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学者姓名：柯玉琴
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Abstract ：
BackgroundBoron (B) is an essential micronutrient for plants. Inappropriate B supply detrimentally affects the productivity of numerous crops. Understanding of the molecular responses of plants to different B supply levels would be of significance in crop improvement and cultivation practices to deal with the problem.ResultsWe conducted a comprehensive analysis of the transcriptome and proteome of tobacco seedlings to investigate the expression changes of genes/proteins in response to different B supply levels, with a particular focus on B deficiency. The global gene and protein expression profiles revealed the potential mechanisms involved in the responses of tobacco to B deficiency, including up-regulation of the NIP5;1-BORs module, complex regulation of genes/proteins related to cell wall metabolism, and up-regulation of the antioxidant machinery.ConclusionOur results demonstrated that B deficiency caused severe morphological and physiological disorders in tobacco seedlings, and revealed dynamic expression changes of tobacco genes/proteins in response to different B supply levels, especially to B deficiency, thus offering valuable insights into the molecular responses of tobacco to B deficiency.
Keyword ：
Antioxidant machinery Antioxidant machinery BOR BOR Boron Boron Cell wall-related genes Cell wall-related genes NIP NIP Proteome analysis Proteome analysis Tobacco Tobacco Transcriptome analysis Transcriptome analysis
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| GB/T 7714 | Lin, Jinbin , Zheng, Xiangli , Xia, Jing et al. Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency [J]. | BMC PLANT BIOLOGY , 2024 , 24 (1) . |
| MLA | Lin, Jinbin et al. "Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency" . | BMC PLANT BIOLOGY 24 . 1 (2024) . |
| APA | Lin, Jinbin , Zheng, Xiangli , Xia, Jing , Xie, Rongrong , Gao, Jingjuan , Ye, Rongrong et al. Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency . | BMC PLANT BIOLOGY , 2024 , 24 (1) . |
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为探索烤烟合理的施镁方式，以不施镁肥为对照，设置根部基施、根部追施、根部基施+根部追施、根部基施+叶面追施4个施镁处理，比较不同施镁方式烤烟生长后期生理代谢差异。结果表明：施镁处理中，根部追施处理潜在光合效率、最大光化合效率显著大于根部基施处理及根部基施+根部追施处理。根部追施及根部基施+叶面追施处理蔗糖磷酸合成酶、硝酸还原酶、谷氨酰胺合成酶、超氧化物歧化酶、过氧化氢酶、过氧化物酶活性显著高于根部基施处理，增幅分别为12.29%～32.20%、23.38%～53.90%、11.43%～14.29%、19.86%～22.70%、28.30%～32.08%、17.15%～24.65%，丙二醛、H
Keyword ：
光合作用 光合作用 抗氧化酶活性 抗氧化酶活性 施镁方式 施镁方式 烤烟 烤烟 碳氮代谢 碳氮代谢
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| GB/T 7714 | 王月敏 , 谢榕榕 , 柯玉琴 et al. 不同施镁方式对烤烟生长后期生理代谢的影响 [J]. | 中国烟草科学 , 2024 , 45 (05) : 33-40 . |
| MLA | 王月敏 et al. "不同施镁方式对烤烟生长后期生理代谢的影响" . | 中国烟草科学 45 . 05 (2024) : 33-40 . |
| APA | 王月敏 , 谢榕榕 , 柯玉琴 , 郑朝元 , 郭金平 , 李文卿 . 不同施镁方式对烤烟生长后期生理代谢的影响 . | 中国烟草科学 , 2024 , 45 (05) , 33-40 . |
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为研究提升烟叶品质的最佳钾镁比例，通过田间随机区组试验，以农户常规施肥（T
Keyword ：
活性氧代谢 活性氧代谢 激素 激素 烤烟 烤烟 生物量 生物量 钾镁互作 钾镁互作
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| GB/T 7714 | 朱晨宇 , 谢榕榕 , 王月敏 et al. 钾镁肥优化对烤烟后期活性氧代谢和激素含量的影响 [J]. | 中国农学通报 , 2024 , 40 (25) : 24-29 . |
| MLA | 朱晨宇 et al. "钾镁肥优化对烤烟后期活性氧代谢和激素含量的影响" . | 中国农学通报 40 . 25 (2024) : 24-29 . |
| APA | 朱晨宇 , 谢榕榕 , 王月敏 , 徐志豪 , 柯玉琴 , 郑朝元 et al. 钾镁肥优化对烤烟后期活性氧代谢和激素含量的影响 . | 中国农学通报 , 2024 , 40 (25) , 24-29 . |
