytic activity of Rubisco. However, the alternative splicing and functional characteristics of the RCA gene exhibit notable species-specific diversity. The variable splice forms and functions of the RCA gene in mangrove plants remain poorly understood. Herein, we cloned the RCA cDNA in the leaves of mangrove plant Kandelia candel (L.) in response to combined flooding and salinity stress, and performed systematic expression analysis and functional validation. Our results demonstrated that the RCA gene undergoes alternative splicing to produce two isoforms, designated as KcRCAl (GenBank accession: MG492021) and KcRCAs (GenBank accession: MG492022), respectively. The KcRCAl encodes a 440-amino acid protein (42.49 kDa) belonging to the beta-isoforms, while KcRCAs encodes a 474-amino acid protein (46.10 kDa) classified as the alpha-isoforms. Moreover, protein structure analysis revealed that both isoforms contain phosphorylation and lysine acetylation modification sites. Phylogenetic analysis indicated that KcRCA shares the closest evolutionary relationship with RCA from Cicer arietinum (chickpea) and Durio zibethinus (durian). Furthermore, RT-qPCR analysis revealed that the expression levels of KcRCAl and KcRCAs were significantly upregulated in K. Candel leaves under the combined stress condition. The following functional validation studies in transgenic Arabidopsis demonstrated that overexpression of the KcRCA cDNA enhances the plant's tolerance to resist flooding and salinity stress while improving antioxidant capacity and increasing RCA and Rubisco activity, thereby maintaining photosynthetic efficiency under combined flooding and salinity stress. Our study not only provides new experimental evidence for understanding the molecular mechanisms of plant flooding and salinity stress, but also offers theoretical foundations for breeding flooding- and salinity-tolerant crops.
Keyword ：
combined stress combined stress flooding and salinity flooding and salinity functional analysis functional analysis gene cloning gene cloning Kandelia candel Kandelia candel rubisco activase rubisco activase
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| GB/T 7714 | Xing, Jianhong , Pan, Dezhuo , Li, Changfu et al. Functional Characterization of Rubisco Activase Genes in Kandelia candel Under the Stress of Flooding and Salinity [J]. | AGRICULTURE-BASEL , 2025 , 15 (21) . |
| MLA | Xing, Jianhong et al. "Functional Characterization of Rubisco Activase Genes in Kandelia candel Under the Stress of Flooding and Salinity" . | AGRICULTURE-BASEL 15 . 21 (2025) . |
| APA | Xing, Jianhong , Pan, Dezhuo , Li, Changfu , Yan, Shufeng , Chen, Wei , Zhang, Juncheng et al. Functional Characterization of Rubisco Activase Genes in Kandelia candel Under the Stress of Flooding and Salinity . | AGRICULTURE-BASEL , 2025 , 15 (21) . |
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Cannabis glandular trichomes (GTs) are economically and biotechnologically important structures that have a remarkable morphology and capacity to produce, store, and secrete diverse classes of secondary metabolites. However, our understanding of the developmental changes and the underlying molecular processes involved in cannabis GT development is limited. In this study, we developed Cannabis Glandular Trichome Detection Model (CGTDM), a deep learning-based model capable of differentiating and quantifying three types of cannabis GTs with a high degree of efficiency and accuracy. By profiling at eight different time points, we captured dynamic changes in gene expression, phenotypes, and metabolic processes associated with GT development. By integrating weighted gene co-expression network analysis with CGTDM measurements, we established correlations between phenotypic variations in GT traits and the global transcriptome profiles across the developmental gradient. Notably, we identified a module containing methyl jasmonate (MeJA)-responsive genes that significantly correlated with stalked GT density and cannabinoid content during development, suggesting the existence of a MeJA-mediated GT formation pathway. Our findings were further supported by the successful promotion of GT development in cannabis through exogenous MeJA treatment. Importantly, we have identified CsMYC4 as a key transcription factor that positively regulates GT formation via MeJA signaling in cannabis. These findings provide novel tools for GT detection and counting, as well as valuable information for understanding the molecular regulatory mechanism of GT formation, which has the potential to facilitate the molecular breeding, targeted engineering, informed harvest timing, and manipulation of cannabinoid production.
