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学者姓名：陈姗姗
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Abstract ：
Geobacter metallireducens GS-15中存在Gmet＿1513基因可编码具有自动诱导结合域的LuxR家族转录调控因子，使GS-15借助N-酰基高丝氨酸内酯促进产电及生物膜形成成为可能.而目前有关地杆菌群感效应的相关研究大多基于投加化学信号分子或信号分子淬灭剂的手段，缺乏靶向性与精确性.本研究构建了Geobacter metallireducens GS-15的Gmet＿1513基因缺失突变株（Δgmet＿1513），研究Gmet＿1513在GS-15菌株发挥电活性过程中的功能.实验结果表明，Δgmet＿1513与野生型菌株相比产生群感效应信号分子的能力减弱，Δgmet＿1513外膜蛋白种类减少，生物电化学系统的启动时间延长且电流输出减弱.与此同时，激光共聚焦图像对比结果表明Δgmet＿1513无法形成成熟致密电活性生物膜，EPS成分显示Δgmet＿1513的蛋白质含量低，多糖、脂质含量高，使其电子产量低且转移困难，无法进行AHLs的识别及结合导致电子传递相关蛋白无法表达，电子传递多重受阻.本研究为今后以Gmet＿1513基因为靶标，利用群感效应相关蛋白表达调控地杆菌生物膜形成以及产电提供依据.
Keyword ：
Gmet＿1513基因 Gmet＿1513基因 电化学活性 电化学活性 革兰氏阴性菌 革兰氏阴性菌 高丝氨酸内酯 高丝氨酸内酯
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| GB/T 7714 | 杨钰婷 , 靖宪月 , 陈姗姗 et al. 地杆菌中基因Gmet＿1513对其产电及生物膜形成的影响 [J]. | 环境科学学报 , 2023 , 43 (07) : 121-129 . |
| MLA | 杨钰婷 et al. "地杆菌中基因Gmet＿1513对其产电及生物膜形成的影响" . | 环境科学学报 43 . 07 (2023) : 121-129 . |
| APA | 杨钰婷 , 靖宪月 , 陈姗姗 , 刘星 , 周顺桂 . 地杆菌中基因Gmet＿1513对其产电及生物膜形成的影响 . | 环境科学学报 , 2023 , 43 (07) , 121-129 . |
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本发明公开了一种土壤原位固氮的方法，该固氮方法包括以下步骤：在土壤中添加电活性菌，并在三电极系统工作下进行原位固氮。本发明中的固氮方法通过在低氮土壤体系中接种电活性菌，构建三电极系统并施加工作电位，从而实现在土壤中原位固氮，降低经济成本，提高固氮效率，固氮过程中无污染物产生，对环境友好。此外，本发明通过在土壤中投入导电颗粒可进一步提高固氮效率，该方法操作便利，经济有效。
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| GB/T 7714 | 陈姗姗 , 韩昕桐 , 靖宪月 et al. 一种土壤原位固氮的方法 : CN202210230337.6[P]. | 2022-03-09 . |
| MLA | 陈姗姗 et al. "一种土壤原位固氮的方法" : CN202210230337.6. | 2022-03-09 . |
| APA | 陈姗姗 , 韩昕桐 , 靖宪月 , 周顺桂 . 一种土壤原位固氮的方法 : CN202210230337.6. | 2022-03-09 . |
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Natural hydroxyl radical (center dot OH) production, which partially occurs through the microbially driven Fenton reaction, can enhance the degradation of polystyrene microplastics (PS-MPs). However, center dot OH causes damage to microorganisms, which might in turn restrain the microbially driven Fenton reaction. Thus, whether PS-MPs can be continuously degraded by the microbially driven Fenton reaction and how they are degraded are still unknown. A pure-culture system using Shewanella putrefaciens 200 was set up to explore the effect and mechanism of microbially driven Fenton reaction on PS-MP degradation. In a 14-day operation, center dot OH produced by the microbially driven Fenton reaction could degrade PS-MPs with a weight loss of 6.1 +/- 0.6% and an O/C ratio of 0.6 (v.s. 0.6 +/- 0.1% and 0.1, respectively, in the center dot OH quenched group). Benzene ring derivatives such as 2-isopropyl-5-methyl-1-heptanol and nonahexacontanoic acid were the main soluble products of PS-MP degradation. The center dot OH-induced oxidative damage on microorganisms did not affect center dot OH production significantly when there was timely replenishment of organic carbon sources to promote reproduction of microorganisms. Thus, PS-MPs can be continuously degraded by microbially driven Fenton reactions in natural alternating anaerobic-aerobic environments. This study also provides a new microbial technique for MP degradation that is different from previous technologies based on microbial plastic-degrading enzymes.
