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学者姓名：林同香
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Abstract ：
Background PaBing-II Formula (PB-II) is a traditional Chinese medicine for treating Parkinson's disease (PD). However, owing to the complexity of PB-II and the difficulty in obtaining human dopaminergic neurons (DAn), the mechanism of action of PB-II in PD treatment remains unclear. The aim of this study was to investigate the mechanisms underlying the therapeutic benefits of PB-II in patients with PD.Methods hiPSCs derived DAn were treated with H2O2 to construct the DAn oxidative damage model. SwissTargetPrediction was employed to predict the potential targets of the main compounds in serum after PB-II treatment. Metascape was used to analyze the pathways. Sprague-Dawley rats were used to construct the 6-hydroxydopamine (6-OHDA)-induced PD model, and the duration of administration was four weeks. RNA sequencing was used for Transcriptome analysis to find the signal pathways related to neuronal damage. The associated inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA). We identified PB-II as an Nrf2 activator using antioxidant-responsive element luciferase assay in MDA-MB-231 cells.Results In vitro experiments showed that the treatment of PB-II-treated serum increased the percentage of TH+ cells, decreased inflammation and the apoptosis, reduced cellular reactive oxygen species, and upregulated the expression of Nrf2 and its downstream genes. Pathway analysis of the RNA-seq data of samples before and after the treatment with PB-II-treated serum identified neuron-associated pathways. In vivo experiments demonstrated that PB-II treatment of PD rat model could activate the Nrf2 signaling pathway, protect the midbrain DAn, and improve the symptoms in PD rats.Conclusion PB-II significantly protects DAn from inflammation and oxidative stress via Nrf2 pathway activation. These findings elucidate the roles of PB-II in PD treatment and demonstrate the application of hiPSC-derived DAn in research of Chinese medicine. PaBing-II Formula (PB-II) is a traditional Chinese medicine for treating Parkinson's disease (PD). To investigate the mechanisms underlying the therapeutic benefits of PB-II in patients with PD, we developed human dopaminergic neurons (DAn) oxidative damage models by treating human induced pluripotent stem cells (hiPSCs) derived DAn with H2O2 as well as the 6-hydroxydopamine (6-OHDA)-induced PD rat model. Through in vitro and in vivo studies, we demonstrate that PB-II significantly protects DAn from oxidative stress and inflammation via Nrf2 pathway activation, and is manifested as a treatment for Parkinson's disease.
Keyword ：
DAn DAn hiPSCs hiPSCs inflammation inflammation Nrf2/ARE Nrf2/ARE Parkinson's disease Parkinson's disease ROS ROS
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| GB/T 7714 | Wu, Shouhai , Rong, Cuiping , Lin, Ruishan et al. Chinese medicine PaBing-II protects human iPSC-derived dopaminergic neurons from oxidative stress [J]. | FRONTIERS IN IMMUNOLOGY , 2024 , 15 . |
| MLA | Wu, Shouhai et al. "Chinese medicine PaBing-II protects human iPSC-derived dopaminergic neurons from oxidative stress" . | FRONTIERS IN IMMUNOLOGY 15 (2024) . |
| APA | Wu, Shouhai , Rong, Cuiping , Lin, Ruishan , Ji, Kaiyuan , Lin, Tongxiang , Chen, Weimin et al. Chinese medicine PaBing-II protects human iPSC-derived dopaminergic neurons from oxidative stress . | FRONTIERS IN IMMUNOLOGY , 2024 , 15 . |
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Parkinson's disease (PD) is one of the most common neuro-degenerative diseases characterized by alpha-synuclein accumulation and degeneration of dopaminergic neurons. Employing genome-wide sequencing, we identified a polymorphic USP8 allele (USP8D442G) significantly enriched in Chinese PD patients. To test the involvement of this polymorphism in PD pathogenesis, we derived dopaminergic neurons (DAn) from human-induced pluripotent stem cells (hiPSCs) reprogrammed from fibroblasts of PD patients harboring USP8D442G allele and their healthy siblings. In addition, we knock-in D442G polymorphic site into the endogenous USP8 gene of human embryonic stem cells (hESCs) and derived DAn from these knock-in hESCs to explore their cellular phenotypes and molecular mechanism. We found that expression of USP8D442G in DAn induces the accumulation and abnormal subcellular localization of alpha-Synuclein (alpha-Syn). Mechanistically, we demonstrate that D442G polymorphism enhances the interaction between alpha-Syn and USP8 and thus increases the K63-specific deubiquitination and stability of alpha-Syn . We discover a pathogenic polymorphism for PD that represent a promising therapeutic and diagnostic target for PD.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 Disease prediction Disease prediction Protein structure Protein structure Rs3743044 Rs3743044