Keyword ：
cannabis cannabis deep learning deep learning glandular trichomes glandular trichomes MeJA signaling MeJA signaling transcription factor transcription factor transcriptomics transcriptomics
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| GB/T 7714 | Huang, Xiaoqin , Chen, Wei , Zhao, Yuqing et al. Deep learning-based quantification and transcriptomic profiling reveal a methyl jasmonate-mediated glandular trichome formation pathway in Cannabis sativa [J]. | PLANT JOURNAL , 2024 , 118 (4) : 1155-1173 . |
| MLA | Huang, Xiaoqin et al. "Deep learning-based quantification and transcriptomic profiling reveal a methyl jasmonate-mediated glandular trichome formation pathway in Cannabis sativa" . | PLANT JOURNAL 118 . 4 (2024) : 1155-1173 . |
| APA | Huang, Xiaoqin , Chen, Wei , Zhao, Yuqing , Chen, Jingjing , Ouyang, Yuzeng , Li, Minxuan et al. Deep learning-based quantification and transcriptomic profiling reveal a methyl jasmonate-mediated glandular trichome formation pathway in Cannabis sativa . | PLANT JOURNAL , 2024 , 118 (4) , 1155-1173 . |
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Glucose oxidase (GOX) is a representative compound found in most insect saliva that can suppress plant-defensive responses. However, little is known about the origin and role of GOX in the crucifer-specialized pest Plutella xylostella. In this study, we showed obvious regurgitation from the larval gut of P. xylostella and identified abundant peptides highly similar to known GOX. Three PxGOX genes were verified with PxGOX2 preferentially expressed in the gut. The heterologously expressed PxGOX2 confirmed its function to be a GOX, and it was detected in plant wounds together with the gut regurgitant. Further experiments revealed that PxGOX2 functioned as an effector and may suppress defensive responses in plant through the production of H2O2, which modulates levels of antagonistic salicylic acid and jasmonic acid. However, excessive H2O2 in the host plant may be neutralized by peroxidase, thus forming defensive feedback. Our findings provided new insights into understanding the GOX-mediated insect-plant interactions.
Keyword ：
gene editing gene editing gut regurgitant gut regurgitant oral secretions oral secretions plant defense plant defense ROS-scavenging ROS-scavenging
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| GB/T 7714 | Yang, Feiying , Jing, Xiaodong , Dong, Renfu et al. Glucose Oxidase of a Crucifer-Specialized Insect: A Potential Role in Suppressing Plant Defense via Modulating Antagonistic Plant Hormones [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2023 , 71 (45) : 17469-17483 . |
| MLA | Yang, Feiying et al. "Glucose Oxidase of a Crucifer-Specialized Insect: A Potential Role in Suppressing Plant Defense via Modulating Antagonistic Plant Hormones" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 71 . 45 (2023) : 17469-17483 . |
| APA | Yang, Feiying , Jing, Xiaodong , Dong, Renfu , Zhou, Li , Xu, Xuejiao , Dong, Yuhong et al. Glucose Oxidase of a Crucifer-Specialized Insect: A Potential Role in Suppressing Plant Defense via Modulating Antagonistic Plant Hormones . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2023 , 71 (45) , 17469-17483 . |
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以普通油茶(Camellia oleifera)优良无性系‘闽43’为实验材料,进行生理试验和酶活性检测,采用定量蛋白质组学技术对油茶成熟过程中3个时期的籽仁进行分析,探讨油茶籽油脂代谢的调控机制。研究发现,共有284个蛋白差异表达,依据功能分为17类,其中脂质代谢与运输相关蛋白多数呈现下调表达。硬脂酰-ACP去饱和酶(SAD)和胆碱激酶(CK)蛋白表达量的下调,与不饱和脂肪酸生成速率的减缓有关;在油茶果实成熟过程中的第二个阶段,脂肪酶(LPS)活性的下调造成游离脂肪酸的产生减少。由于长链脂酰辅酶A合成酶(LACS)蛋白表达量的下调,亚油酸和亚麻酸以游离脂肪酸的形式存在较长时间,促使脂氧合酶(LOX)的活性上升,导致该时期亚油酸和亚麻酸含量的下降。研究结果表明,油茶籽成熟过程中,不饱和脂肪酸含量的调控与油脂代谢相关蛋白表达模式和酶活性的变化密切相关。研究结果为选育高品质的油茶品种提供理论依据。
Keyword ：
不饱和脂肪酸 不饱和脂肪酸 差异表达蛋白 差异表达蛋白 油脂代谢 油脂代谢 油茶 油茶 蛋白质组 蛋白质组
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| GB/T 7714 | 沈军 , 邱智敏 , 余玉云 et al. 油茶籽成熟过程中油脂代谢的蛋白质组学分析 [J]. | 植物生理学报 , 2023 , 59 (07) : 1398-1406 . |
| MLA | 沈军 et al. "油茶籽成熟过程中油脂代谢的蛋白质组学分析" . | 植物生理学报 59 . 07 (2023) : 1398-1406 . |
