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学者姓名：张峰
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Abstract ：
本研究结合酶抑制原理和金纳米双锥体比色策略，构建了多色可视化的比色检测体系，从而实现了果蔬和中药材中有机磷和氨基甲酸酯类农药残留的的现场半定量检测和仪器定量检测。我们分别考察了酶抑制、酶解反应和金纳米双锥体刻蚀过程中的实验条件，确定了果蔬和中药材中有机磷和氨基甲酸酯类农药残留的最佳检测条件：抑制时间15 min，底物氯化乙酰硫代胆碱浓度1.5 mmol/L，十六烷基三甲基溴化铵浓度40 mmol/L、氯金酸浓度0.4 mmol/L。对果蔬和中药材中敌敌畏、克百威、灭多威、丙溴磷和敌百虫的加标回收实验结果显示，回收率在74.9～93.7%，相对标准偏差小于4%。样品同时使用该方法和传统的酶抑制率法测定，两者的测定结果有较好的吻合度。
Keyword ：
中药材 中药材 农药残留 农药残留 果蔬 果蔬 酶抑制率 酶抑制率 金纳米双锥体 金纳米双锥体
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| GB/T 7714 | 曾晓雪 , 张峰 , 王斌 . 果蔬和中药材中农药残留的可视化检测研究 [J]. | 福建分析测试 , 2025 , 34 (01) : 1-7 . |
| MLA | 曾晓雪 等. "果蔬和中药材中农药残留的可视化检测研究" . | 福建分析测试 34 . 01 (2025) : 1-7 . |
| APA | 曾晓雪 , 张峰 , 王斌 . 果蔬和中药材中农药残留的可视化检测研究 . | 福建分析测试 , 2025 , 34 (01) , 1-7 . |
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The cell wall is an essential dynamic structure for shielding fungus from environmental stress, and its synthesizing and remodeling are regulated by the cell wall integrity (CWI) pathway. Here, we explored the roles of a putative downstream effector AflRlm1 of CWI pathway in Aspergillus flavus. The results showed that AflRlm1 played a positive role in conidia production, sclerotium formation, aflatoxin biosynthesis, and pathogenicity. Furthermore, we provided evidence for the physical connection between AflRlm1 and AflSlt2 and determined the role of AflSlt2 in the phosphorylation of AflRlm1. Then, we discovered the importance of WSCs (cell wall integrity and stress response component) to the CWI signal and the process of AflRlm1 transferring to the nucleus after receiving the signal. Overall, this study clarified the transmission process of CWI signals and proves that the CWI pathway plays a key role in the development of A. flavus and the production of aflatoxin combined with transcriptome data analysis.
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| GB/T 7714 | Tan, Can , Deng, Ji-Li , Zhang, Feng et al. CWI pathway participated in vegetative growth and pathogenicity through a downstream effector AflRlm1 in Aspergillus flavus [J]. | ISCIENCE , 2021 , 24 (10) . |
| MLA | Tan, Can et al. "CWI pathway participated in vegetative growth and pathogenicity through a downstream effector AflRlm1 in Aspergillus flavus" . | ISCIENCE 24 . 10 (2021) . |
| APA | Tan, Can , Deng, Ji-Li , Zhang, Feng , Zhu, Zhuo , Yan, Li-Juan , Zhang, Meng-Juan et al. CWI pathway participated in vegetative growth and pathogenicity through a downstream effector AflRlm1 in Aspergillus flavus . | ISCIENCE , 2021 , 24 (10) . |
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Aflatoxin contamination in food and feed products has been brought into sharp focus over the last few decades in the world. However, there is no effective strategy for solving the problem thus far. Therefore, basic research on the aflatoxin-producer Aspergillus flavus is an urgent need. The vital role of mitogen-activated protein kinases (MAPKs) in signal transduction has been documented in various pathogenic fungi, but their functions in A. flavus have rarely been investigated. Herein, we characterized the detailed function of one of these MAPKs, AflSlt2. Targeted deletion of AflSlt2 gene indicates that this kinase is required for vegetative growth, conidia generation, and sclerotium formation. The analysis of AflSlt2 deletion mutant revealed hypersensitivity to cell wall-damaging chemicals and resistance against hydrogen peroxide. Interestingly, the ability of the Delta AflSlt2 mutant to