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| GB/T 7714 | Wu, Shouhai , Lin, Tongxiang , Xu, Yang . Polymorphic USP8 allele promotes Parkinson's disease by inducing the accumulation of α-synuclein through deubiquitination [J]. | CELLULAR AND MOLECULAR LIFE SCIENCES , 2023 , 80 (12) . |
| MLA | Wu, Shouhai et al. "Polymorphic USP8 allele promotes Parkinson's disease by inducing the accumulation of α-synuclein through deubiquitination" . | CELLULAR AND MOLECULAR LIFE SCIENCES 80 . 12 (2023) . |
| APA | Wu, Shouhai , Lin, Tongxiang , Xu, Yang . Polymorphic USP8 allele promotes Parkinson's disease by inducing the accumulation of α-synuclein through deubiquitination . | CELLULAR AND MOLECULAR LIFE SCIENCES , 2023 , 80 (12) . |
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本发明涉及一种药食同源的中药组合物，具体涉及一种用于治疗/预防糖尿病的中药组合物及其应用，用于治疗/预防糖尿病的中药组合物由以下中药组成：土茯苓、黄芪、当归与甘草；本发明提供的中药组合物对糖尿病具有较好的辅助预防及治疗效果。
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| GB/T 7714 | 林同香 , 林益 , 林相懿 . 一种用于治疗/预防糖尿病的中药组合物及其应用 : CN201911412175.2[P]. | 2019-12-31 . |
| MLA | 林同香 et al. "一种用于治疗/预防糖尿病的中药组合物及其应用" : CN201911412175.2. | 2019-12-31 . |
| APA | 林同香 , 林益 , 林相懿 . 一种用于治疗/预防糖尿病的中药组合物及其应用 : CN201911412175.2. | 2019-12-31 . |
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本发明涉及一种药食同源的中药组合物，具体涉及一种用于治疗/预防II型糖尿病的中药组合物及其应用，用于治疗/预防II型糖尿病的中药组合物由以下中药组成：土茯苓、黄芪与当归；本发明提供的中药组合物对于糖尿病降糖效果明显，适合于早期糖尿病人和或者用于一些糖尿病早期发生的预防性应用的通用中药组合物，也可用于系统治疗的辅助应用。
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| GB/T 7714 | 林同香 , 林益 , 林相懿 . 一种用于治疗/预防II型糖尿病的中药组合物及其应用 : CN201911054004.7[P]. | 2019-10-31 . |
| MLA | 林同香 et al. "一种用于治疗/预防II型糖尿病的中药组合物及其应用" : CN201911054004.7. | 2019-10-31 . |
| APA | 林同香 , 林益 , 林相懿 . 一种用于治疗/预防II型糖尿病的中药组合物及其应用 : CN201911054004.7. | 2019-10-31 . |
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Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra (SN)-striatum circuit, which is associated with glial activation and consequent chronic neuroinflammation. Optimized Yinxieling Formula (OYF) is a Chinese medicine that exerts therapeutical effect and antiinflammation property on psoriasis. Our previous study has proven that pretreatment with OYF could regulate glia-mediated inflammation in an acute mouse model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Given that PD is a chronic degeneration disorder, this study applied another PD animal model induced by striatal injection of 6-hydroxydopamine (6-OHDA) to mimic the progressive damage of the SN-striatum dopamine system in rats. The OYF was administrated in the manner of pretreatment plus treatment. The effects of the OYF on motor behaviors were assessed with the apomorphine-induced rotation test and adjusting steps test. To confirm the effect of OYF on dopaminergic neurons and glia activation in this model, we analyzed the expression of tyrosine hydroxylase (TH) and glia markers, ionized calcium-binding adapter molecule 1 (Iba-1), and glial fibrillary acidic protein (GFAP) in the SN region of the rat PD model. Inflammation-associated factors, including tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), were further evaluated in this model and in interferon-gamma- (INF-gamma-) induced murine macrophages RAW264.7 cells. The results from the in vivo study showed that OYF reversed the motor behavioral dysfunction in 6-OHDA-induced PD rats, upregulated the TH expression, decreased the immunoreactivity of Iba-1 and GFAP, and downregulated the mRNA levels of TNF-alpha and COX-2. The OYF also trended to decrease the mRNA levels of IL-1 beta and iNOS in vivo. The results from the in vitro study showed that OYF significantly decreased the mRNA levels of TNF-alpha, IL-1 beta, IL-6, iNOS, and COX-2. Therefore, this study suggests that OYF exerts antiinflammatory effects, which might be related to the protection of dopaminergic neurons in 6-OHDA-induced chronic neurotoxicity.