| APA | 沈军 , 邱智敏 , 余玉云 , 李志真 , 陈伟 , 陈世品 . 油茶籽成熟过程中油脂代谢的蛋白质组学分析 . | 植物生理学报 , 2023 , 59 (07) , 1398-1406 . |
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Emerging contaminants are characterized by toxicity, environmental durability, and bioaccumulation. How to effectively remove them has attracted tremendous attention in the field of environmental science and engineering. Nanomaterial-microbe coupling systems driven by photo-electrochemistry have high degradation efficiency and low cost by combining the advantages of nanomaterials and microorganisms in pollutant treatment. This review summarizes the recent development of the nanomaterial-microbe system for the removal of emerging contaminants, which is divided into exogenous nanomaterial-microbe and endogenous nanomaterial-microbe. The interaction mechanism of materials and microorganisms is addressed from the particular perspective of extracellular electron transfer and redox reactions, and the advantages and challenges of the pollutants treatment are analyzed. Furthermore, future applications in the degradation of emerging contaminants in environmental fields have been prospected.
Keyword ：
Emerging contaminant Emerging contaminant Extracellular electron transfer Extracellular electron transfer Microbe Microbe Nanomaterial Nanomaterial
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| GB/T 7714 | Ren, Chongyuan , Bai, Rui , Chen, Wei et al. Advances in Nanomaterial-microbe Coupling System for Removal of Emerging Contaminants [J]. | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES , 2023 , 39 (3) : 389-394 . |
| MLA | Ren, Chongyuan et al. "Advances in Nanomaterial-microbe Coupling System for Removal of Emerging Contaminants" . | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 39 . 3 (2023) : 389-394 . |
| APA | Ren, Chongyuan , Bai, Rui , Chen, Wei , Li, Junpeng , Zhou, Xudong , Tian, Xiaochun et al. Advances in Nanomaterial-microbe Coupling System for Removal of Emerging Contaminants . | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES , 2023 , 39 (3) , 389-394 . |
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| GB/T 7714 | Li, Junpeng , Bai, Rui , Chen, Wei et al. Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char (vol 447, 130772, 2023) [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 455 . |
| MLA | Li, Junpeng et al. "Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char (vol 447, 130772, 2023)" . | JOURNAL OF HAZARDOUS MATERIALS 455 (2023) . |
| APA | Li, Junpeng , Bai, Rui , Chen, Wei , Ren, Chongyuan , Yang, Fan , Tian, Xiaochun et al. Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char (vol 447, 130772, 2023) . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 455 . |
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Mineralization of lead ions (Pb2+) to pyromorphite using phosphorus-containing materials is an effective way to remediate lead (Pb) contamination. Bone char is rich in phosphorus, but its immobilization of Pb2+ is limited by poor phosphate release. To utilize the phosphorus in bone char and provide a suitable growth environment for phosphate-solubilizing bacteria, bone char and Pseudomonas rhodesiae HP-7 were encapsulated into bio-beads, and the immobilization performance and mechanism of Pb in solution and soil by bio-beads were investigated. The results showed that 137 mg/g of phosphorus was released from bone char in the presence of the HP-7 strain. Pb2+ removal efficiency reached 100 % with an initial Pb2+ concentration of 1 mM, bone char content of 6 g/L, and bio-bead dosage of 1 %. Most Pb2+ was immobilized on the surface of the bio-beads as Pb5(PO4)3Cl. The soil remediation experiments showed a 34 % reduction in the acid-soluble fraction of Pb. The bio-beads showed good stability in long-term (30 d) soil remediation. The present study shows that bone char can be turned into an efficient Pb immobilization material in the presence of phosphate-solubilizing bacteria. Thus, bio-beads are expected to be used in the remediation of Pb-contaminated environments.