generate aflatoxins in medium was significantly increased compared to wild type. However, a pathogenicity assay indicated that the Delta AflSlt2 mutant was deficient in peanut infection. Site-directed mutation study uncovered that the function of AflSlt2 was dependent on the phosphorylated residues (Thr-186 and Tyr-188) within the activation loop and the phosphotransfer residue (Lys-52) within the subdomain II. Interestingly, an autophosphorylation mutant of AflSlt2 (AflSlt2(R66S)) displayed wild type-like phenotypes. Bringing these observations together, we propose that Slt2-MAPK pathway is involved in development, stress response, aflatoxin biosynthesis, and pathogenicity in A. flavus. This study may be useful to unveil the regulation mechanism of aflatoxin biosynthesis and provide strategy to control A. flavus contamination.
Keyword ：
Aflatoxin Aflatoxin AflSlt2 AflSlt2 Aspergillus flavus Aspergillus flavus Mitogen-activated protein kinases Mitogen-activated protein kinases
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| GB/T 7714 | Zhang, Feng , Geng, Longpo , Deng, Jili et al. The MAP kinase AflSlt2 modulates aflatoxin biosynthesis and peanut infection in the fungus Aspergillus flavus [J]. | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY , 2020 , 322 . |
| MLA | Zhang, Feng et al. "The MAP kinase AflSlt2 modulates aflatoxin biosynthesis and peanut infection in the fungus Aspergillus flavus" . | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 322 (2020) . |
| APA | Zhang, Feng , Geng, Longpo , Deng, Jili , Huang, Luhua , Zhong, Hong , Xin, Sijie et al. The MAP kinase AflSlt2 modulates aflatoxin biosynthesis and peanut infection in the fungus Aspergillus flavus . | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY , 2020 , 322 . |
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The filament fungal pathogen, Aspergillus flavus, spreads worldwide and contaminates several important crops. Histone posttranslational modifications are deeply involved in fungal development and virulence, but the biological function of the histone methyltransferase AflSet1 in A. flavus is still unknown. In the study, Aflset1 deletion strain was constructed through homologous recombination, and it was found that AflSet1 up-regulates hyphae growth, and promotes conidiation by sporulation regulation genes: abaA and brlA. It was also found that AflSet1 involves in sclerotia formation and AFB1 biosynthesis via sclerotia related transcriptional factors and orthodox AFB1 synthesis pathway, respectively. Crop models revealed that AflSet1 plays critical roles in colonization and AFB1 production on crop kernels. Lipase activity analysis suggested that AflSet1 affects fungal virulence to crops via digestive enzymes. Stresses tests revealed that AflSet1 is deeply involved in fungal resistance against osmotic, oxidative and cell membrane stress. The preparation of N_SET, SET domain deletion mutants and H988K mutant revealed that both domains play critical roles in fungal development and AFB1 production, and that H988 is very important in executing biological functions on morphogenesis and AFB1 synthesis. Subcellular location analysis revealed that AflSet1 is stably accumulated in nuclei in both spore germination and hyphae growth stages, even under the stress of SDS. Through immunoblot analysis, it was found that AflSet1 methylates H3K4me2 and me3 as well as H3K9me2. This study provides a solid evidence to discover the biological functions of histone methyltransferase in pathogenic fungi.
