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学者姓名：林占熺
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Abstract ：
Waterlogging, or excessive accumulation of water in the soil, poses significant stress to riparian ecosystems and agroforestry, especially with increasing global rainfall. Cenchrus fungigraminus is a vital agricultural resource, biomaterial, and super-energy plant with high resistance and adaptability. This study examined its morphological and physiological responses under root and above-ground waterlogging for up to 30 days. Results showed that waterlogging significantly inhibited growth, reducing membrane permeability, and root activity, and accelerating leaf senescence (P < 0.05). Despite this, C. fungigraminus achieved 100 % survival after 30 days of waterlogging. The plant adapted to the hypoxic environment by enhancing oxygen channels through cortex cell loosening, lysigenous tissue formation, and adventitious root development. It also activated defense mechanisms, increasing the activities of antioxidant enzymes (SOD, POD, and CAT), levels of non-enzymatic antioxidants (AsA and GSH), osmotic regulators (SS, SP, and Pro), and anaerobic respiratory enzymes (PDC, ADH, and LDH), and hormones (ABA, IAA, GA, and ETH). Under two levels of waterlogging depth, the plant initially adopted the LowO2 escape strategy (LOES), but over time, it transitioned to the Low-O2 quiescence strategy (LOQS), while still retaining some features of the LOES. Our results revealed that C. fungigraminus demonstrates strong adaptability to waterlogging, especially in response to root waterlogging. By employing anatomical adjustments and exceptional cellular defense mechanisms, the species effectively mitigates damage, establishing itself as an excellent forage grass for slope protection under waterlogged conditions. These results offer valuable guidance for selecting waterlogging-tolerant species to restore and rehabilitate degraded riparian ecosystems in the Yellow River Basin, optimize land use in waterlogging-prone areas, and advance the genetic improvement of waterlogging tolerance in other forage varieties.
Keyword ：
Anatomy Anatomy Cenchrus fungigraminus Cenchrus fungigraminus Growth strategy Growth strategy Morphology Morphology Physiology Physiology Waterlogging Waterlogging
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| GB/T 7714 | Song, Simeng , Zhou, Hengyu , Luo, Yuan et al. Effects of waterlogging at different duration on growth and physiological characteristics of Cenchrus fungigraminus [J]. | ENVIRONMENTAL AND EXPERIMENTAL BOTANY , 2025 , 231 . |
| MLA | Song, Simeng et al. "Effects of waterlogging at different duration on growth and physiological characteristics of Cenchrus fungigraminus" . | ENVIRONMENTAL AND EXPERIMENTAL BOTANY 231 (2025) . |
| APA | Song, Simeng , Zhou, Hengyu , Luo, Yuan , Yu, Shikui , Su, Dewei , Zheng, Dan et al. Effects of waterlogging at different duration on growth and physiological characteristics of Cenchrus fungigraminus . | ENVIRONMENTAL AND EXPERIMENTAL BOTANY , 2025 , 231 . |
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Ganoderma polysaccharides (peptides) are essential bio-macromoleculars that determine the quality of Ganoderma. Their molecular weight,distribution and content are influenced by factors such as origin, strain, cultivation conditions, harvesting period and processing methods. In this study, we established a molecular weight specific chromatogram using high performance gel permeation chromatography (HPGPC) for Ganoderma glycopeptide and assessed its potential applications. The results revealed consistent molecular weight distributions across different cultivation conditions, with 6-8 characteristic peaks showing similarity values exceeding 0.93. The average molecular weight of common peaks was highest during the budding stage and lowest in the mature stage. Ganoderma lucidum (GL) exhibited higher molecular weights compared to Ganoderma sinense (GS). Moreover, both molecular weight and Ganoderma glycopeptides decreased with prolonged growth periods. The content of GL-PPSQ2 was significantly higher in the budding (1.63 +/- 0.15%) and cap-opening stages (1.51 +/- 0.12%) compared to the mature stage (1.31 +/- 0.05%). Chemometrics analysis revealed distinct molecular weight profiles for different growth stages and varieties. Additionally, the HPGPC method was successfully applied to detect adulteration in Ganoderma extracts, identifying excipients such as maltodextrin and dextran. This study underscores the importance of molecular weight distribution and glycopeptide content as key quality control indicators, providing a rapid and reliable tool for ensuring the authenticity and quality of Ganoderma extracts in the market.