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High light (HL) significantly impacts plant photosynthesis. This study investigated the effects of different magnesium (Mg) levels (0, 1, 2, and 5 mol Mg plant-1; HMg0, HMg1, HMg2, and HMg5) on tobacco (Nicotiana tabacum L. cv. Cuibi No. 1) under HL (1500 mu mol m-2 s-1), aiming to understand the role of Mg in mitigating the impact of HL on photosynthesis and carbon-nitrogen metabolism. Plants treated with 1 mol Mg plant-1 under 750 mu mol m-2 s-1 light conditions served as the control. HL led to a reduced chlorophyll (Chl) content and inhibited the maximum photosynthetic rate (Pmax). It also decreased energy involved in photosynthetic electron transfer (ET) and electron flux to reduction end-electron acceptors at the photosystems I (PSI) acceptor side (RE) and caused photosynthetic system damage. H2O2 accumulation exacerbated membrane lipid peroxidation damage, disrupting carbon and nitrogen metabolism, and inducing antioxidant enzyme activity. HMg2 increased Chl content, stomatal conductance, intercellular CO2 concentration, and the net photosynthetic rate compared to HMg0. It enhanced ET efficiency, PSI and PSII functionality, reduced dissipated energy flux (DI), and minimized photosynthesis damage. Conversely, excessive Mg application (HMg5) decreased Pmax and PSII activity, increasing DI. Adequate Mg supply alleviated HL's detrimental effects by enhancing Chl content and ET and RE efficiency.
Keyword ：
carbon and nitrogen metabolism carbon and nitrogen metabolism high light intensity high light intensity magnesium deficiency magnesium deficiency photosynthetic fluorescence characteristic photosynthetic fluorescence characteristic
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| GB/T 7714 | Xie, Rongrong , Gao, Jingjuan , Yang, Zongfeng et al. Improving High Light Tolerance of Tobacco Plants: Adequate Magnesium Supply Enhances Photosynthetic Performance [J]. | AGRONOMY-BASEL , 2024 , 14 (7) . |
| MLA | Xie, Rongrong et al. "Improving High Light Tolerance of Tobacco Plants: Adequate Magnesium Supply Enhances Photosynthetic Performance" . | AGRONOMY-BASEL 14 . 7 (2024) . |
| APA | Xie, Rongrong , Gao, Jingjuan , Yang, Zongfeng , Wang, Yuemin , Tong, Liangyuan , Ke, Yuqin et al. Improving High Light Tolerance of Tobacco Plants: Adequate Magnesium Supply Enhances Photosynthetic Performance . | AGRONOMY-BASEL , 2024 , 14 (7) . |
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Introduction: Magnesium (Mg) is a crucial macronutrient for plants. Understanding the molecular responses of plants to different levels of Mg supply is important for improving cultivation practices and breeding new varieties with efficient Mg utilization. Methods: In this study, we conducted a comprehensive transcriptome analysis on tobacco (Nicotiana tabacum L.) seedling leaves to investigate changes in gene expression in response to different levels of Mg supply, including Mg-deficient, 1/4-normal Mg, normal Mg, and 4x-normal Mg, with a particular focus on Mg deficiency at 5, 15 and 25 days after treatment (DAT), respectively. Results: A total of 11,267 differentially expressed genes (DEGs) were identified in the Mg-deficient, 1/4-normal Mg, and/or 4x-normal Mg seedlings compared to the normal Mg seedlings. The global gene expression profiles revealed potential mechanisms involved in the response to Mg deficiency in tobacco leaves, including down-regulation of genes-two DEGs encoding mitochondria-localized NtMGT7 and NtMGT9 homologs, and one DEG encoding a tonoplast-localized NtMHX1 homolog-associated with Mg trafficking from the cytosol to mitochondria and vacuoles, decreased expression of genes linked to photosynthesis and carbon fixation at later stages, and up-regulation of genes related to antioxidant defenses, such as NtPODs, NtPrxs, and NtGSTs. Discussion: Our findings provide new insights into the molecular mechanisms underlying how tobacco responds to Mg deficiency.