Keyword ：
AflSet1 AflSet1 Aspergillus flavus Aspergillus flavus histone methyltransferase histone methyltransferase morphology morphology virulence virulence
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| GB/T 7714 | Liu, Yaju , Zhang, Mengjuan , Xie, Rui et al. The Methyltransferase AflSet1 Is Involved in Fungal Morphogenesis, AFB1 Biosynthesis, and Virulence of Aspergillus flavus [J]. | FRONTIERS IN MICROBIOLOGY , 2020 , 11 . |
| MLA | Liu, Yaju et al. "The Methyltransferase AflSet1 Is Involved in Fungal Morphogenesis, AFB1 Biosynthesis, and Virulence of Aspergillus flavus" . | FRONTIERS IN MICROBIOLOGY 11 (2020) . |
| APA | Liu, Yaju , Zhang, Mengjuan , Xie, Rui , Zhang, Feng , Wang, Sen , Pan, Xiaohua et al. The Methyltransferase AflSet1 Is Involved in Fungal Morphogenesis, AFB1 Biosynthesis, and Virulence of Aspergillus flavus . | FRONTIERS IN MICROBIOLOGY , 2020 , 11 . |
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Aspergillus flavus represents an important fungal pathogen, causing severe economic losses in crops. The mitogen-activated protein (MAP) kinase signaling pathway contributes to many physiological processes, but its precise role in A. flavus is not yet fully understood. In this study, we focused on the AflBck1 gene, which encodes a MAP kinase kinase kinase of the Slt2-MAPK pathway. Targeted deletion of AflBck1 led to a significant defect in growth and development, and a AflBck1-deleted mutant (Delta AflBck1) showed higher sensitivity to cell-wall stress than wild type (WT). Importantly, we observed that Delta AflBck1 displayed an enhanced ability to produce aflatoxin, a potential carcinogenic mycotoxin. However, the pathogenicity of the Delta AflBck1 mutant was markedly reduced in peanut seeds. We also presented evidence that AflBck1 was genetically epistatic to AflMkk2 in the Slt2-MAPK pathway. Finally, we found that loss of the proline-rich region at the N terminus of AflBck1 affected the reproduction of A. flavus. Collectively, this study not only extended the understanding that the MAPK pathway regulated A. flavus pathogenicity but also provided a possible strategy to control A. flavus contamination.
Keyword ：
aflatoxin aflatoxin Aspergillus flavus Aspergillus flavus mitogen-activated protein kinase pathway mitogen-activated protein kinase pathway pathogenicity pathogenicity
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| GB/T 7714 | Zhang, Feng , Huang, Luhua , Deng, Jili et al. A Cell Wall Integrity-Related MAP Kinase Kinase Kinase AflBck1 Is Required for Growth and Virulence in Fungus Aspergillus flavus [J]. | MOLECULAR PLANT-MICROBE INTERACTIONS , 2020 , 33 (4) : 680-692 . |
| MLA | Zhang, Feng et al. "A Cell Wall Integrity-Related MAP Kinase Kinase Kinase AflBck1 Is Required for Growth and Virulence in Fungus Aspergillus flavus" . | MOLECULAR PLANT-MICROBE INTERACTIONS 33 . 4 (2020) : 680-692 . |
| APA | Zhang, Feng , Huang, Luhua , Deng, Jili , Tan, Can , Geng, Longpo , Liao, Yun et al. A Cell Wall Integrity-Related MAP Kinase Kinase Kinase AflBck1 Is Required for Growth and Virulence in Fungus Aspergillus flavus . | MOLECULAR PLANT-MICROBE INTERACTIONS , 2020 , 33 (4) , 680-692 . |
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本发明提供了一株不产黄曲霉毒素菌株ΔAflsnt2及防治黄曲霉污染的方法，所述的不产毒株中黄曲霉致病相关基因afllsnt2基本上不表达。本发明有利于在黄曲霉毒素污染的早期从根本上解除黄曲霉毒素的产生，积累，进而有效控制和降低作物受到的产毒黄曲霉感染率，达到有效控制黄曲霉毒素的污染目的。与上述的黄曲霉毒素污染检测手段和污染后解毒技术相比，本发明从源头上控制黄曲霉的污染，成本显著降低。
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| GB/T 7714 | 庄振宏 , 刘亚举 , 胡育乐 et al. 一株不产黄曲霉毒素菌株ΔAflsnt2及防治黄曲霉污染的应用 : CN201910107687.1[P]. | 2019-02-02 . |