Keyword ：
Fenton reaction Fenton reaction Hydroxyl radical Hydroxyl radical Iron-reducing bacterium Iron-reducing bacterium Microbial damage Microbial damage Polystyrene microplastic Polystyrene microplastic
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| GB/T 7714 | Yang, Yuting , Chen, Jin , Chen, Zhi et al. Mechanisms of polystyrene microplastic degradation by the microbially driven Fenton reaction [J]. | WATER RESEARCH , 2022 , 223 . |
| MLA | Yang, Yuting et al. "Mechanisms of polystyrene microplastic degradation by the microbially driven Fenton reaction" . | WATER RESEARCH 223 (2022) . |
| APA | Yang, Yuting , Chen, Jin , Chen, Zhi , Yu, Zhen , Xue, Jingchuan , Luan, Tiangang et al. Mechanisms of polystyrene microplastic degradation by the microbially driven Fenton reaction . | WATER RESEARCH , 2022 , 223 . |
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Diazotrophs can produce bioavailable nitrogen from inert N-2 gas by bioelectrochemical nitrogen fixation (e-BNF), which is emerging as an energy-saving and highly selective strategy for agriculture and industry. However, current e-BNF technology is impeded by requirements for NH4+ assimilation inhibitors to facilitate intracellular ammonia secretion and precious metal catalysts to generate H 2 as the energy-carrying intermediate. Here, we initially demonstrate inhibitor- and catalystless extracellular NH4+ production by the diazotroph Pseudomonas stutzeri A1501 using an electrode as the sole electron donor. Multiple lines of evidence revealed that P. stutzeri produced 232 +/- 0.25 mg/liter extracellular NH, at a poised potential of -0.3 V (versus standard hydrogen electrode [SHE)) without the addition of inhibitors or expensive catalysts. The electron uptake mechanism was attributed to the endogenous electron shuttle phenazine-1-carboxylic acid, which was excreted by P. stutzeri and mediated electron transfer from electrodes into cells to directly drive N-2 fixation. The faradaic efficiency was 20% = 3%, which was 2 to 4 times that of previous e-BNF attempts using the H-2-mediated pathway. This study reports a diazotroph capable of producing secretable NH4+ via extracellular electron uptake, which has important implications for optimizing the performance of e-BNF systems and exploring the novel nitrogen-fixing mode of syntrophic microbial communities in the natural environment. IMPORTANCE Ammonia greatly affects global ecology, agriculture, and the food industry. Diazotrophs with an enhanced capacity of extracellular NH4+ excretion have been proven to be more beneficial to the growth of microalgae and plants, whereas most previously reported diazotrophs produce intracellular organic nitrogen in the absence of chemical suppression and genetic manipulation. Here, we demonstrate that Pseudomonas stutzeri A1501 is capable of extracellular NH4+ production without chemical suppression or genetic manipulation when the extracellular electrode is used as the sole electron donor. We also reveal the electron uptake pathway from the extracellular electron-donating partner to P. stutzeri A1501 via redox electron shuttle phenazines. Since both P. stutzeri A1501 and potential electron-donating partners (such as electroactive microbes and natural semiconductor minerals) are abundant in diverse soils and sediments, P. stutzeri A1501 has broader implications on the improvement of nitrogen fertilization in the natural environment.