Keyword ：
antioxidant response antioxidant response magnesium magnesium Mg2+/H+ exchanger Mg2+/H+ exchanger Mg2+ transporter Mg2+ transporter photosynthesis photosynthesis tobacco tobacco transcriptome analysis transcriptome analysis
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| GB/T 7714 | Liang, Tingmin , Lin, Jinbin , Wu, Shengxin et al. Integrative transcriptomic analysis reveals the molecular responses of tobacco to magnesium deficiency [J]. | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
| MLA | Liang, Tingmin et al. "Integrative transcriptomic analysis reveals the molecular responses of tobacco to magnesium deficiency" . | FRONTIERS IN PLANT SCIENCE 15 (2024) . |
| APA | Liang, Tingmin , Lin, Jinbin , Wu, Shengxin , Ye, Rongrong , Qu, Mengyu , Xie, Rongrong et al. Integrative transcriptomic analysis reveals the molecular responses of tobacco to magnesium deficiency . | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
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为了完善烟稻轮作烟区养分管理和促进烤烟生长后期烟叶“养熟”措施优化，通过大田试验，在水稻季和烤烟季设置T1（烤烟季水稻季都施用纯化肥）、T2（烤烟季化肥+饼肥，水稻季纯化肥）、T3（烤烟季纯化肥，水稻季化肥+饼肥）、T4（烤烟季水稻季都施用化肥+饼肥）4种施肥处理，研究烟稻轮作烟田不同作物季施用有机肥对烟株生长后期光合特性、荧光特性以及碳氮代谢关键酶活性的影响。结果表明，施用饼肥处理各项指标均比2季都施用纯化肥处理好，饼肥在烤烟当季施用比水稻季施用对烟株生理效应更好，烟稻2季均施用化肥+饼肥处理对烟株生理效应最好，T4处理总叶绿素含量较T2、T3、T1处理分别增加15.0%、26.6%、45.3%,T4处理净光合速率较T2、T3、T1处理分别增加13.3%、36.1%、66.0%。烟稻2季均化肥饼肥配施能更显著的提高光合速率，促进光合产物的转化以及维持氮代谢水平，保证烤烟生长后期烟株体内各种代谢过程的顺利进行，更有利于烟叶在田间“养熟”和优质烟叶的品质形成，从而提高烟农收益。
Keyword ：
光合作用 光合作用 有机肥 有机肥 烟稻轮作 烟稻轮作 碳氮代谢 碳氮代谢 荧光特性 荧光特性
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| GB/T 7714 | 高静娟 , 朱晨宇 , 柯玉琴 et al. 烟稻轮作条件下有机肥施用时期对烤烟碳氮代谢的影响 [J]. | 中国农业科技导报 , 2023 , 25 (09) : 157-165 . |
| MLA | 高静娟 et al. "烟稻轮作条件下有机肥施用时期对烤烟碳氮代谢的影响" . | 中国农业科技导报 25 . 09 (2023) : 157-165 . |
| APA | 高静娟 , 朱晨宇 , 柯玉琴 , 郑朝元 , 李春英 , 李文卿 . 烟稻轮作条件下有机肥施用时期对烤烟碳氮代谢的影响 . | 中国农业科技导报 , 2023 , 25 (09) , 157-165 . |
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为了解不同形态氮素与不同镁浓度对烤烟早期光合荧光特性及生长的影响，以翠碧一号烤烟为材料，采用水培试验方法，在温度较低、光照较弱条件下设置3种氮素处理和两个镁水平，分析不同处理烟株光合荧光特性及生长的差异。结果表明，加镁条件下铵、硝态氮各50%处理烟株的生物量、最大净光合速率（P_(max)）、表观量子效率（AQY）和光系统Ⅱ(PSⅡ)与光系统Ⅰ(PSⅠ)及系统间电子传递链的整体功能活性指数（PI _(total)）均最高，其中生物量相较于纯硝态氮且加镁处理提高21.7%，相较于纯铵态氮加镁处理提高96.0%；不加镁条件下全硝态氮处理P_(max)和AQY高于铵、硝态氮各50%处理且显著高于全铵态氮处理。纯硝态氮处理和铵、硝态氮各50%处理加镁后显著提高各项指标，但加镁对全氨态氮处理没有显著性影响。可见，在温度较低、光照较弱条件下，铵、硝各50%且加镁处理更有利于烤烟早期的生长发育。
Keyword ：
光合特性 光合特性 氮素形态 氮素形态 烤烟 烤烟 荧光特性 荧光特性 镁 镁
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| GB/T 7714 | 高静娟 , 柯玉琴 , 谢榕榕 et al. 不同形态氮素与不同镁浓度供应对烤烟光合荧光特性的影响 [J]. | 中国烟草科学 , 2023 , 44 (04) : 17-24 . |