Keyword ：
Adulteration Adulteration Ganoderma Ganoderma Ganoderma extracts Ganoderma extracts Gel permeation chromatography Gel permeation chromatography Glycopeptide Glycopeptide Molecular weight specific chromatogram Molecular weight specific chromatogram
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| GB/T 7714 | Luo, Hongjian , Lin, Shuqian , Lin, Zhanxi et al. Establishment of molecular weight specific chromatogram of Ganoderma glycopeptide and its application in extract [J]. | FOOD BIOSCIENCE , 2025 , 66 . |
| MLA | Luo, Hongjian et al. "Establishment of molecular weight specific chromatogram of Ganoderma glycopeptide and its application in extract" . | FOOD BIOSCIENCE 66 (2025) . |
| APA | Luo, Hongjian , Lin, Shuqian , Lin, Zhanxi , Lu, Haining , Ma, Ang , Lin, Dongmei . Establishment of molecular weight specific chromatogram of Ganoderma glycopeptide and its application in extract . | FOOD BIOSCIENCE , 2025 , 66 . |
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本发明提出了一种利用植物‑微生物联合无客土修复煤矸石区的方法，旨在通过植物的种植及其生物特性，与微生物通过根系分泌物—微生物代谢的循环，形成自维持修复系统，有效改善煤矸石区生态环境，促进植被恢复，减少淋溶水污染。该方法充分利用了菌草的快速生长、抗逆性强、生物量大以及土壤改良效果好等特性，通过多次刈割平铺，生产大量枯落物，进而形成类土壤基质，改善煤矸石区贫瘠土壤结构，为后续植物群落演替创造条件；同时搭配爬藤植物攀援特性可快速形成绿色覆盖，改善煤矸石区的视觉污染，提升矿区景观。此方法为煤矸石区治理提供了新的思路和技术手段。
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| GB/T 7714 | 林冬梅 , 郑丹 , 林占熺 et al. 一种利用植物-微生物联合无客土修复煤矸石区的方法 : CN202510686411.9[P]. | 2025-05-27 . |
| MLA | 林冬梅 et al. "一种利用植物-微生物联合无客土修复煤矸石区的方法" : CN202510686411.9. | 2025-05-27 . |
| APA | 林冬梅 , 郑丹 , 林占熺 , 苏德伟 , 林辉 , 张明阳 . 一种利用植物-微生物联合无客土修复煤矸石区的方法 : CN202510686411.9. | 2025-05-27 . |
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本发明涉及一种云芝新菌种及其栽培方法。所述云芝新菌种为云芝（Trametes versicolor）TvJC209，其是经组织分离纯化、紫外诱变及定向筛选获得，已于2023年6月19日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 20231064，保藏地址为中国.武汉.武汉大学该菌种。该菌种的ITS序列与其在NCBI数据库中相似度最高的Trametes versicolor voucher ACAD21091F（OL741530.1）存在1个碱基的差异，A→G；该菌种可以多种干或鲜巨菌草、芦竹等菌草作为培养基主要原料进行栽培；该菌种子实体多糖含量达6％、水溶浸出物含量达22％、三萜含量达0.42％、生物学转化率达20％。该菌种的栽培方法包括母种制备、原种制备、栽培及出芝管理。本发明的有益效果是：可以使用菌草这种绿色有机可持续性的原材料栽培云芝，提供高品质云芝。
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| GB/T 7714 | 林冬梅 , 朱敏 , 张煜隆 et al. 一种云芝新菌种及其菌草栽培方法 : CN202510513082.8[P]. | 2025-04-23 . |
| MLA | 林冬梅 et al. "一种云芝新菌种及其菌草栽培方法" : CN202510513082.8. | 2025-04-23 . |
| APA | 林冬梅 , 朱敏 , 张煜隆 , 李治君 , 谢明君 , 林占熺 et al. 一种云芝新菌种及其菌草栽培方法 : CN202510513082.8. | 2025-04-23 . |
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本发明属于生态治理技术领域，涉及一种江河岸线菌草生态防护屏障及其构建方法。通过建立迎风坡面菌草防风固沙（土）带、背风坡面菌草阻沙（土）入河复合草篱带和岸线斜坡面菌草生物防护堤组成的菌草岸线生态防护屏障。本申请提供的江河岸线菌草生态防护屏障及其构建方法是一种投入省、见效快的生物措施，形成有效的江河岸线菌草生态防护屏障，达到快速防风固沙（土）、阻沙（土）入河、提高物种多样性的效果。同时，本申请提供的方案实现了生态效益和经济效益共赢，枯水季，种植的菌草可留茬收割，作为食药用菌的培养料、畜禽饲料、生物能源、生物质材料等，提高经济效益；留茬的菌草防风固沙（土）、阻沙（土）入河，继续发挥其生态价值。
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| GB/T 7714 | 林冬梅 , 林占熺 , 罗宗志 et al. 一种江河岸线菌草生态防护屏障及其构建方法 : CN2024108813W[P]. | 2024-07-31 . |