Keyword ：
Bioremediation Bioremediation Bone char Bone char Encapsulation Encapsulation Lead Lead Phosphate-solubilizing bacteria Phosphate-solubilizing bacteria
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| GB/T 7714 | Li, Junpeng , Bai, Rui , Chen, Wei et al. Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 447 . |
| MLA | Li, Junpeng et al. "Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char" . | JOURNAL OF HAZARDOUS MATERIALS 447 (2023) . |
| APA | Li, Junpeng , Bai, Rui , Chen, Wei , Ren, Chongyuan , Yang, Fan , Tian, Xiaochun et al. Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 447 . |
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A valuable plant, Cyclobalanopsis gilva, (C. gilva) has a low germination rate (below 50%) under its natural habitations. In order to examine the reasons for the low germination rate, the seeds of C. gilva (germinated and non-germinated) were evaluated using comparative proteomics analysis. A total of 3078 differentially abundant proteins (DAPs) were identified through a label-free method; most DAPs up-accumulated in germinated seeds were related to carbohydrates metabolism. Furthermore the proteins related to the signals, stress, and protein metabolism showed up-accumulation in germinated and no abundance or down-accumulation in non-germinated seeds. Enzyme activity of HK, PGK, PFK, and PK from glycolysis in SG-Control samples were 1.7-,11-, 1.4-, and 1.3-times higher compared with those in control ones while CS, NAD-MDH, alpha-KGDH, and ICDH from the TCA cycle in SG-Control samples were 3, 1.1, 1.2, and 1.2 times higher than those in NG-Control ones. The beta-amylase activity was 4-fold higher in successfully germinated seeds compared to non-germinated seeds. Interestingly, alpha-amylase did not show significant changes in protein abundance and enzyme activity among the three samples. The present findings reveal that unsuccessful germination of C. gilva seeds is due to lack of energy. (C) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.
Keyword ：
Carbohydrate metabolism Carbohydrate metabolism Glycolysis Glycolysis Proteomics Proteomics Seed germination Seed germination
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| GB/T 7714 | Zaynab, Madiha , Pan, Dezhuo , Fatima, Mahpara et al. Proteomics analysis of Cyclobalanopsis gilva provides new insights of low seed germination [J]. | BIOCHIMIE , 2021 , 180 : 68-78 . |
| MLA | Zaynab, Madiha et al. "Proteomics analysis of Cyclobalanopsis gilva provides new insights of low seed germination" . | BIOCHIMIE 180 (2021) : 68-78 . |
| APA | Zaynab, Madiha , Pan, Dezhuo , Fatima, Mahpara , Sharif, Yasir , Chen, Shipin , Chen, Wei . Proteomics analysis of Cyclobalanopsis gilva provides new insights of low seed germination . | BIOCHIMIE , 2021 , 180 , 68-78 . |