| MLA | 高静娟 et al. "不同形态氮素与不同镁浓度供应对烤烟光合荧光特性的影响" . | 中国烟草科学 44 . 04 (2023) : 17-24 . |
| APA | 高静娟 , 柯玉琴 , 谢榕榕 , 朱晨宇 , 郑朝元 , 李文卿 et al. 不同形态氮素与不同镁浓度供应对烤烟光合荧光特性的影响 . | 中国烟草科学 , 2023 , 44 (04) , 17-24 . |
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Zinc (Zn) is an essential micronutrient for plants. Adequate regulation of Zn uptake, transport and distribution, and adaptation to Zn-deficiency stress or Zn-excess toxicity are crucial for plant growth and development. However, little has been done to understand the molecular responses of plants toward different Zn supply levels. In the present study, we investigated the growth and physiological responses of tobacco seedlings grown under Zn-completely deficient, Zn-limiting, Zn-normal, and Zn-4-fold sufficient conditions, respectively, and demonstrated that Zn deficiency/limitation caused oxidative stress and impaired growth of tobacco plants. Combined transcriptome and proteome analysis revealed up-regulation of genes/proteins associated with Zn uptake and distribution, including ZIPs, NAS3s, and HMA1s, and up-regulation of genes/proteins involved in regulation of oxidative stress, including SODs, APX1s, GPX6, and GSTs in tobacco seedlings in response to Zn deficiency/ limitation, suggesting that tobacco possessed mechanisms to regulate Zn homeostasis primarily through upregulation of the ZIPs-NAS3s module, and to alleviate Zn deficiency/limitation-induced oxidative stress through activation of the antioxidant machinery. Our results provide novel insights into the adaptive mechanisms of tobacco in response to different Zn supplies, and would lay a theoretical foundation for development of varieties of tobacco or its relatives with high tolerance to Zn-deficiency.
Keyword ：
Antioxidant regulation Antioxidant regulation Proteome analysis Proteome analysis Tobacco Tobacco Transcriptome analysis Transcriptome analysis Zinc Zinc ZIP transporters ZIP transporters
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| GB/T 7714 | Lu, Jianjun , Ye, Rongrong , Qu, Mengyu et al. Combined transcriptome and proteome analysis revealed the molecular regulation mechanisms of zinc homeostasis and antioxidant machinery in tobacco in response to different zinc supplies [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2023 , 202 . |
