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学者姓名:林思祖
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Abstract :
为明确不同外源激素对杉木涩籽发生的影响,以不同类型球果杉木母株(紧包型球果4株、松张型球果5株和反翘型球果3株)为研究对象,采用外部喷施的方法,对不同浓度外源赤霉素(GA_3)、吲哚乙酸(IAA)和玉米素(ZT)对杉木种子涩籽率的影响进行了研究。结果显示,GA_3对杉木涩籽发生有明显的抑制作用,随着喷施GA_3浓度的提高,杉木种子的涩籽率呈现显著下降的趋势(P<0.05);较低浓度(30~90 mg·L~(-1))的IAA可以有效降低杉木种子的涩籽率,但高浓度IAA处理下杉木种子的涩籽率没有显著变化;ZT对杉木种子的涩籽率的影响不显著。不同类型球果对外源激素影响的响应程度不同,GA_3处理下反翘型球果涩籽率降幅最大,松张型球果次之,紧包型球果最小; IAA处理下各类型球果涩籽率未见显著差异; ZT处理下仅在10 mg·L~(-1)浓度时发现反翘型与紧包型球果的涩籽率变化量存在显著差异(P<0.05)。3种外源激素对球果上部种鳞涩籽率的影响较小,对中部和下部种鳞涩籽率有较明显的影响。
Keyword :
外源激素 外源激素 杉木 杉木 涩籽 涩籽 良种生产 良种生产
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| GB/T 7714 | 吴超 , 李佳 , 熊升 et al. 外源激素对杉木涩籽发生的影响 [J]. | 森林与环境学报 , 2023 , 43 (03) : 250-257 . |
| MLA | 吴超 et al. "外源激素对杉木涩籽发生的影响" . | 森林与环境学报 43 . 03 (2023) : 250-257 . |
| APA | 吴超 , 李佳 , 熊升 , 杨安平 , 林思祖 , 陈宇 . 外源激素对杉木涩籽发生的影响 . | 森林与环境学报 , 2023 , 43 (03) , 250-257 . |
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水力结构是植物长期进化过程中所形成的不同形态结构和水分运输策略。以我国南方重要的商品林树种杉木为研究对象,开展不同分枝级、冠层高度及分枝方向的水力结构特征研究。结果表明:杉木水力结构参数变化具有明显的等级效应,导水率和比导率表现为主茎>1级侧枝>2级侧枝,叶比导率和胡伯尔值表现为1级侧枝>2级侧枝。不同分枝级的水力结构参数受冠层高度的影响明显,主茎的导水率和比导率随冠层高度增加而显著下降,主茎的胡伯尔值则随冠层高度增加而显著升高; 1、2级侧枝的导水率和比导率表现为中部冠层最高,1级侧枝的叶比导率随冠层高度的增加而显著下降,2级侧枝的叶比导率表现为中部冠层最高。在不同分枝方向中,1、2级侧枝的导水率、比导率和叶比导率总体表现为南向、西向高于北向和东向。相关分析表明,不同分枝级的导水率与总直径、边材横截面积、叶干重呈极显著正相关(P<0.01),主茎的比导率与上述指标显著正相关(P<0.05),1、2级侧枝的比导率与上述指标呈负相关关系。可见,杉木高级别的枝条直径越小、水分输导能力越差,这种水力等级效应有利于杉木在遭受干旱胁迫时牺牲劣势枝条而优先保障优势枝条的水分供给,是杉木长期进化过程中适应干旱逆境的重要生存策略。
Keyword :
冠层高度 冠层高度 分枝方向 分枝方向 分枝级 分枝级 杉木 杉木 水力结构 水力结构
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| GB/T 7714 | 郑茹萍 , 张马啸 , 吴亚岚 et al. 杉木不同形态特征对水力结构的影响 [J]. | 森林与环境学报 , 2023 , 43 (01) : 17-25 . |
| MLA | 郑茹萍 et al. "杉木不同形态特征对水力结构的影响" . | 森林与环境学报 43 . 01 (2023) : 17-25 . |
| APA | 郑茹萍 , 张马啸 , 吴亚岚 , 周丽丽 , 林思祖 , 李树斌 . 杉木不同形态特征对水力结构的影响 . | 森林与环境学报 , 2023 , 43 (01) , 17-25 . |
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全球变暖导致干旱强度增大、频率增加,开展植物根系的水力功能及解剖结构特征的研究,对揭示植物的耐旱机理、提高其水分利用效率具有重要的理论及实践意义.以3年生杉木[Cunninghamia lanceolata(Lamb.)Hook.]幼树为研究对象,研究不同径级杉木根系(0-2 mm,2-5 mm和5-10 mm)水力功能性状、解剖结构特征及其二者的相关性,结果表明:导水率、管胞直径、水力直径、纹孔膜直径、纹孔口直径、纹孔膜面积、纹孔塞面积随着杉木根系径级的增加而增大,根系比导率、单位质量导水率、管胞密度、木质部密度、水势随根系径级的增加而减小,细根与中根、粗根之间差异均达显著水平(P <0.05).相关分析结果表明,根系的导水率与管胞直径、水力直径、纹孔膜直径、纹孔膜面积和纹孔塞面积呈极显著正相关(P <0.01),根系的比导率、单位质量导水率与管胞密度、木质部密度呈极显著正相关,与纹孔膜直径、纹孔口直径和纹孔膜面积呈极显著负相关.可见,杉木不同径级根系的水分输导状况受管胞结构及纹孔特征的共同影响,粗根和中根具有相对较大的管胞直径、纹孔膜直径、纹孔口直径等解剖结构,使其具有更大的水分输导能力,而细根具有较大的木质部密度、管胞密度、纹孔密度等解剖结构以及较高的水势,这些高效率的输水结构使其水分运输更具安全性.(图6表2参34)
Keyword :
主成分分析 主成分分析 杉木 杉木 根系 根系 水力功能 水力功能 相关性分析 相关性分析 解剖结构 解剖结构