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Laver is one of the major arsenic contributors to human diets. The study on metabolic and residual characteristic of each arsenic species contained in laver is important to scientifically assess the intake risk of arsenic in the laver. The metabolic and residual characteristic of main arsenic species in laver, namely arsenate [As(V)], dimethylarsinic acid [DMA(V)] and two arsenosugars, was investigated by mouse experiments in this study. The results showed that the intake of higher-dose laver did not lead to a notable increase of As(V) concentration in mouse muscle/organs and feces. In contrast, DMA(V) excretion in feces and DMA(V) residue in muscle/organs showed a close correlation with laver-dose intake. Most DMAsSugarMethoxy was translated into other arsenic species and then was together excreted out via mouse feces; two dominant arsenic species, arsenosugar DMAsSugarMethoxy and DMAsSugarPhosphate, were not detected in mouse muscle/organs after 20-Day or 30-Day feeding whether in lower-dose laver groups containing 1/36 (mass ratio) of the laver in mouse feed or higher-dose laver groups contain-ing 1/6 (mass ratio) of the laver in mouse feed. About 65-77% of total arsenic digested by mouse was excreted out via feces; only 0.12-0.78% of it was accumulated in mouse organs/muscle. The results of this study provided valuable knowledge for comprehending the stability and metabolic characteristics of different arsenic species from Fujian laver in vivo, also for more scientifically assessing the intake risk of arsenic in laver. (c) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Arsenic Arsenic Arsenosugars Arsenosugars Edible seaweed Edible seaweed Intake risk Intake risk Metabolism Metabolism
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| GB/T 7714 | Wu, YongChen , Zhang, Huang , Wang, KaiTeng et al. Metabolic and residual characteristic of different arsenic species contained in laver during mouse digestion [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 793 . |
| MLA | Wu, YongChen et al. "Metabolic and residual characteristic of different arsenic species contained in laver during mouse digestion" . | SCIENCE OF THE TOTAL ENVIRONMENT 793 (2021) . |
| APA | Wu, YongChen , Zhang, Huang , Wang, KaiTeng , Chen, Wei , Liu, ZhiFeng , Chen, Lian et al. Metabolic and residual characteristic of different arsenic species contained in laver during mouse digestion . | SCIENCE OF THE TOTAL ENVIRONMENT , 2021 , 793 . |
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糖酵解是细胞的主要能量来源,对维持细胞的pH稳态平衡至关重要。然而,尚未清楚糖酵解酶对pH稳态平衡的影响。本研究以拟南芥(Arabidopsis thaiana)胞质磷酸甘油酸激酶(cytosolic phosphoglycerate kinase, PGKc)为研究对象,对PGKc响应外界pH胁迫的机制进行了研究。结果表明:pgkc突变体的根表现出对pH敏感的表型,其表型可被回补。pgkc分泌H+的能力减弱,液泡碱性化,影响了根系细胞的pH稳态平衡。pgkc根中,参与pH生物化学调控机制的主要有机酸含量未受显著影响,保持在稳定的水平。而ATP酶(ATPase)活性,特别是液泡ATP酶(V-ATPase)活性显著降低。说明PGKc主要通过生物物理调控机制来维持细胞pH稳态平衡。综上,本研究揭示了PGKc通过生物物理机制调控细胞pH稳态平衡,为研究植物糖酵解酶调节pH稳态平衡的作用提供基础和新的思路。
Keyword ：
pH稳态平衡 pH稳态平衡 拟南芥 拟南芥 液泡ATP酶 液泡ATP酶 糖酵解 糖酵解 胞质磷酸甘油酸激酶(PGKc) 胞质磷酸甘油酸激酶(PGKc)
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| GB/T 7714 | 王晓国 , 龚萍萍 , 厉苏宁 et al. 拟南芥胞质磷酸甘油酸激酶(PGKc)对细胞pH稳态平衡的影响 [J]. | 植物生理学报 , 2021 , 57 (10) : 1907-1914 . |
| MLA | 王晓国 et al. "拟南芥胞质磷酸甘油酸激酶(PGKc)对细胞pH稳态平衡的影响" . | 植物生理学报 57 . 10 (2021) : 1907-1914 . |
| APA | 王晓国 , 龚萍萍 , 厉苏宁 , 陈伟 , 李瑞姿 , 卞江虎 et al. 拟南芥胞质磷酸甘油酸激酶(PGKc)对细胞pH稳态平衡的影响 . | 植物生理学报 , 2021 , 57 (10) , 1907-1914 . |
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