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学者姓名：邹华松
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青枯雷尔氏菌(Ralstonia solanacearum)利用Ⅲ型分泌系统(Type three secretion system, T3SS)将自身合成的100多个效应蛋白注入寄主植物细胞引起免疫反应的变化。本文报道青枯菌GMI1000效应蛋白RipAF1对寄主植物先天免疫反应的诱导作用。在本氏烟草植物上瞬时表达RipAF1,显著地诱导了PTI标志基因NbAcre31、NbPti5和NbGras2的增强表达，伴随着胼胝质的累积。酵母双杂交实验从烟草cDNA文库中筛选到与RipAF1互作的NbBAK1的C端序列，随后的荧光素酶和双分子荧光互补实验表明RipAF1与BAK1在植物体内相互作用。在GMI1000菌株中将ripAF1缺失突变，在营养丰富和基本培养基中生长与野生型没有明显区别，灌根接种番茄后致病力增强，在番茄植物体内的繁殖速度变快。这些结果表明RipAF1是一个诱导寄主植物PTI反应的效应蛋白，并且负向影响青枯菌的致病力。
Keyword ：
PTI PTI RipAF1 RipAF1 Ⅲ型分泌系统 Ⅲ型分泌系统 致病力 致病力 青枯菌 青枯菌
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| GB/T 7714 | 吴薇 , 邹华松 . 青枯菌效应蛋白RipAF1对植物PTI的诱导作用 [J]. | 植物病理学报 , 2023 , 53 (05) : 852-862 . |
| MLA | 吴薇 等. "青枯菌效应蛋白RipAF1对植物PTI的诱导作用" . | 植物病理学报 53 . 05 (2023) : 852-862 . |
| APA | 吴薇 , 邹华松 . 青枯菌效应蛋白RipAF1对植物PTI的诱导作用 . | 植物病理学报 , 2023 , 53 (05) , 852-862 . |
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Ralstonia solanacearum is a devastating bacterial pathogen responsible for bacterial wilt in various crops. It employs the Type III Secretion System (T3SS) to deliver effectors known as R. solanacearum injected proteins (Rips) into host plant cells. These Rips play a critical role in the pathogenicity of the bacterium, yet their mechanisms of action remain incompletely understood. In this study, we screened 13 Rips from R. solanacearum strain FJ1003 and discovered that RipAW and RipAU possess the ability to suppress RNA silencing, an essential gene regulatory mechanism in plants. Moreover, we demonstrated heightened susceptibility to R. solanacearum and potato virus X in transgenic Nicotiana benthamiana overexpressing RipAW. Silencing of Dicer-like 1 (DCL1) or RNA-dependent RNA polymerase 6 (RDR6) promoted the infection of R. solanacearum in N. benthamiana. Arabidopsis with defects in Argonaute 1 (AGO1), DCL1 or RDR6 also showed increased susceptibility to R. solanacearum compared to wildtype Arabidopsis. These findings collectively suggest a scenario in which certain Rips interfere with host plant RNA silencing, and RNA silencing suppression may be one of the mechanisms employed by Rips to promote virulence.
Keyword ：
RNA silencing RNA silencing RNA silencing suppressor RNA silencing suppressor R. solanacearum injected proteins R. solanacearum injected proteins
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| GB/T 7714 | Qiu, Ping , Xu, Yixing , Chen, Lihong et al. Some Type III effectors of Ralstonia solanacearum may suppress host RNA silencing for function [J]. | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY , 2023 , 128 . |
| MLA | Qiu, Ping et al. "Some Type III effectors of Ralstonia solanacearum may suppress host RNA silencing for function" . | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY 128 (2023) . |
| APA | Qiu, Ping , Xu, Yixing , Chen, Lihong , Lin, Wenzhong , Zou, Huasong , Wu, Zujian et al. Some Type III effectors of Ralstonia solanacearum may suppress host RNA silencing for function . | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY , 2023 , 128 . |
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Copper is an essential element for microbes as it is involved in many redox reactions. Numerous resistance systems have been evolved in microbes to maintain copper homeostasis under copper stress conditions. These systems are responsible for the influx and efflux of copper ions in the cells. In phytopathogenic bacteria, copper ions play essential roles during disease development in plants. Copper-based chemicals are extensively used for control of diseases caused by bacteria, which leads to induced pathogen resistance derived from various copper resistance systems. Previous studies have shown that copper ions are harnessed by host plants to protect against bacterial infections, triggering immune responses through activation of defence signalling pathways. Thus, it was anticipated that bacterial copper resistance could play an alternative role in adaptation to plant immunity. This review summarizes current knowledge of copper resistance systems in plant pathogenic bacteria, which may provide a new perspective of molecular mechanisms associated with bacterial adaptation in host plants.