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| GB/T 7714 | 黄小艳 , 邹志广 , 周丽丽 et al. 杉木不同径级根系木质部的水力功能性状及其与解剖结构的关联性 [J]. | 应用与环境生物学报 , 2023 , 29 (03) : 647-653 . |
| MLA | 黄小艳 et al. "杉木不同径级根系木质部的水力功能性状及其与解剖结构的关联性" . | 应用与环境生物学报 29 . 03 (2023) : 647-653 . |
| APA | 黄小艳 , 邹志广 , 周丽丽 , 张马啸 , 林思祖 , 李树斌 . 杉木不同径级根系木质部的水力功能性状及其与解剖结构的关联性 . | 应用与环境生物学报 , 2023 , 29 (03) , 647-653 . |
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Chinese fir (Cunninghamia lanceolata) is the most cultivated timber species in China, with a plantation area of 11 million ha. Due to its extensive geographical distribution, drought stress caused by the spatial and seasonal heterogeneity of precipitation has limited its survival and productivity. To facilitate the breeding of drought-tolerant clones and understand the inter-response mechanisms to drought stress, we screened two drought-tolerant (DT) clones and evaluated their differences in physiological and molecular response to drought. The results showed that the No. 228 clone (high-DT ability) had higher antioxidant enzyme abilities than the No. 026 clone (low-DT ability) under drought stress, e.g., peroxidase (POD), polyphenol oxidase (PPO), superoxide dismutase (SOD), and catalase (CAT). Transcriptome analyses revealed that 6637 genes and 1168 genes were up-regulated in No. 228 and No. 026 under drought stress, respectively, when compared to the control (CK). The genes may participate in response to drought-stimulated signal transduction, water/oxygen-containing compound synthesis, photosynthesis, and transmembrane transport functions. Particularly, under drought stress, 14,213 up-regulated and differentially expressed genes (DEGs) were observed in the No. 228 clone compared with the No. 026 clone, and 4274 up-regulated genes were differentially expressed (15-fold difference). These significant DEGs were involved in plant hormone signal transduction, flavonoid biosynthesis, peroxisomes, and other key pathways related to drought. Interestingly, under drought stress, two Chitinases (ClCHIs) and four POD genes (ClPERs) were induced to express in No. 228, which was consistent with the higher antioxidant enzyme activities in No. 228. A heat map of 49 DEGs revealed that dehydrin family genes, ion binding/transmembrane proteins, auxin receptor proteins, and ethylene-responsive transcription factors were significantly up-regulated under drought stress. The results can enhance our understanding of drought tolerance mechanisms and provide a guideline for screening DT genes and breeding drought-tolerant Chinese fir clones.