| MLA | 庄振宏 et al. "一株不产黄曲霉毒素菌株ΔAflsnt2及防治黄曲霉污染的应用" : CN201910107687.1. | 2019-02-02 . |
| APA | 庄振宏 , 刘亚举 , 胡育乐 , 汪世华 , 郭志强 , 张孟娟 et al. 一株不产黄曲霉毒素菌株ΔAflsnt2及防治黄曲霉污染的应用 : CN201910107687.1. | 2019-02-02 . |
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Aspergillus flavus is an opportunistic pathogenic fungus for both plant and animal that produces carcinogenic toxins termed aflatoxins (AFs). To identify possible genetic targets to reduce AF contamination, in this study, we have characterized a novel A. flavus Set3, and it shares sequence homology with the yeast protein Set3. The set3 deletion mutants present no difference in growth rate but alterations in asexual development and secondary metabolite production when compared to the A. flavus wild type. Specifically, deletion of set3 gene decreases conidiophore formation and conidial production through downregulating expression of brlA and abaA genes. In addition, normal levels of set3 are required for sclerotial development and expression of sclerotia-related genes nsdC and scIR. Further analyses demonstrated that Set3 negatively regulates AF production as well as the concomitant expression of genes in the AF gene cluster. Importantly, our results also display that A. flavus Set3 is involved in crop kernel colonization. Taking together, these results reveal that a novel Set3 plays crucial roles in morphological development, secondary metabolism, and fungal virulence in A. flavus.
Keyword ：
aflatoxin biosynthesis aflatoxin biosynthesis Aspergillus flavus Aspergillus flavus regulate regulate reproduction reproduction Set3 Set3
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| GB/T 7714 | Lan, Huahui , Wu, Lianghuan , Fan, Kun et al. Set3 Is Required for Asexual Development, Aflatoxin Biosynthesis, and Fungal Virulence in Aspergillus flavus [J]. | FRONTIERS IN MICROBIOLOGY , 2019 , 10 . |
| MLA | Lan, Huahui et al. "Set3 Is Required for Asexual Development, Aflatoxin Biosynthesis, and Fungal Virulence in Aspergillus flavus" . | FRONTIERS IN MICROBIOLOGY 10 (2019) . |
| APA | Lan, Huahui , Wu, Lianghuan , Fan, Kun , Sun, Ruilin , Yang, Guang , Zhang, Feng et al. Set3 Is Required for Asexual Development, Aflatoxin Biosynthesis, and Fungal Virulence in Aspergillus flavus . | FRONTIERS IN MICROBIOLOGY , 2019 , 10 . |
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Histone deacetylases (HDACs) always function as corepressors and sometimes as coactivators in the regulation of fungal development and secondary metabolite production. However, the mechanism through which HDACs play positive roles in secondary metabolite production is still unknown. Here, classical HDAC enzymes were identified and analyzed in Aspergillus flavus, a fungus that produces one of the most carcinogenic secondary metabolites, aflatoxin B-1 (AFB1). Characterization of the HDACs revealed that a class I family HDAC, HosA, played crucial roles in growth, reproduction, the oxidative stress response, AFB1 biosynthesis, and pathogenicity. To a lesser extent, a class II family HDAC, HdaA, was also involved in sclerotia formation and AFB1 biosynthesis. An in vitro analysis of HosA revealed that its HDAC activity was considerably diminished at nanomolar concentrations of trichostatin A. Notably, chromatin immunoprecipitation experiments indicated that HosA bound directly to AFB1 biosynthesis cluster genes to regulate their expression. Finally, we found that a transcriptional regulator, SinA, interacts with HosA to regulate fungal development and AFB1 biosynthesis. Overall, our results reveal a novel mechanism by which classical HDACs mediate the induction of secondary metabolite genes in fungi.