Keyword ：
bacterial copper bacterial copper plant immunity plant immunity resistance resistance Resistance systems Resistance systems signalling pathways signalling pathways stress conditions stress conditions
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| GB/T 7714 | Fan, Xiaojing , Saleem, Tahira , Zou, Huasong . Copper resistance mechanisms in plant pathogenic bacteria [J]. | PHYTOPATHOLOGIA MEDITERRANEA , 2022 , 61 (1) : 129-138 . |
| MLA | Fan, Xiaojing et al. "Copper resistance mechanisms in plant pathogenic bacteria" . | PHYTOPATHOLOGIA MEDITERRANEA 61 . 1 (2022) : 129-138 . |
| APA | Fan, Xiaojing , Saleem, Tahira , Zou, Huasong . Copper resistance mechanisms in plant pathogenic bacteria . | PHYTOPATHOLOGIA MEDITERRANEA , 2022 , 61 (1) , 129-138 . |
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Due to its high genetic diversity and broad host range, Ralstonia solanacearum, the causative phytopathogen of the bacterial wilt (BW) disease, is considered a species complex. The R. solanacearum strain FJ1003 belonged to phylotype I, and was isolated from the Fuzhou City in Fujian Province of China. The pathogen show host specificity and infects tobacco, especially in the tropical and subtropical regions. To elucidate the pathogenic mechanisms of FJ1003 infecting tobacco, a complete genome sequencing of FJ1003 using single-molecule real-time (SMRT) sequencing technology was performed. The full genome size of FJ1003 was 5.90 Mb (GC%, 67%), containing the chromosome (3.7 Mb), megaplasmid (2.0 Mb), and small plasmid (0.2 Mb). A total of 5133 coding genes (3446 and 1687 genes for chromosome and megaplasmid, respectively) were predicted. A comparative genomic analysis with other strains having the same and different hosts showed that the FJ1003 strain had 90 specific genes, possibly related to the host range of R. solanacearum. Horizontal gene transfer (HGT) was widespread in the genome. A type III effector protein (Rs_T3E_Hyp14) was present on both the prophage and genetic island (GI), suggesting that this gene might have been acquired from other bacteria via HGT. The Rs_T3E_Hyp14 was proved to be a virulence factor in the pathogenic process of R. solanacearum through gene knockout strategy, which affects the pathogenicity and colonization ability of R. solanacearum in the host. Therefore, this study will improve our understanding of the virulence of R. solanacearum and provide a theoretical basis for tobacco disease resistance breeding.
Keyword ：
bacterial wilt bacterial wilt disease resistance disease resistance effector proteins effector proteins genome sequencing genome sequencing Ralstonia solanacearum Ralstonia solanacearum Rs_T3E_Hyp14 Rs_T3E_Hyp14 tobacco tobacco
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| GB/T 7714 | Chen, Kun , Zhuang, Yuhui , Wang, Lihui et al. Comprehensive genome sequence analysis of the devastating tobacco bacterial phytopathogen Ralstonia solanacearum strain FJ1003 [J]. | FRONTIERS IN GENETICS , 2022 , 13 . |
| MLA | Chen, Kun et al. "Comprehensive genome sequence analysis of the devastating tobacco bacterial phytopathogen Ralstonia solanacearum strain FJ1003" . | FRONTIERS IN GENETICS 13 (2022) . |
| APA | Chen, Kun , Zhuang, Yuhui , Wang, Lihui , Li, Huaqi , Lei, Taijie , Li, Mengke et al. Comprehensive genome sequence analysis of the devastating tobacco bacterial phytopathogen Ralstonia solanacearum strain FJ1003 . | FRONTIERS IN GENETICS , 2022 , 13 . |