Keyword :
Chinese fir Chinese fir DEGs DEGs drought tolerance drought tolerance enzyme activity enzyme activity screening screening transcriptome analysis transcriptome analysis
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| GB/T 7714 | Li, Shubin , Yan, Xinyang , Huang, Xiaoyan et al. Physiological Differences and Transcriptome Analysis Reveal That High Enzyme Activity Significantly Enhances Drought Tolerance in Chinese Fir (Cunninghamia lanceolata) [J]. | FORESTS , 2023 , 14 (5) . |
| MLA | Li, Shubin et al. "Physiological Differences and Transcriptome Analysis Reveal That High Enzyme Activity Significantly Enhances Drought Tolerance in Chinese Fir (Cunninghamia lanceolata)" . | FORESTS 14 . 5 (2023) . |
| APA | Li, Shubin , Yan, Xinyang , Huang, Xiaoyan , Addo-Danso, Shalom Daniel , Lin, Sizu , Zhou, Lili . Physiological Differences and Transcriptome Analysis Reveal That High Enzyme Activity Significantly Enhances Drought Tolerance in Chinese Fir (Cunninghamia lanceolata) . | FORESTS , 2023 , 14 (5) . |
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[目的]比较杉木涩籽形成过程中类黄酮类代谢物的积累特征,为进一步揭示杉木涩籽形成机制提供参考。[方法]以涩籽率分别为24%和90%的杉木家系(L家系和H家系)种子为试材,分别于授粉后70、80、90、100、110 d取样。采用试剂盒、高效液相色谱、荧光定量PCR等方式测定并分析不同家系种子类黄酮生物合成途径关键代谢物含量、酶活性和基因表达量。[结果]H家系种子发育过程中总黄酮含量及花青素、槲皮素、山奈酚、杨梅素和儿茶素等5种类黄酮类代谢物含量明显高于L家系;2个家系间查尔酮合成酶、查尔酮异构酶和二氢黄酮醇4-还原酶等3种酶活性仅在授粉后70 d呈现明显差异;H家系黄酮醇合成酶、无色花青素还原酶的活性水平变化趋势相近,且分别在授粉后80~110 d、70~110 d极显著高于L家系;2个家系种子CHS、CHI、LAR、ANR基因的相对表达量总体随时间变化呈逐渐升高趋势,且H家系低于L家系。[结论]在涩籽发育初期,类黄酮类次生代谢物质出现异常积累。杉木授粉后小孢子异常发育或与涩籽形成有关,涩籽种胚内细胞的应激系统处于激活状态。
Keyword :
代谢物 代谢物 杉木 杉木 涩籽 涩籽 类黄酮 类黄酮 调控基因 调控基因 酶活性 酶活性
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| GB/T 7714 | 吴超 , 熊升 , 武忆寒 et al. 杉木涩籽形成中类黄酮类代谢物的积累特征 [J]. | 亚热带农业研究 , 2022 , 18 (01) : 32-40 . |
| MLA | 吴超 et al. "杉木涩籽形成中类黄酮类代谢物的积累特征" . | 亚热带农业研究 18 . 01 (2022) : 32-40 . |
| APA | 吴超 , 熊升 , 武忆寒 , 林思祖 , 陈宇 . 杉木涩籽形成中类黄酮类代谢物的积累特征 . | 亚热带农业研究 , 2022 , 18 (01) , 32-40 . |
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Anoectochilus roxburghii (Wall.) Lindl (Ar) and Anoectochilus formosanus Hayata (Af) are two species of valuable Chinese herbal medicines that belong to the genus Anoectochilus in Orchidaceae and have often been applied as the Chinese herbal medicine ?Jin Xian Lian?. However, these species exhibit obvious differences in leaf color, and the difference in their medicinal value remains unclear. In this study, the metabolomes of leaf samples of Ar and Af were analyzed by high-performance liquid chromatography-mass spectrometry (LC?MS) in the positive and negative ion modes. The analysis of the leaves of both species in the positive ion mode identified 4013 metabolites enriched in 106 metabolic pathways, and the analysis in the negative ion mode found 1530 metabolites enriched in 98 metabolic pathways. A differential metabolic profiling analysis of the results obtained in positive ion mode found that 816 differentially accumulated metabolites were enriched in 80 metabolic pathways, whereas 297 differentially accumulated metabolites enriched in 55 metabolic pathways were obtained from the analysis in the negative ion mode. The number of flavonoids was higher than those of alkaloids and terpenoids. A quantitative analysis of the differentially accumulated metabolites showed that the most highly upregulated metabolites (Af/Ar) among the top 20 differentially accumulated metabolites in Af were mainly related to amino acid-related metabolic pathways, whereas the most highly downregulated metabolites (Af/Ar) in Af were mainly associated with flavonoid-related metabolic pathways. In addition, the transcriptome sequencing of Ar and Af leaf samples yielded 40.12 Gb of transcriptomic data. The analysis of differentially expressed genes (DEGs) classified 555 metabolism-related DEGs into 23 metabolism-related KEGG pathways, and the top DEGs were involved in the phenylpropanoid biosynthetic pathways, which included up to 138 DEGs associated with the flavonoid metabolism pathway. In addition, significant differences in the flavonoid-related KEGG pathways were found. A comprehensive analysis of the metabolomes and transcriptomes showed that the genes annotated as chalcone synthase (CHS), chalcone isomerase (CHI), flavonol synthase (FLS), caffeoyl-CoA O-methyltransferase (CCOAOMT) and anthocyanidin synthase (ANS) were downregulated in Af leaves compared with Ar leaves, which might account for the downregulation of flavonoid-related metabolites in Af. In summary, both the metabolomic and transcriptomic analyses showed significant differences in flavonoid metabolism between the Ar and Af leaves, and the mechanisms underlying the molecular regulation of these differences were preliminarily analyzed.