| MLA | Lu, Jianjun et al. "Combined transcriptome and proteome analysis revealed the molecular regulation mechanisms of zinc homeostasis and antioxidant machinery in tobacco in response to different zinc supplies" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 202 (2023) . |
| APA | Lu, Jianjun , Ye, Rongrong , Qu, Mengyu , Wang, Yuemin , Liang, Tingmin , Lin, Jinbin et al. Combined transcriptome and proteome analysis revealed the molecular regulation mechanisms of zinc homeostasis and antioxidant machinery in tobacco in response to different zinc supplies . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2023 , 202 . |
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为探究叶面喷施微肥对烟叶成熟的影响，提高烟叶适烤性，以‘翠碧一号’为试验材料，在连续10年纯化肥定位施肥烟田，于烟株打顶后10、20天时喷施不同中微量元素，测定其对烟叶生理代谢的影响。叶面喷施不同中微量元素后，叶绿素a、叶绿素b和叶绿素总量均有较明显上升，叶片光合速率、蒸腾速率和气孔导度明显提高，荧光参数中Fo和Fo/Fm显著下降，Fm、Fv、Fv/Fm和Fv/Fo显著上升；硝酸还原酶活性显著提升；细胞膜相对透性显著降低；过氧化氢酶和超氧化物歧化酶均能保持较高的活性，而丙二醛含量显著下降。表明在长期纯施化肥烟田的烤烟生长后期喷施中微量元素有利于提高细胞膜结构稳定性，减轻膜脂过氧化程度，维持光合结构的稳定性，减少叶绿体色素的降解，提高光能转化效率，减缓活性氧伤害。喷施微肥有利于提高烟叶光合作用能力，缓解烟株叶片伤害与衰老，提高上部烟叶的适烤性。
Keyword ：
中微量元素 中微量元素 光合作用 光合作用 定位施肥 定位施肥 烤烟 烤烟 生理代谢 生理代谢
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| GB/T 7714 | 王月敏 , 柯玉琴 , 谢榕榕 et al. 喷施微肥对定位施肥烟株成熟期生理代谢的影响 [J]. | 中国农学通报 , 2022 , 38 (31) : 24-30 . |
| MLA | 王月敏 et al. "喷施微肥对定位施肥烟株成熟期生理代谢的影响" . | 中国农学通报 38 . 31 (2022) : 24-30 . |
| APA | 王月敏 , 柯玉琴 , 谢榕榕 , 李春英 , 李文卿 . 喷施微肥对定位施肥烟株成熟期生理代谢的影响 . | 中国农学通报 , 2022 , 38 (31) , 24-30 . |
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以翠碧一号为试验材料，于福建省烟科所宦溪科研基地布置田间随机区组试验，共设0(T_1,CK)、19.5(T_2)、39.0(T_3)、58.5(T_4)、78.0(T_5) kg/hm~2 MgO等5个镁肥用量。结果表明：全生育期以对照的烤烟根系和地上部的氮、磷、钾、镁、钙累积量最小；T_5处理的烤烟根系的镁、钾累积量最大，分别为1.60和9.91 kg/hm~2，分别比对照增加了26.98%和8.91%;T_3处理的烟株根系的氮累积量最高，为9.92 kg/hm~2，比对照增加了40.5%；磷和钙累积量在移栽后150 d达到最高，分别为10.46和4.77 kg/hm~2，分别比对照增加了29.1%和4.6%。移栽后63 d，烟株地上部的氮、磷累积量均在T_4处理下达到最高，分别为4.13和2.48 kg/hm~2，分别比对照增加17.55%和10.33%；全生育期烟株地上部的钾累积量在T_4处理下最高，为100.04 kg/hm~2，比对照增加4.80%；烟株地上部的镁在移栽后105 d,T_4处理下达到最高，为8.53 kg/hm~2，比对照提高了69.06%;T_5处理下，烟株地上部的钙累积量在移栽后150 d达到最高，为38.48 kg/hm~2，比对照增加1.42%。适宜的镁肥投入可以促进烟株氮、磷、钾、钙、镁的累积；高镁施用量抑制了烟株地上部的钾累积，促进了根系对钾的累积。因此，福建烤烟生产推荐镁肥投入量为40～55 kg/hm~2 MgO，增施镁肥可有效改善烟株对养分的吸收和累积。
Keyword ：
产量 产量 养分累积 养分累积 烤烟 烤烟 镁肥 镁肥
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| GB/T 7714 | 李健 , 孙爱华 , 郭秦羽 et al. 不同施镁量对烟株养分累积规律的影响 [J]. | 江西农业学报 , 2022 , 34 (11) : 1-12 . |
| MLA | 李健 et al. "不同施镁量对烟株养分累积规律的影响" . | 江西农业学报 34 . 11 (2022) : 1-12 . |
| APA | 李健 , 孙爱华 , 郭秦羽 , 李文卿 , 柯玉琴 , 李春英 et al. 不同施镁量对烟株养分累积规律的影响 . | 江西农业学报 , 2022 , 34 (11) , 1-12 . |
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