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学者姓名：黄斌
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Abstract ：
Genebank conservation of pollen is valuable because it makes genetic resources immediately available for use in breeding programs. In the case of Citrus species, conserved anthers or pollen can be easily transported and used to develop new varieties with pathogen resistance and desirable quality and yield traits. The aim of this study was to develop and improve air-desiccation cryopreservation protocols for Citrus cavaleriei and Citrus maxima anthers in genebanks. In the current study, warming, rehydration, and in vitro germination conditions were optimized to achieve high levels of in vitro germination in Citrus pollen for ten cultivars after liquid nitrogen (LN) exposure. The optimal warming, rehydration, and in vitro germination medium formulations affected the germination levels after pollen cryopreservation, with species- and cultivar-dependent effects. The Citrus anthers were dehydrated to the moisture content of 5-14% before LN exposure and warmed at 25 (cryopreserved Citrus anthers with a moisture content of lower than 10%) or 37A degrees C (a moisture content of 10% or higher), then rehydrated, and cultured on medium with 150-g L-1 sucrose, 0.1-g L-1 boric acid, 1.0-g L-1 calcium nitrate, 0.1-g L-1 potassium nitrate, 0.3-g L-1 magnesium sulfate, and 10-g L-1 agar. After 2 yr of storage, in vitro germination levels of Citrus pollen after cryopreservation were significantly higher (> 22% for all ten cultivars) than those of samples that were stored at 4A degrees C (0%). In vitro germination levels of pollen from six of ten cultivars after cryopreservation remained relatively high after 2 yr of storage (38-93%). The highest viability of 93% was obtained for C. cavaleriei '2-3'. The methods identified in the current study could be used to cryopreserve C. cavaleriei and C. maxima anthers.
Keyword ：
Air-desiccation Air-desiccation Citrus Citrus Cryopreservation Cryopreservation In vitro germination In vitro germination Pollen Pollen
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| GB/T 7714 | Zhang, Jin-Mei , Lu, Xin-Xiong , Xin, Xia et al. Cryopreservation of Citrus anthers in the National Crop Genebank of China [J]. | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT , 2017 , 53 (4) : 318-327 . |
| MLA | Zhang, Jin-Mei et al. "Cryopreservation of Citrus anthers in the National Crop Genebank of China" . | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 53 . 4 (2017) : 318-327 . |
| APA | Zhang, Jin-Mei , Lu, Xin-Xiong , Xin, Xia , Yin, Guang-Kun , He, Juan-Juan , Huang, Bin et al. Cryopreservation of Citrus anthers in the National Crop Genebank of China . | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT , 2017 , 53 (4) , 318-327 . |
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