Keyword :
Anoectochilus Anoectochilus Differential gene expression Differential gene expression Metabolic differences Metabolic differences Metabolomic analysis Metabolomic analysis Transcriptome sequencing Transcriptome sequencing
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| GB/T 7714 | Chen, Ying , Yao, Limin , Pan, Wangyun et al. An integrated analysis of metabolomic and transcriptomic profiles reveals flavonoid metabolic differences between Anoectochilus roxburghii and Anoectochilus formosanus [J]. | PROCESS BIOCHEMISTRY , 2021 , 100 : 188-198 . |
| MLA | Chen, Ying et al. "An integrated analysis of metabolomic and transcriptomic profiles reveals flavonoid metabolic differences between Anoectochilus roxburghii and Anoectochilus formosanus" . | PROCESS BIOCHEMISTRY 100 (2021) : 188-198 . |
| APA | Chen, Ying , Yao, Limin , Pan, Wangyun , Guo, Beilin , Lin, Sizu , Wei, Yicong . An integrated analysis of metabolomic and transcriptomic profiles reveals flavonoid metabolic differences between Anoectochilus roxburghii and Anoectochilus formosanus . | PROCESS BIOCHEMISTRY , 2021 , 100 , 188-198 . |
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以杉木'洋020'扦插苗和'洋020'组培苗为实验材料,通过盆栽实验,在调查不同埋土深度处理无性系萌蘖情况的基础上,应用酶联免疫吸附法测定了杉木无性系萌蘖高峰期枝叶、基部韧皮部、根尖等不同器官吲哚乙酸、赤霉素、细胞分裂素及脱落酸含量差异.结果表明:组培苗萌蘖能力强于扦插苗;随着埋土深度增加,2个无性系苗萌蘖能力均呈降低的趋势.不同埋土深度处理根尖和枝叶内源激素含量较高;随着培养时间延长,无性系枝叶吲哚乙酸含量呈上升趋势,赤霉素含量呈下降趋势;埋土深度6 cm处理有利于枝叶及根尖脱落酸的积累,埋土深度9 cm处理有利于根尖细胞分裂素的积累;无性系萌蘖与埋土深度呈极显著负相关,与赤霉素含量呈极显著正相关.不同埋土深度对杉木无性萌蘖有一定的影响,杉木无性系萌蘖高峰期均在8月份;不同器官植物内源激素含量是影响埋土深度处理杉木无性系萌蘖的主要影响因子之一.
Keyword :
内源激素 内源激素 埋土 埋土 无性系 无性系 杉木 杉木 萌蘖 萌蘖
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| GB/T 7714 | 张吉玲 , 叶义全 , 黄樱 et al. 不同埋土深度杉木无性系萌蘖及内源激素含量的差异 [J]. | 西南林业大学学报 , 2021 , 41 (5) : 36-41 . |
| MLA | 张吉玲 et al. "不同埋土深度杉木无性系萌蘖及内源激素含量的差异" . | 西南林业大学学报 41 . 5 (2021) : 36-41 . |
| APA | 张吉玲 , 叶义全 , 黄樱 , 李勇 , 郑宏 , 林思祖 et al. 不同埋土深度杉木无性系萌蘖及内源激素含量的差异 . | 西南林业大学学报 , 2021 , 41 (5) , 36-41 . |
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以杉木实生苗为材料,采用单因素试验方法,探究不同浓度6?BA对铝胁迫下杉木幼苗根、茎和叶中抗氧化酶活性的影响情况.结果表明:与CK相比,随着6?BA浓度的增加,杉木幼苗根、茎、叶中的丙二醛含量及抗氧化酶活性呈现出不同的变化规律.其中叶片中的丙二醛含量和过氧化物酶活性最高,其次是茎和根;过氧化氢酶活性在根中最强,其次是茎和叶;多酚氧化酶活性在叶片中最弱,其次是根和茎.因此,6?BA降低了不同组织中丙二醛的含量,提高了过氧化氢酶,多酚氧化酶和过氧化物酶的活性,表明6?BA可以在一定程度上减轻铝对杉木的毒害.