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| GB/T 7714 | Wei, Renrong , Rong, Cuiping , Xie, Qingfeng et al. Neuroprotective Effect of Optimized Yinxieling Formula in 6-OHDA-Induced Chronic Model of Parkinson's Disease through the Inflammation Pathway [J]. | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE , 2019 , 2019 . |
| MLA | Wei, Renrong et al. "Neuroprotective Effect of Optimized Yinxieling Formula in 6-OHDA-Induced Chronic Model of Parkinson's Disease through the Inflammation Pathway" . | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2019 (2019) . |
| APA | Wei, Renrong , Rong, Cuiping , Xie, Qingfeng , Wu, Shouhai , Feng, Yuchao , Wang, Ruihua et al. Neuroprotective Effect of Optimized Yinxieling Formula in 6-OHDA-Induced Chronic Model of Parkinson's Disease through the Inflammation Pathway . | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE , 2019 , 2019 . |
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Parkinson's disease (PD) is marked by the progressive degeneration of dopamiergic neurons (DAN) accompanied by glial activation. Thus, inhibiting glial activation that occurs during this disease could be an effective method for treating PD. Optimized Yinxieling Formula (OYF), a Chinese medicinal formula, which is used to efficiently treat autoimmune disease psoriasis, has been proved to display potential immunomodulatory effects in inflammation-associated diseases. This study assessed the therapeutic benefits of OYF on glial-mediated neurointlammation and neuroprotection in PD models in vitro and in vivo. First, the results showed that OYF significantly suppresses 1,1'S-induced proinflammatory cytokine secretion and attenuates the overall inflammatory responses in BV-2 cells. Second, in vivo studies confirm that while the validity of our MPTP-induced PD mouse models possesses activated glia and significant neurobehavioral dysfunction, pretreatment with OYF prevents glial activation and ameliorates movement dysfunction in the MPTP-induced PD mouse models as evaluated by the pole and rotarod tests. Third, transcriptomic analyses were carried out to reveal the underlying molecular mechanism of the OYF treatment. Sixteen pathways were significantly upregulated in the OYF-treated PD model mice, including the cytokine-cytokine receptor interaction, cell adhesion molecules, coagulation, and complement cascades. Fifteen pathways were significantly downregulated in the OYF-treated PD model mice, such as the natural killer cell mediated cytotoxicity, hematopoietic cell lineage, phagosome, and others. These pathways share direct or indirect features of immunomodulation, suggesting that the physiological effects of OYF involve key roles of immune and inflammation regulations. Therefore, we prove that YEF is a useful immunomodulatory formula in developing prevention and treatment methods for neurodegenerative disease PD.