Keyword ：
bioelectrochemical system bioelectrochemical system electron uptake mechanism electron uptake mechanism nitrogen fixation nitrogen fixation phenazine phenazine Pseudomonas stutzeri Pseudomonas stutzeri
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| GB/T 7714 | Chen, Shanshan , Jing, Xianyue , Yan, Yongliang et al. Bioelectrochemical Fixation of Nitrogen to Extracellular Ammonium by Pseudomonas stutzeri [J]. | APPLIED AND ENVIRONMENTAL MICROBIOLOGY , 2021 , 87 (5) . |
| MLA | Chen, Shanshan et al. "Bioelectrochemical Fixation of Nitrogen to Extracellular Ammonium by Pseudomonas stutzeri" . | APPLIED AND ENVIRONMENTAL MICROBIOLOGY 87 . 5 (2021) . |
| APA | Chen, Shanshan , Jing, Xianyue , Yan, Yongliang , Huang, Shaofu , Liu, Xing , Chen, Piao et al. Bioelectrochemical Fixation of Nitrogen to Extracellular Ammonium by Pseudomonas stutzeri . | APPLIED AND ENVIRONMENTAL MICROBIOLOGY , 2021 , 87 (5) . |
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Aging of microplastics (MPs) (i.e., degradation and weathering) is ubiquitous in the environment. The MP aging process is thought to be limited to light and static areas, while aging in dark and fluctuating anoxic-oxic areas is poorly understood. Here, we provide initial evidence for aging of polystyrene microplastics (PS-MPs) under different anoxic/oxic conditions in sediments, and we further explored these mechanisms using sediment column experiments and pure-culture experiments. The results showed that PS-MPs in alternating anoxic-oxic sediments displayed the highest degree of aging. In the in-situ experiment, both the weight losses and O/C ratios of PS-MPs aged under alternating anoxic-oxic conditions were similar to 2 times higher than those aged under static oxic and static anoxic conditions during 2-month experiments. In a 2-month column experiment, the PS-MPs in the alternating anoxic-oxic group showed weight losses and O/C ratios that were, respectively, triple and double the corresponding values for the static oxic and static anoxic groups. Column and pure-culture experiments demonstrated that dark production of center dot OH which showed a positive correlation with a Fe redox process could explain enhanced MP aging under the alternating anoxic-oxic conditions. These findings provide a basis for risk assessment and management of MPs in the natural environment, such as in intertidal zones and paddy fields, and also have implications for engineering of optimized MP degradation processes.
Keyword ：
Aging degree Aging degree Alternating anoxic-oxic Alternating anoxic-oxic Fe-reducing bacteria Fe-reducing bacteria Hydroxyl radical Hydroxyl radical Microplastic Microplastic
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| GB/T 7714 | Chen, Shanshan , Yang, Yuting , Jing, Xianyue et al. Enhanced aging of polystyrene microplastics in sediments under alternating anoxic-oxic conditions [J]. | WATER RESEARCH , 2021 , 207 . |
| MLA | Chen, Shanshan et al. "Enhanced aging of polystyrene microplastics in sediments under alternating anoxic-oxic conditions" . | WATER RESEARCH 207 (2021) . |
| APA | Chen, Shanshan , Yang, Yuting , Jing, Xianyue , Zhang, Lanlan , Chen, Jin , Rensing, Christopher et al. Enhanced aging of polystyrene microplastics in sediments under alternating anoxic-oxic conditions . | WATER RESEARCH , 2021 , 207 . |
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Bacteria in biofilms are able to utilize potassium ion channel-mediated electrical signaling to achieve cell-cell communication. However, it remains unclear whether these signals play a role in Geobacter sp. when surrounded by an intense electric field. This study used a potassium channel blocker (tetraethylammonium, TEA) that interfered with the release of K' but not bacterial growth to demonstrate that potassium ion channel-mediated electrical signaling affected the formation and electroactivity of Geobacter sulfurreducens. The results showed that 5 mM TEA slowed the formation of Geobacter sulfurreducens biofilm, and the current density was -50% lower than in the control. The electrochemical analyses showed that the electroactivity of the biofilms with TEA addition was inferior. In particular, the micrometer- scale biofilm with TEA exhibited fewer high current peaks, and the species of outermost groups that participated in the electron transfer in Geobacter sulfurreducens biofilms was different from the control. This work provides initial evidence to reveal the role of potassium channels in Geobacter sulfurreducens electroactive biofilms. (C) 2019 Elsevier B.V. All rights reserved.