| MLA | 林冬梅 et al. "一种江河岸线菌草生态防护屏障及其构建方法" : CN2024108813W. | 2024-07-31 . |
| APA | 林冬梅 , 林占熺 , 罗宗志 , 余世葵 , 郑丹 . 一种江河岸线菌草生态防护屏障及其构建方法 : CN2024108813W. | 2024-07-31 . |
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In this study, a comprehensive identification of protein glycosylation of G. lucidum polysaccharide peptide (GL-PPSQ(2)) was investigated. With ZIC-HILIC enrichment, intact Nand O-glycopeptides were analyzed by higher-energy collisional dissociation (HCD) fragmentation on nanoRPLC-ESI-MS/MS. A total of 48 proteins and 62 peptides were identified, including 57 unique peptide segments. Glycoproteomic profiling using pGlyco revealed 42 intact N-glycopeptides from 35 N-glycosites of 32 N-glycoproteins, whereas GPSeeker identified four intact Nglycopeptides with regular N-glycan core structures. For O-glycosylation, pGlyco annotated 338 intact O-glycopeptides from 287 O-glycosites of 160 O-glycoproteins, revealing a marked predominance and heterogeneity of O-glycosylation. These glycopeptides exhibited diverse glycan compositions, including high mannose, complex, and hybrid types, as well as macro-and microheterogeneity. This study presents direct MS analysis of GL-PPSQ(2) glycosylation, enhancing the understanding of glycosylation modifications and glycoprotein research. The findings elucidate the glycosylation patterns and structural characteristics of GL-PPSQ(2), providing scientific evidence for understanding its biological effects and supporting the development of functional foods based on G. lucidum polysaccharide peptide components.
Keyword ：
Ganoderma lucidum polysaccharide peptides Ganoderma lucidum polysaccharide peptides GPSeeker pGlyco GPSeeker pGlyco High-resolution mass spectrometry (HRMS) High-resolution mass spectrometry (HRMS) Intact glycopeptides Intact glycopeptides ZIC-HILIC enrichment ZIC-HILIC enrichment
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| GB/T 7714 | Lin, Dongmei , Wang, Saizhen , Luo, Hongjian et al. Identification of intact Nand O-glycopeptides of Ganoderma lucidum using ZIC-HILIC enrichment and high-resolution mass spectrometry (HRMS) [J]. | MICROCHEMICAL JOURNAL , 2025 , 216 . |
| MLA | Lin, Dongmei et al. "Identification of intact Nand O-glycopeptides of Ganoderma lucidum using ZIC-HILIC enrichment and high-resolution mass spectrometry (HRMS)" . | MICROCHEMICAL JOURNAL 216 (2025) . |
| APA | Lin, Dongmei , Wang, Saizhen , Luo, Hongjian , Wang, Lianfu , Lin, Zhibin , Lin, Shuqian et al. Identification of intact Nand O-glycopeptides of Ganoderma lucidum using ZIC-HILIC enrichment and high-resolution mass spectrometry (HRMS) . | MICROCHEMICAL JOURNAL , 2025 , 216 . |