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| GB/T 7714 | Wei, RenRong , Jing OuYang , Lin, WeiXian et al. Curative Anti-Inflammatory Properties of Chinese Optimized Yinxieling Formula in Models of Parkinson's Disease [J]. | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE , 2018 , 2018 . |
| MLA | Wei, RenRong et al. "Curative Anti-Inflammatory Properties of Chinese Optimized Yinxieling Formula in Models of Parkinson's Disease" . | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2018 (2018) . |
| APA | Wei, RenRong , Jing OuYang , Lin, WeiXian , Lin, TongXiang . Curative Anti-Inflammatory Properties of Chinese Optimized Yinxieling Formula in Models of Parkinson's Disease . | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE , 2018 , 2018 . |
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The role of p53 as "a guardian of the genome" has been well established in somatic cells. However, its role in pluripotent stem cells remains much more elusive. Here, we discuss research progress in understanding the role of p53 in pluripotent stem cells and in pluripotent stem cell-like cancer stem cells. The p53 protein, which plays a key role in embryonic stem cells, was first discovered in 2005. Landmark studies of p53-related reprogramming elucidated this protein's importance in induced pluripotent stem cells in 2009. The p53-related safety concerns in pluripotent stem cells have been raised in stem cell-based therapy although the use of iPSCs in therapeutic application is promising. Because cancer stem cells have profiles similar to those of pluripotent stem cells, we also describe potential strategies for studies in cancer stem cells and cancer treatments. The new discoveries of p53 family proteins in pluripotent stem cells have made possible stable progress in stem cell transplantation efficiency and safety, as well as treatment strategies targeting cancer stem cells based on pluripotent stem cell technology.
Keyword ：
Cancer stem cells Cancer stem cells Embryonic stem cells (ESCs) Embryonic stem cells (ESCs) Induced pluripotent stem cells (iPSCs) Induced pluripotent stem cells (iPSCs) P53 P53
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| GB/T 7714 | Lin, Tongxiang , Lin, Yi . p53 switches off pluripotency on differentiation [J]. | STEM CELL RESEARCH & THERAPY , 2017 , 8 . |
| MLA | Lin, Tongxiang et al. "p53 switches off pluripotency on differentiation" . | STEM CELL RESEARCH & THERAPY 8 (2017) . |
| APA | Lin, Tongxiang , Lin, Yi . p53 switches off pluripotency on differentiation . | STEM CELL RESEARCH & THERAPY , 2017 , 8 . |
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Parkinson's disease (PD) is the second most frequent neurodegenerative disease after Alzheimer's disease, which is characterized by a low level of dopamine being expressing in the striatum and a deterioration of dopaminergic neurons (DAn) in the substantia nigra pars compacta. Generation of PD-derived DAn, including differentiation of human embryonic stem cells, human neural stem cells, human-induced pluripotent stem cells, and direct reprogramming, provides an ideal tool to model PD, creating the possibility of mimicking key essential pathological processes and charactering single-cell changes in vitro. Furthermore, thanks to the understanding of molecular neuropathogenesis of PD and new advances in stem-cell technology, it is anticipated that optimal functionally transplanted DAn with targeted correction and transgene-free insertion will be generated for use in cell transplantation. This review elucidates stem-cell technology for modeling PD and offering desired safe cell resources for cell transplantation therapy.
Keyword ：
cell therapy stem cells cell therapy stem cells modeling disease modeling disease Parkinson's disease Parkinson's disease
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| GB/T 7714 | Zhang, Qingxi , Chen, Wanling , Tan, Sheng et al. Stem Cells for Modeling and Therapy of Parkinson's Disease [J]. | HUMAN GENE THERAPY , 2017 , 28 (1) : 85-98 . |
| MLA | Zhang, Qingxi et al. "Stem Cells for Modeling and Therapy of Parkinson's Disease" . | HUMAN GENE THERAPY 28 . 1 (2017) : 85-98 . |
| APA | Zhang, Qingxi , Chen, Wanling , Tan, Sheng , Lin, Tongxiang . Stem Cells for Modeling and Therapy of Parkinson's Disease . | HUMAN GENE THERAPY , 2017 , 28 (1) , 85-98 . |
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The sweet smell of aroma of Jasminum sambac (L.) Ait. is releasing while the flowers are blooming. Although components of volatile oil have been extensively studied, there are problematic issues, such as low efficiency of yield, flavour distortion. Here, the subcritical fluid extraction (SFE) was performed to extract fragrant volatiles from activated carbon that had absorbed the aroma of jasmine flowers. This novel method could effectively obtain main aromatic compounds with quality significantly better than solvent extraction (SE). Based on the analysis data with response surface methodology (RSM), we optimized the extraction conditions which consisted of a temperature of 44 degrees C, a solvent-to-material ratio of 3.5:1, and an extraction time of 53 min. Under these conditions, the extraction yield was 4.91%. Furthermore, the key jasmine essence oil components, benzyl acetate and linalool, increase 7 fold and 2 fold respectively which lead to strong typical smell of the jasmine oil. The new method can reduce spicy components which lead to the essential oils smelling sweeter. Thus, the quality of the jasmine essence oil was dramatically improved and yields based on the key component increased dramatically. Our results provide a new effective technique for extracting fragrant volatiles from jasmine flowers.