Keyword ：
Biofilm formation Biofilm formation Electrical signaling Electrical signaling Electroactivity Electroactivity Geobacter sulfurreducens Geobacter sulfurreducens Potassium channel blocker Potassium channel blocker
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| GB/T 7714 | Jing, Xianyue , Yang, Yuting , Ai, Zhihao et al. Potassium channel blocker inhibits the formation and electroactivity of Geobacter biofilm [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2020 , 705 . |
| MLA | Jing, Xianyue et al. "Potassium channel blocker inhibits the formation and electroactivity of Geobacter biofilm" . | SCIENCE OF THE TOTAL ENVIRONMENT 705 (2020) . |
| APA | Jing, Xianyue , Yang, Yuting , Ai, Zhihao , Chen, Shanshan , Zhou, Shungui . Potassium channel blocker inhibits the formation and electroactivity of Geobacter biofilm . | SCIENCE OF THE TOTAL ENVIRONMENT , 2020 , 705 . |
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群感效应(quorum sensing,QS)是微生物之间以信号分子受体蛋白感知信号浓度变化,从而调控菌群的行为及功能,使其适应环境变化的信号通讯机制。电活性微生物(electroactive microorganisms,EAMs)能进行胞外电子传递,在可再生能源利用和环境修复方面具有广阔的应用前景。近年来,关于QS在EAMs胞外电子传递中的作用的研究日益增多。本文总结了QS对纯EAMs或混合产电菌群的直接或间接电子传递的影响效应及机制,阐述了基于QS的EAMs逻辑与门的构建及其应用前景,并从机制研究的角度展望其未来发展方向。
Keyword ：
信号分子 信号分子 电活性微生物 电活性微生物 群感效应 群感效应 胞外呼吸 胞外呼吸 逻辑与门 逻辑与门
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| GB/T 7714 | 杨钰婷 , 陈瑾 , 陈姗姗 et al. 群感效应对电活性微生物胞外电子传递的影响 [J]. | 微生物学报 , 2020 , 60 (11) : 2399-2411 . |
| MLA | 杨钰婷 et al. "群感效应对电活性微生物胞外电子传递的影响" . | 微生物学报 60 . 11 (2020) : 2399-2411 . |
| APA | 杨钰婷 , 陈瑾 , 陈姗姗 , 周顺桂 . 群感效应对电活性微生物胞外电子传递的影响 . | 微生物学报 , 2020 , 60 (11) , 2399-2411 . |
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Biofilm formation and maturation have been demonstrated to be regulated by distinct forms of cell-cell communication factors such as chemical and physical signals. However, whether the Geobacter sp. biofilms, which are typical electroactive biofilms, are affected by chemical signals is poorly understood. This research investigated the effects and corresponding mechanisms of endogenous and exogenous chemical signals (i.e., Nacylhamoserine lactones, AHLs) on the Geobacter soli biofilm. The results showed that Geobacter soli GSS01 secreted detectable endogenous AHLs to facilitate the formation and electrochemical activity of the biofilm, and that exogenous AHLs could further promoted these activities. Analyses of surface proteins revealed that the mechanisms promoted by endogenous and exogenous AHLs were somewhat different. Endogenous AHLs improved the relative abundance of external membrane proteins, while exogenous AHLs further facilitated the formation of amide II and a stronger H-bond between the carbonyl group and the amide. Furthermore, the proteomics analysis indicated that endogenous AHLs enhanced extracellular polymeric substance production by up-regulating the expression of key enzymes participating in EPS production, and simultaneously affected the physiological performance of individual cells. These results demonstrate, for the first time, the importance of chemical signals in Geobacter sp. and provide a comprehensive understanding of the chemical signals involved in biofilm formation and electrochemical activity of Geobacter sp..
Keyword ：
Acylhomoserine lactone Acylhomoserine lactone Biofilm formation Biofilm formation Chemical signal Chemical signal Electroactivity Electroactivity Geobacter soli Geobacter soli
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| GB/T 7714 | Jing, Xianyue , Liu, Xing , Deng, Chengsheng et al. Chemical signals stimulate Geobacter soli biofilm formation and electroactivity [J]. | BIOSENSORS & BIOELECTRONICS , 2019 , 127 : 1-9 . |
| MLA | Jing, Xianyue et al. "Chemical signals stimulate Geobacter soli biofilm formation and electroactivity" . | BIOSENSORS & BIOELECTRONICS 127 (2019) : 1-9 . |
| APA | Jing, Xianyue , Liu, Xing , Deng, Chengsheng , Chen, Shanshan , Zhou, Shungui . Chemical signals stimulate Geobacter soli biofilm formation and electroactivity . | BIOSENSORS & BIOELECTRONICS , 2019 , 127 , 1-9 . |
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To date, there is a general lack of research devoted to light-semiconductive nanoparticle (NP)-syntrophy systems though they are ubiquitous in the earth's near-surface. This study demonstrates for the first time that visible light repressed Geobacter co-cultures, whereas light-excited CdS NPs could alleviate this photo-induced stress. Light-excited CdS NPs generated photoelectron-hole pairs to drive direct interspecies electron transfer (DIET) rather than acting as shades or electrical conduits. The mutant studies and RT-qPCR data revealed that the photoelectron-driven DIET needed involvements of cytochromes Gmet_2896 and OmcS, but required fewer of these two key cytochromes than the systems without CdS NPs. Thus, energy consumption for cytochrome biosynthesis was reduced and the photo-induced stress was alleviated. These findings provide a new mechanistic model of DIET between electron-donating and electron-accepting partners, and broaden the knowledge of near-surface biogeochemical processes in nature and interfacial reactions in photocatalytic biotechnologies.