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Simple Summary Tobacco bacterial wilt caused by Ralstonia solanacearum brings large economic losses every year. Currently, an increasing number of biocontrol agents have been widely used in the control of this disease, but they cannot replace chemical agents, mostly due to the poorer control effect. Therefore, in this study, the avirulence gene ripAA from Ralstonia solanacearum, which determines incompatible interactions with tobacco plants, was introduced into the biocontrol agent Pseudomonas mosselii to increase the efficacy against Ralstonia solanacearum. The newly engineered strain can improve the systemic resistance and elicit a primary immune response of plants. Our research not only provides a new strategy for the genetic modification of biocontrol agents, in which a number of avirulence genes from the pathogen or plant can be tested to be expressed in different biocontrol agents to antagonize this plant disease, but also helps the study of the interaction between the phytopathogenic avirulence gene and the host. The environmental bacterium Pseudomonas mosselii produces antagonistic secondary metabolites with inhibitory effects on multiple plant pathogens, including Ralstonia solanacearum, the causal agent of bacterial wilt. In this study, an engineered P. mosselii strain was generated to express R. solanacearum ripAA, which determines the incompatible interactions with tobacco plants. The ripAA gene, together with its native promoter, was integrated into the P. mosselii chromosome. The resulting strain showed no difference in antimicrobial activity against R. solanacearum. Promoter-LacZ fusion and RT-PCR experiments demonstrated that the ripAA gene was transcribed in culture media. Compared with that of the wild type, the engineered strain reduced the disease index by 9.1% for bacterial wilt on tobacco plants. A transcriptome analysis was performed to identify differentially expressed genes in tobacco plants, and the results revealed that ethylene- and jasmonate-dependent defense signaling pathways were induced. These data demonstrates that the engineered P. mosselii expressing ripAA can improve biological control against tobacco bacterial wilt by the activation of host defense responses.
Keyword ：
bacterial wilt bacterial wilt control efficacy control efficacy defense signaling pathway defense signaling pathway Pseudomonas mosselii Pseudomonas mosselii ripAA ripAA
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| GB/T 7714 | Zhuo, Tao , Chen, Shiting , Wang, Dandan et al. Expression of the ripAA Gene in the Soilborne Pseudomonas mosselii Can Promote the Control Efficacy against Tobacco Bacterial Wilt [J]. | BIOLOGY-BASEL , 2022 , 11 (8) . |
| MLA | Zhuo, Tao et al. "Expression of the ripAA Gene in the Soilborne Pseudomonas mosselii Can Promote the Control Efficacy against Tobacco Bacterial Wilt" . | BIOLOGY-BASEL 11 . 8 (2022) . |
| APA | Zhuo, Tao , Chen, Shiting , Wang, Dandan , Fan, Xiaojing , Zhang, Xiaofeng , Zou, Huasong . Expression of the ripAA Gene in the Soilborne Pseudomonas mosselii Can Promote the Control Efficacy against Tobacco Bacterial Wilt . | BIOLOGY-BASEL , 2022 , 11 (8) . |
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The Cylindrocladium black rot caused by Calonectria ilicicola is a destructive disease affecting a broad range of crops. Herein, we study virulence-associated genes of C. ilicicola Ci14017 isolated from diseased peanut roots (Arachis hypogaea L.). Ci14017 was identified via phylogenetic analysis of the internal transcribed spacer region and standard Koch's postulate testing. Virulence-associated genes were based on genome analyses and comparative analysis of transcriptome and proteome profiles of sensitive and resistant peanut cultivars. Ci14017 identified as C. ilicicola has a 66 Mb chromosome with 18,366 predicted protein-coding genes. Overall, 46 virulence-associated genes with enhanced expression levels in the sensitive cultivars were identified. Sequence analysis indicated that the 46 gene products included two merops proteins, eight carbohydrate-active enzymes, seven cytochrome P450 enzymes, eight lipases, and 20 proteins with multi-conserved enzyme domains. The results indicate a complex infection mechanism employed by Ci14017 for causing Cylindrocladium black rot in peanuts.