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为探究复方菌草灵芝颗粒的水提工艺及其质量标准，以浸膏得率和浸膏多糖含量为指标，采用单因素和响应面实验考察提取温度、提取时间、料液比、提取次数和浸泡时间对浸膏得率和浸膏多糖含量的影响；参考2015版《中国药典》和国标检测复方菌草灵芝颗粒的粒度、水分含量、干燥失重、装量差异、可溶性、微生物限量和重金属含量；通过薄层色谱法对复方菌草灵芝颗粒中的菌草灵芝、紫苏和富硒茶原材料进行鉴定；通过高效液相色谱法测定复方菌草灵芝颗粒中单糖和茶多酚含量。结果表明：复方菌草灵芝颗粒的最佳水提工艺为提取温度80℃、超声时间2.5 h、料液比(g/mL)1∶25、提取次数2次、浸泡时间6 h;其颗粒粒度、水分含量、干燥失重、装量差异和可溶性均符合2015版《中国药典》要求，微生物限量和重金属含量均符合国标要求，其中硒平均质量分数为0.058 3 mg/kg;得到的菌草灵芝、紫苏和富硒茶的薄层色谱条带清晰，分离效果好，阴性对照对其测定无干扰；复方菌草灵芝颗粒中甘露糖、鼠李糖、葡萄糖、半乳糖、阿拉伯糖、表没食子儿茶素没食子酸酯、表儿茶素没食子酸酯、儿茶素、没食子酸的质量分数分别为1.310、1.166、8.708、1.265、0.824、5.79、2.58、1.72和1.67 mg/g。以上结果表明优化得到的复方菌草灵芝颗粒水提工艺稳定可靠，颗粒的各项指标均符合相关要求，建立的复方菌草灵芝颗粒水提工艺质量标准科学可行。
Keyword ：
水提工艺 水提工艺 菌草灵芝 菌草灵芝 薄层色谱 薄层色谱 质量标准 质量标准 高效液相色谱 高效液相色谱
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| GB/T 7714 | 张桂清 , 梁志豪 , 刘青 et al. 复方菌草灵芝颗粒的水提工艺及其质量标准研究 [J]. | 陕西师范大学学报(自然科学版) , 2025 , 53 (03) : 66-82 . |
| MLA | 张桂清 et al. "复方菌草灵芝颗粒的水提工艺及其质量标准研究" . | 陕西师范大学学报(自然科学版) 53 . 03 (2025) : 66-82 . |
| APA | 张桂清 , 梁志豪 , 刘青 , 吴小平 , 罗海凌 , 姚俊新 et al. 复方菌草灵芝颗粒的水提工艺及其质量标准研究 . | 陕西师范大学学报(自然科学版) , 2025 , 53 (03) , 66-82 . |
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Pearl millet (Pennisetum glaucum) is a major staple food in arid and semi-arid regions of sub-Saharan Africa, India, and South Asia. However, how epigenetic mechanisms regulate tissue-specific gene expression in this crop remains poorly understood. In this study, we profiled multiple epigenetic features in the young panicles and roots of pearl millet using RNA-seq, ATAC-seq, whole-genome bisulfite sequencing, and ChIP-seq (H3K4me3 and H3K36me3). We identified thousands of genes that were differentially expressed between these two tissues. Root-specific genes were enriched for plant hormone signaling, oxidative phosphorylation, and stress responses. Analysis of chromatin accessibility revealed that root-specific accessible chromatin regions (ACRs) were enriched in binding motifs for stress-responsive transcription factors (e.g., NAC, WRKY), whereas ACRs in young panicles were enriched in motifs for developmental regulators (e.g., AP2/ERF). DNA methylation profiling revealed 25,141 tissue-specific differentially methylated regions, with CHH methylation—rather than CG or CHG methylation—showing the strongest tissue specificity. Promoters of root-specific genes had higher levels of CHH methylation compared to those of young panicle–specific genes, suggesting that the roles of CHH methylation in regulating transcription might be tissue dependent. Notably, promoter-associated H3K4me3 marked panicle-specific genes, whereas root-specific expression was primarily linked to chromatin accessibility, suggesting a transcription factor–mediated regulatory mechanism. Together, our findings highlight the distinct epigenetic frameworks governing tissue-specific gene expression in pearl millet and provide valuable insights for advancing the genetic improvement of this crop. © The Author(s) 2025.