Keyword ：
fragrant volatiles fragrant volatiles Jasminum sambac (L.) Ait. Jasminum sambac (L.) Ait. response surface methodology response surface methodology subcritical dimethyl ether extraction subcritical dimethyl ether extraction
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| GB/T 7714 | Ye, Qiuping , Jin, Xinyi , Zhu, Xinliang et al. An Efficient Extraction Method for Fragrant Volatiles from Jasminum sambac (L.) Alt [J]. | JOURNAL OF OLEO SCIENCE , 2015 , 64 (6) : 645-652 . |
| MLA | Ye, Qiuping et al. "An Efficient Extraction Method for Fragrant Volatiles from Jasminum sambac (L.) Alt" . | JOURNAL OF OLEO SCIENCE 64 . 6 (2015) : 645-652 . |
| APA | Ye, Qiuping , Jin, Xinyi , Zhu, Xinliang , Lin, Tongxiang , Hao, Zhilong , Yang, Qian . An Efficient Extraction Method for Fragrant Volatiles from Jasminum sambac (L.) Alt . | JOURNAL OF OLEO SCIENCE , 2015 , 64 (6) , 645-652 . |
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The genomes of two rice cultivars, Nipponbare and 93-11, have been well studied. However, there is little available genetic information about nutraceutical rice cultivars. To remedy this situation, the present study aimed to provide a basic genetic landscape of nutraceutical rice. The genome of Black-1, a black pericarp rice containing higher levels of anthocyanins, flavonoids, and a more potent antioxidant capacity, was sequenced at a parts per thousand yen30 x coverage using Solexa sequencing technology. The complete sequences of Black-1 genome shared more consensus sequences with indica cultivar 93-11 than with Nipponbare. With reference to the 93-11 genome, Black-1 contained 675,207 single-nucleotide polymorphisms, 43,130 insertions and deletions (1-5 bp), 1,770 copy number variations, and 10,911 presence/absence variations. These variations were observed to reside preferentially in Myb domains, NB-ARC domains and kinase domains, providing clues to the diversity of biological functions or secondary metabolisms in this cultivar. Intriguingly, 496 unique genes were identified by comparing it with the genomes of these two rice varieties; among the genes, 119 genes participate in the biosynthesis of secondary metabolites. Furthermore, several unique genes were predicted to be involved in the anthocyanins synthesis pathway. The genome-wide landscape of Black-1 uncovered by this study represents a valuable resource for further studies and for breeding nutraceutical rice varieties.
Keyword ：
Black pericarp rice Black pericarp rice Copy number variation Copy number variation INDEL INDEL Nutraceutical Nutraceutical Presence/absence variation Presence/absence variation Single-nucleotide polymorphism Single-nucleotide polymorphism
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| GB/T 7714 | Lin, Juncheng , Cheng, Zuxin , Xu, Ming et al. Genome re-sequencing and bioinformatics analysis of a nutraceutical rice [J]. | MOLECULAR GENETICS AND GENOMICS , 2015 , 290 (3) : 955-967 . |
| MLA | Lin, Juncheng et al. "Genome re-sequencing and bioinformatics analysis of a nutraceutical rice" . | MOLECULAR GENETICS AND GENOMICS 290 . 3 (2015) : 955-967 . |
| APA | Lin, Juncheng , Cheng, Zuxin , Xu, Ming , Huang, Zhiwei , Yang, Zhijian , Huang, Xinying et al. Genome re-sequencing and bioinformatics analysis of a nutraceutical rice . | MOLECULAR GENETICS AND GENOMICS , 2015 , 290 (3) , 955-967 . |
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