Keyword ：
Calonectria ilicicola Calonectria ilicicola Cylindrocladium peanut black rot Cylindrocladium peanut black rot genome genome proteome proteome transcriptome transcriptome virulence-associated gene virulence-associated gene
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| GB/T 7714 | Chen, Xinyu , Luo, Mei , Wu, Wei et al. Virulence-Associated Genes of Calonectria ilicola, Responsible for Cylindrocladium Black Rot [J]. | JOURNAL OF FUNGI , 2022 , 8 (8) . |
| MLA | Chen, Xinyu et al. "Virulence-Associated Genes of Calonectria ilicola, Responsible for Cylindrocladium Black Rot" . | JOURNAL OF FUNGI 8 . 8 (2022) . |
| APA | Chen, Xinyu , Luo, Mei , Wu, Wei , Dong, Zhangyong , Zou, Huasong . Virulence-Associated Genes of Calonectria ilicola, Responsible for Cylindrocladium Black Rot . | JOURNAL OF FUNGI , 2022 , 8 (8) . |
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通过体内外生长曲线和表型观察试验，分析不同浓度β-蒎烯对柑橘溃疡病菌(Xcc 29-1)的生长抑制作用和柑橘溃疡病症状形成的影响。利用β-葡萄糖苷酸酶基因的定性检测和实时荧光定量PCR分析不同浓度β-蒎烯对Xcc 29-1Ⅲ型分泌系统相关基因hrp G的诱导作用。结果表明:随着浓度的升高，β-蒎烯对Xcc 29-1的生长抑制增强，0.2μg·mL~(-1)的β-蒎烯能显著抑制溃疡病菌的生长繁殖，培养基中添加1μg·mL~(-1)β-蒎烯时，Xcc 29-1几乎不能生长。同时，0.1μg·mL~(-1)的β-蒎烯能有效在体内外诱导Ⅲ型分泌系统相关基因hrp G、hrp X、hrc V和hrp D6的转录表达。β-蒎烯作为柑橘的次级代谢产物可抑制Xcc 29-1的生长繁殖，抑菌作用的大小与其浓度正相关。与此同时，β-蒎烯能诱导Ⅲ型分泌系统相关基因的表达。说明β-蒎烯抑制的不是菌物的致病力，而是菌物的繁殖。
Keyword ：
hrp基因 hrp基因 β-蒎烯 β-蒎烯 抑制生长 抑制生长 柑橘溃疡病 柑橘溃疡病 诱导表达 诱导表达
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| GB/T 7714 | 叶子怡 , 范晓静 , 卓涛 et al. 柑橘次级代谢产物β-蒎烯对柑橘溃疡病菌的生长抑制作用与机理初探 [J]. | 武夷科学 , 2022 , 38 (01) : 25-30 . |
| MLA | 叶子怡 et al. "柑橘次级代谢产物β-蒎烯对柑橘溃疡病菌的生长抑制作用与机理初探" . | 武夷科学 38 . 01 (2022) : 25-30 . |
| APA | 叶子怡 , 范晓静 , 卓涛 , 邹华松 , 户勋 . 柑橘次级代谢产物β-蒎烯对柑橘溃疡病菌的生长抑制作用与机理初探 . | 武夷科学 , 2022 , 38 (01) , 25-30 . |
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青枯菌通过Ⅲ型分泌系统向寄主植物细胞分泌100多种效应蛋白，对寄主植物的抗感病性产生影响。青枯菌效应蛋白RipQ启动子区存在典型的HrpB识别序列PIP box (5′-TTCGG-N15-TTCGC-3′),但其功能尚未明确。本研究分析了RipQ在青枯菌4种演化型菌株中的分布情况。以青枯菌GMI1000为出发菌，构建ripQ缺失突变体和过表达菌株，研究效应蛋白RipQ在青枯菌-番茄植物互作中的功能。结果显示，ripQ广泛分布于除演化型IV的不同青枯菌类群中。与野生型菌株相比，ripQ突变体在番茄上的致病力有一定程度的增强，而ripQ过表达菌株的致病力显著降低。突变体和过表达菌株在培养基中的生长与野生型没有区别，但过表达菌株在番茄体内的繁殖能力下降。RipQ过表达菌株侵染番茄后hrpB、hrpG和epsA基因表达量显著下调，且能够诱导番茄叶片H_2O_2的大量累积，过敏性坏死反应标志基因hin1和水杨酸信号通路标志基因PR1a的诱导表达。另外，在番茄上瞬时表达ripQ也可以观察到H_2O_2积累及叶片细胞坏死，伴随着hin1和PR1a的上调表达。这些结果表明效应蛋白RipQ具有诱导番茄抗性的作用，从而影响青枯菌在番茄植物上的致病力。
Keyword ：