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| GB/T 7714 | Chen, Shanshan , Deng, Chengsheng , Liu, Xing et al. CdS nanoparticles alleviate photo-induced stress in Geobacter co-cultures [J]. | ENVIRONMENTAL SCIENCE-NANO , 2019 , 6 (6) : 1941-1949 . |
| MLA | Chen, Shanshan et al. "CdS nanoparticles alleviate photo-induced stress in Geobacter co-cultures" . | ENVIRONMENTAL SCIENCE-NANO 6 . 6 (2019) : 1941-1949 . |
| APA | Chen, Shanshan , Deng, Chengsheng , Liu, Xing , Yang, Yuting , Cai, Xixi , Huang, Haibo et al. CdS nanoparticles alleviate photo-induced stress in Geobacter co-cultures . | ENVIRONMENTAL SCIENCE-NANO , 2019 , 6 (6) , 1941-1949 . |
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The extracellular electron transfer (EET) efficiency in bioelectrochemical systems has been proven to be dependent on anode potentials. To explore the underlying mechanism, previous studies have mainly focused on EET conduit and bacterial biomass but rarely concerned with the role of extracellular polymeric substances (EPS) surrounding electroactive cells. In this study, the response of Geobacter biofilms to anode potentials was investigated with a special emphasis on the mechanistic role of EPS. The electrochemical activities and cell viabilities of Geobacter soli biofilms were simultaneously attenuated at 0.4 and 0.6 V compared to -0.2 and 0 V. It was found that the biofilms (especially the biofilm region closer to electrode surface) grown at -0.2 and 0 V produced relatively more extracellular redox-active proteins and less extracellular polysaccharides, which conferred higher electron accepting/donating capacities to EPS and consequently facilitated EET. Meanwhile, electrically nonconductive extracellular polysaccharide-dominated interior layers were formed in the biofilms grown at 0.4 and 0.6 V, which limited direct EET but might serve as physical barriers for protecting cells in these biofilms from the increasing stress by poised electrodes. These results demonstrated that the production of EPS under different anode potentials might be finely regulated by cells to keep balance between EET efficiency and cell-protection. This study provides a new insight to investigate the Geobacter biofilms coping with various environments, and is useful for optimizing electrochemical activity of anode biofilms. (C) 2019 Elsevier Ltd. All rights reserved.
Keyword ：
Anode potentials Anode potentials Bioelectrochemical system Bioelectrochemical system Extracellular electron transfer Extracellular electron transfer Extracellular polymeric substances Extracellular polymeric substances Geobacter soli Geobacter soli
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| GB/T 7714 | Yang, Guiqin , Huang, Lingyan , Yu, Zhen et al. Anode potentials regulate Geobacter biofilms: New insights from the composition and spatial structure of extracellular polymeric substances [J]. | WATER RESEARCH , 2019 , 159 : 294-301 . |
| MLA | Yang, Guiqin et al. "Anode potentials regulate Geobacter biofilms: New insights from the composition and spatial structure of extracellular polymeric substances" . | WATER RESEARCH 159 (2019) : 294-301 . |
| APA | Yang, Guiqin , Huang, Lingyan , Yu, Zhen , Liu, Xiaoming , Chen, Shanshan , Zeng, Jianxiong et al. Anode potentials regulate Geobacter biofilms: New insights from the composition and spatial structure of extracellular polymeric substances . | WATER RESEARCH , 2019 , 159 , 294-301 . |
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