Keyword ：
Alkylation Alkylation Arid regions Arid regions Gene expression regulation Gene expression regulation Methylation Methylation Plants (botany) Plants (botany) Tissue Tissue Tissue engineering Tissue engineering Transcription Transcription Transcription factors Transcription factors
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| GB/T 7714 | Luo, Lin , Qu, Qi , Cao, Mengxue et al. Epigenetic maps of pearl millet reveal a prominent role for CHH methylation in regulating tissue-specific gene expression [J]. | aBIOTECH , 2025 , 6 (3) : 394-410 . |
| MLA | Luo, Lin et al. "Epigenetic maps of pearl millet reveal a prominent role for CHH methylation in regulating tissue-specific gene expression" . | aBIOTECH 6 . 3 (2025) : 394-410 . |
| APA | Luo, Lin , Qu, Qi , Cao, Mengxue , Zhang, Yihui , Sun, Yuanchang , Mao, Fei et al. Epigenetic maps of pearl millet reveal a prominent role for CHH methylation in regulating tissue-specific gene expression . | aBIOTECH , 2025 , 6 (3) , 394-410 . |
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Antler-shaped Ganoderma lucidum (G. lucidum) is cultivated using specialized methods with high content of active ingredients. In this study, we employed a widely-targeted metabolomics to analyze the metabolic regulation in various parts of antler-shaped G. lucidum. Using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), we identified a total of 1016 metabolites and 615 differential metabolites (DMs) across three parts. KEGG pathway analysis found that 353 metabolic pathways were involved in the growth process. The quantitative results revealed that the undifferentiated cap region exhibited the highest content of triterpenes, while the basal region contained higher levels of polysaccharides. Network pharmacology identified 22 key targets from saccharide-related DMs, and 19 key targets from terpenoid-related DMs. These findings demonstrate that antler-shaped G. lucidum can be harvested and processed in a segmented manner to optimize the extraction of bioactive compounds, providing valuable insights for its scientific utilization in nutritional supplements and healthcare.
Keyword ：
Antler-shaped Ganoderma lucidum Antler-shaped Ganoderma lucidum Network pharmacology Network pharmacology Saccharides Saccharides Triterpenes Triterpenes Wide targeted metabolomics Wide targeted metabolomics
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| GB/T 7714 | Luo, Hongjian , Lin, Dongmei , Su, Dewei et al. Wide-target metabolomics and network pharmacology reveal metabolic regulation and targets of bioactive compounds in antler-shaped Ganoderma lucidum [J]. | FOOD BIOSCIENCE , 2025 , 71 . |
| MLA | Luo, Hongjian et al. "Wide-target metabolomics and network pharmacology reveal metabolic regulation and targets of bioactive compounds in antler-shaped Ganoderma lucidum" . | FOOD BIOSCIENCE 71 (2025) . |
| APA | Luo, Hongjian , Lin, Dongmei , Su, Dewei , Luo, Zongzhi , Wang, Lianfu , Lu, Guodong et al. Wide-target metabolomics and network pharmacology reveal metabolic regulation and targets of bioactive compounds in antler-shaped Ganoderma lucidum . | FOOD BIOSCIENCE , 2025 , 71 . |
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本发明涉及一种低温型血红栓菌新菌种TsJC206及其菌草栽培方法。TsJC206其可用一种或多种菌草作为培养基主要原料，如巨菌草、芦竹等，甚至可用巨菌草鲜草、芦竹鲜草等栽培，子实体三萜含量达1.15％、多糖含量达10.82％、生物学转化率达25％；二是为低温型菌种，出芝温度15~20℃，显著低于现有技术报道的出芝温度，补充了血红栓菌的出芝温型；三是其NCBI数据库中ITS序列第182位点的碱基为G，与其在NCBI数据库中相似度最高的OR062414.1在第223位点的碱基A存在差异。所述栽培方法包括母种制备、原种制备、栽培及出芝管理。本发明有益效果：可在15~20℃较低温条件下出芝；可用菌草这种绿色有机可持续性的原材料栽培血红栓菌；可用鲜菌草代替部分培养料，减少干燥培养料的步骤，降低成本。
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| GB/T 7714 | 林冬梅 , 张煜隆 , 谢明君 et al. 一种低温型血红栓菌新菌种及其菌草栽培方法 : CN202510514332.X[P]. | 2025-04-23 . |
| MLA | 林冬梅 et al. "一种低温型血红栓菌新菌种及其菌草栽培方法" : CN202510514332.X. | 2025-04-23 . |
| APA | 林冬梅 , 张煜隆 , 谢明君 , 林占熺 , 李治君 , 罗海凌 et al. 一种低温型血红栓菌新菌种及其菌草栽培方法 : CN202510514332.X. | 2025-04-23 . |
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