抗性 抗性 效应蛋白RipQ 效应蛋白RipQ 致病力 致病力 青枯菌 青枯菌
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| GB/T 7714 | 贵彩英 , TAHIRA Saleem , 谢文松 et al. 青枯菌GMI1000效应蛋白RipQ对致病力的作用研究 [J]. | 植物病理学报 , 2022 , 52 (05) : 801-812 . |
| MLA | 贵彩英 et al. "青枯菌GMI1000效应蛋白RipQ对致病力的作用研究" . | 植物病理学报 52 . 05 (2022) : 801-812 . |
| APA | 贵彩英 , TAHIRA Saleem , 谢文松 , 卓涛 , 户勋 , 邹华松 et al. 青枯菌GMI1000效应蛋白RipQ对致病力的作用研究 . | 植物病理学报 , 2022 , 52 (05) , 801-812 . |
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青枯模式菌株GMI1000分泌的Ⅲ型效应子RipI使致病力降低,靶定寄主植物转录因子bHLH诱导JA/ET抗病信号通路。通过预测发现RipI含有两个特殊的保守结构域,分别为整合酶结构域(58～174)和DNA聚合酶Ⅲ亚基γ和τ结构域(175～378)。酵母双杂交和荧光素酶互补实验结果显示,整合酶结构域与bHLH没有互作,但对RipⅠ与bHLH的互作有影响;DNA聚合酶Ⅲ结构域与RipⅠ存在互作关系。在本氏烟上瞬时表达RipI各个结构域发现,整合酶结构域和DNA聚合酶Ⅲ结构域单独都不能诱导细胞坏死,RipⅠ对细胞坏死的诱导需要这两个功能域共同存在。本文基于蛋白结构预测分析,研究了RipⅠ与bHLH互作及诱导植物细胞坏死的关键功能域,为认识效应子RipⅠ诱导植物抗性的分子机制提供了线索。
Keyword ：
效应蛋白RipⅠ 效应蛋白RipⅠ 转录因子bHLH93 转录因子bHLH93 青枯菌 青枯菌
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| GB/T 7714 | 赵同心 , 范晓静 , 邹华松 et al. 青枯菌Ⅲ型效应子RipI与寄主bHLH互作的关键结构域定位 [C] //植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . 2021 . |
| MLA | 赵同心 et al. "青枯菌Ⅲ型效应子RipI与寄主bHLH互作的关键结构域定位" 植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . (2021) . |
| APA | 赵同心 , 范晓静 , 邹华松 , 卓涛 . 青枯菌Ⅲ型效应子RipI与寄主bHLH互作的关键结构域定位 植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . (2021) . |
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柑橘溃疡病是由柑橘黄单胞杆菌[Xanthomonas subsp.citri (Xcc)]引起的一个重要细菌病害。此前研究发现Xcc 29-1菌株中的一个非典型反应调节因子VemR通过与σ54因子RpoN2互作共同调控鞭毛合成基因flgG的转录表达;同时证明了fleQ-vemR-rpoN2组成一个转录单元。本研究发现,在Xcc 29-1背景下构建缺失突变体ΔfleQ,对柑橘的致病力增强,胞外多糖的合成增加,在半固体培养基上的游动性降低。利用运用荧光定量Real-time PCR(qRTPCR)检测到ΔfleQ突变体中flgG的表达量下调;启动子融合GUS实验也证实ΔfleQ突变体中flgG启动子的活性下降,说明FleQ正调控鞭毛合成基因flgG的转录表达。FleQ和RpoN2能分别与VemR互作,但FleQ和RpoN2没有互作,说明三者之间是通过VemR的中间作用形成一个复合物,共同正调控基因flgG转录表达。
Keyword ：
fleQ fleQ flgG flgG VemR VemR 柑橘溃疡病菌 柑橘溃疡病菌 细胞游动性 细胞游动性 调控 调控
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| GB/T 7714 | 吴薇 , 赵续续 , 陈欣瑜 et al. 柑橋溃疡病菌FleQ参与调控鞭毛基因flgG的作用机制研究 [C] //植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . 2021 . |
| MLA | 吴薇 et al. "柑橋溃疡病菌FleQ参与调控鞭毛基因flgG的作用机制研究" 植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . (2021) . |
| APA | 吴薇 , 赵续续 , 陈欣瑜 , 邹华松 . 柑橋溃疡病菌FleQ参与调控鞭毛基因flgG的作用机制研究 植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . (2021) . |
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