Keyword :
6-BA 6-BA 不同组织 不同组织 抗氧化酶活性 抗氧化酶活性 杉木 杉木 铝胁迫 铝胁迫
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| GB/T 7714 | 吕蒙蒙 , 陈宇 , 林思祖 . 6−BA对铝胁迫杉木幼苗抗氧化酶活性的研究 [J]. | 西南林业大学学报 , 2021 , 41 (5) : 172-177 . |
| MLA | 吕蒙蒙 et al. "6−BA对铝胁迫杉木幼苗抗氧化酶活性的研究" . | 西南林业大学学报 41 . 5 (2021) : 172-177 . |
| APA | 吕蒙蒙 , 陈宇 , 林思祖 . 6−BA对铝胁迫杉木幼苗抗氧化酶活性的研究 . | 西南林业大学学报 , 2021 , 41 (5) , 172-177 . |
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以杉木实生苗为试验材料,采用单因素试验的方法,选择NAA、IAA、6-BA 3种生长调节剂,各设置3个浓度,研究铝胁迫下不同生长调节剂对杉木幼苗生长及光合特性的影响.结果表明:铝胁迫使杉木幼苗的根长与根冠比受到了抑制,杉木叶片的光合速率(Pn)、气孔导度(Cs)、蒸腾速率(Tr)以及光合色素(叶绿素a、叶绿素b、叶绿素a+b和类胡萝卜素)含量明显降低,胞间二氧化碳浓度(Ci)明显提高.表明NAA、IAA和6-BA等3种生长调节剂促进了根的生长,提高了杉木幼苗的根冠比、Pn、Cs、Tr以及光合色素含量,降低了Ci,有效地减轻铝毒对杉木幼苗造成的不良影响.
Keyword :
光合特性 光合特性 杉木 杉木 生长 生长 生长调节剂 生长调节剂 铝胁迫 铝胁迫
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| GB/T 7714 | 吕蒙蒙 , 武忆寒 , 马志慧 et al. 铝胁迫下生长调节剂对杉木幼苗生长及光合特性的影响 [J]. | 福建农林大学学报(自然科学版) , 2021 , 50 (01) : 69-78 . |
| MLA | 吕蒙蒙 et al. "铝胁迫下生长调节剂对杉木幼苗生长及光合特性的影响" . | 福建农林大学学报(自然科学版) 50 . 01 (2021) : 69-78 . |
| APA | 吕蒙蒙 , 武忆寒 , 马志慧 , 陈宇 , 林思祖 . 铝胁迫下生长调节剂对杉木幼苗生长及光合特性的影响 . | 福建农林大学学报(自然科学版) , 2021 , 50 (01) , 69-78 . |
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Abstract :
以杉木实生苗为材料,采用单因素试验方法,探究不同浓度6-BA对铝胁迫下杉木幼苗根、茎和叶中抗氧化酶活性的影响情况。结果表明:与CK相比,随着6-BA浓度的增加,杉木幼苗根、茎、叶中的丙二醛含量及抗氧化酶活性呈现出不同的变化规律。其中叶片中的丙二醛含量和过氧化物酶活性最高,其次是茎和根;过氧化氢酶活性在根中最强,其次是茎和叶;多酚氧化酶活性在叶片中最弱,其次是根和茎。因此,6-BA降低了不同组织中丙二醛的含量,提高了过氧化氢酶,多酚氧化酶和过氧化物酶的活性,表明6-BA可以在一定程度上减轻铝对杉木的毒害。
Keyword :
6-BA 6-BA 不同组织 不同组织 抗氧化酶活性 抗氧化酶活性 杉木 杉木 铝胁迫 铝胁迫
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| GB/T 7714 | 吕蒙蒙 , 陈宇 , 林思祖 . 6-BA对铝胁迫杉木幼苗抗氧化酶活性的研究 [J]. | 西南林业大学学报(自然科学) , 2021 , 41 (05) : 172-177 . |
| MLA | 吕蒙蒙 et al. "6-BA对铝胁迫杉木幼苗抗氧化酶活性的研究" . | 西南林业大学学报(自然科学) 41 . 05 (2021) : 172-177 . |
| APA | 吕蒙蒙 , 陈宇 , 林思祖 . 6-BA对铝胁迫杉木幼苗抗氧化酶活性的研究 . | 西南林业大学学报(自然科学) , 2021 , 41 (05) , 172-177 . |
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