Keyword ：
fungal development fungal development fungal effectors fungal effectors
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| GB/T 7714 | Lan, Huahui , Wu, Lianghuan , Sun, Ruilin et al. The HosA Histone Deacetylase Regulates Aflatoxin Biosynthesis Through Direct Regulation of Aflatoxin Cluster Genes [J]. | MOLECULAR PLANT-MICROBE INTERACTIONS , 2019 , 32 (9) : 1210-1228 . |
| MLA | Lan, Huahui et al. "The HosA Histone Deacetylase Regulates Aflatoxin Biosynthesis Through Direct Regulation of Aflatoxin Cluster Genes" . | MOLECULAR PLANT-MICROBE INTERACTIONS 32 . 9 (2019) : 1210-1228 . |
| APA | Lan, Huahui , Wu, Lianghuan , Sun, Ruilin , Keller, Nancy P. , Yang, Kunlong , Ye, Liuqing et al. The HosA Histone Deacetylase Regulates Aflatoxin Biosynthesis Through Direct Regulation of Aflatoxin Cluster Genes . | MOLECULAR PLANT-MICROBE INTERACTIONS , 2019 , 32 (9) , 1210-1228 . |
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蛋白质工程是一个新兴领域,由于学科涉及面广,前沿知识和先进技术较多,学生在学习上存在一定难度。为了提升教学质量,激发学生自主创新潜力,十几年来,福建农林大学课程组不断对该课程进行教学改革的探索,目前已初见成效。该文分别从课程设臵、教材建设、教学方式等方面探析了具体的改革举措,供同行参考。
Keyword ：
实践课 实践课 教学改革 教学改革 理论课 理论课 蛋白质工程 蛋白质工程
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| GB/T 7714 | 袁军 , 张峰 , 贾坤志 et al. 蛋白质工程课程教学改革的实践与启示 [J]. | 海峡科学 , 2019 , (03) : 80-82 . |
| MLA | 袁军 et al. "蛋白质工程课程教学改革的实践与启示" . | 海峡科学 03 (2019) : 80-82 . |
| APA | 袁军 , 张峰 , 贾坤志 , 王宇 , 汪世华 . 蛋白质工程课程教学改革的实践与启示 . | 海峡科学 , 2019 , (03) , 80-82 . |
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生物科学研究的国际化合作、生物科学知识的国际化共享以及生物科学人才的国际化训练是生物领域的发展趋势和发展方向。这种国际化也是生物教学改革的必然顺应的潮流。在很多高校的本科生物专业中,分子生物学是一门核心课程。为了实现国际化分子生物学创新人才培养目标,我们开展了分子生物学教学改革。该文从优化课程计划、精选课程教材、创新教学方法、提升师资力量四方面进行了详细的阐述,总结了近几年分子生物学课程教学改革所取得的成效和经验。同时,也对教学改革中所遇到的问题进行了探讨。
Keyword ：
分子生物学 分子生物学 创新人才 创新人才 教学改革 教学改革
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| GB/T 7714 | 袁军 , 吴瑞美 , 张峰 et al. 生命科学国际化创新人才培养目标驱动下的分子生物学课程教学改革与实践 [J]. | 海峡科学 , 2019 , (02) : 90-93 . |
| MLA | 袁军 et al. "生命科学国际化创新人才培养目标驱动下的分子生物学课程教学改革与实践" . | 海峡科学 02 (2019) : 90-93 . |
| APA | 袁军 , 吴瑞美 , 张峰 , 汪世华 . 生命科学国际化创新人才培养目标驱动下的分子生物学课程教学改革与实践 . | 海峡科学 , 2019 , (02) , 90-93 . |
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