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学者姓名：岳川
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Abstract ：
以不同茶树（Camellia sinensis）基因组为参考，通过序列同源性分析，在‘龙井43’中鉴定并克隆到3个CsIDM基因。CsIDM1属于组蛋白乙酰转移酶家族，具有典型的HAT保守结构域，定位于细胞核中。CsIDM2、CsIDM3均属于热激蛋白家族，具有典型的ACD保守结构域，均定位于细胞核、细胞膜及细胞质中。CsIDM启动子区域含有多种响应环境信号的顺式作用元件，主要为光响应、植物激素响应、胁迫响应和植物生长发育相关。CsIDM在花蕾、腋芽、茎中表达量较高，在盛花组织中较低。在干旱、高温及生物胁迫下，CsIDM均出现不同程度的差异表达。茶树越冬芽休眠形成与解除过程中，CsIDM1的表达丰度在休眠形成、深休眠和休眠解除3个阶段存在高—低—高的表达模式。通过双分子荧光互补试验证实，CsIDM2和CsIDM3之间可形成异源二聚体；CsIDM2与Cs MBD5、CsIDM3与CsMBD16存在相互作用。综上所述，CsIDM在茶树的胁迫应答和芽休眠解除中发挥作用，可能通过形成蛋白复合体参与甲基化调控。
Keyword ：
BiFC互作 BiFC互作 IDM复合物 IDM复合物 亚细胞定位 亚细胞定位 去甲基化 去甲基化 茶树 茶树 表达分析 表达分析
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| GB/T 7714 | 李宇腾 , 陈瑶 , 任恒泽 et al. 茶树CsIDM的鉴定、表达分析及互作验证 [J]. | 园艺学报 , 2023 , 50 (08) : 1679-1696 . |
| MLA | 李宇腾 et al. "茶树CsIDM的鉴定、表达分析及互作验证" . | 园艺学报 50 . 08 (2023) : 1679-1696 . |
| APA | 李宇腾 , 陈瑶 , 任恒泽 , 李聪聪 , 王浩乾 , 曹红利 et al. 茶树CsIDM的鉴定、表达分析及互作验证 . | 园艺学报 , 2023 , 50 (08) , 1679-1696 . |
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金线莲以其较高的营养价值与药理作用而受到重视，但其特有却并不令人喜爱的滋味极大地限制了它在食品工业领域的应用。该研究拟通过复配其他花草茶原料来改善金线莲固有的不良滋味特征，以期在充分利用其功能活性的同时获得更佳的风味品质。使用9种花草茶原料、21种原料配比，以感官品质、功能成分含量以及抗氧化活性为评价指标分析产品的综合品质。结果表明，金线莲与茶叶、夏枯球等成分复配不仅可以改善其感官品质还有利于其功能活性的最大化发挥，基于此，通过不同原料复配发现，金线莲∶白茶∶陈皮=10∶2∶3（质量比）与夏枯球∶白茶∶金线莲∶陈皮∶金花茶=4∶3∶6∶5∶5（质量比）是较优配方。该配方下金线莲的功能成分含量与抗氧化活性均保持在较优水平。该研究为改善金线莲固有的不良滋味特征提供思路，对金线莲在食品工业领域的广泛应用具有实践指导意义。
Keyword ：
功能成分 功能成分 复配 复配 感官品质 感官品质 抗氧化活性 抗氧化活性 花草茶 花草茶 金线莲 金线莲
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| GB/T 7714 | 高一舟 , 汪芳 , 曹青青 et al. 不同原料复配组成对金线莲花草茶品质的影响 [J]. | 食品研究与开发 , 2023 , 44 (13) : 90-96 . |
| MLA | 高一舟 et al. "不同原料复配组成对金线莲花草茶品质的影响" . | 食品研究与开发 44 . 13 (2023) : 90-96 . |
| APA | 高一舟 , 汪芳 , 曹青青 , 岳川 , 吴梅 , 许勇泉 et al. 不同原料复配组成对金线莲花草茶品质的影响 . | 食品研究与开发 , 2023 , 44 (13) , 90-96 . |
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激素在植物生长发育调控中发挥重要作用，为明确不同激素对茶树新梢生长发育的影响，挖掘参与调控这一过程的主要通路和关键基因，以龙井43为试材，在茶树处于萌动期时分别进行100μmol·L~(-1)脱落酸（ABA）、100μmol·L~(-1)赤霉素（GA_3）和100μmol·L~(-1)吲哚-3-乙酸（IAA）喷施处理，观察新梢萌发表型，并对处理后第7天的新梢进行转录组测序分析。结果显示，外源ABA处理抑制新梢生长，处理7 d后芽长极显著短于对照；GA_3和IAA处理则具有促进作用，GA_3处理7 d芽长极显著长于对照，IAA处理14 d芽长极显著长于对照。转录组分析表明，ABA处理新梢中的氨基酸生物合成通路、GA_3处理的氧化磷酸化通路和光合作用通路及IAA处理的类黄酮生物合成通路是差异基因富集的主要通路；植物激素通路、光合作用通路相关的GAI、PSBO2、PSBQ-2和PSBP-1可能是参与新梢生长发育的关键基因。对部分候选基因的表达模式进行实时荧光定量验证，表明转录组分析结果可靠。以上研究明确了激素影响茶树新梢生长发育的主要通路和关键基因，将为全面揭示茶树新梢生长发育调控机制提供理论依据。
Keyword ：
吲哚-3-乙酸 吲哚-3-乙酸 生长发育 生长发育 脱落酸 脱落酸 茶树 茶树 赤霉素 赤霉素 转录组 转录组
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| GB/T 7714 | 李聪聪 , 王浩乾 , 叶玙璠 et al. 植物激素对茶树春季新梢生长发育的调控作用研究 [J]. | 茶叶科学 , 2023 , 43 (03) : 335-348 . |
| MLA | 李聪聪 et al. "植物激素对茶树春季新梢生长发育的调控作用研究" . | 茶叶科学 43 . 03 (2023) : 335-348 . |
| APA | 李聪聪 , 王浩乾 , 叶玙璠 , 陈瑶 , 任恒泽 , 李宇腾 et al. 植物激素对茶树春季新梢生长发育的调控作用研究 . | 茶叶科学 , 2023 , 43 (03) , 335-348 . |
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摇青是乌龙茶品质形成的关键工序之一，其通过机械胁迫引起各种生物学效应，影响植物体内一系列生理生化变化，被广泛应用于花香红茶、花香白茶、花香绿茶等茶类的加工中以改善品质。为探明青叶响应机械力胁迫的生理变化，以采摘的‘铁观音’品种中开面三、四叶嫩梢为材料，经适度萎凋后进行自然摊放和振动力胁迫处理，研究青叶水分含量、光系统Ⅱ(photosystem Ⅱ,PS Ⅱ)参数、抗氧化酶活力及亚细胞结构的变化规律。结果表明：振动力胁迫30 min内青叶水分含量与自然摊放叶、萎凋叶间无显著差异（P>0.05）,60 min时显著低于自然摊放叶和萎凋叶（P<0.05）。随着振动力胁迫时间延长，青叶青臭气增强，清香减弱；叶绿体和类囊体紧密而清晰的片层结构逐渐松散、变形、收缩、紊乱、瓦解，振动力胁迫处理5 min时淀粉粒数量明显增加，15 min时嗜锇颗粒数量明显增多，出现聚集。振动力胁迫青叶的PS Ⅱ实际光合效率Y(Ⅱ)和光化学猝灭系数qP随处理时间延长总体呈下降趋势，电子传递速率（electron transfer rate,ETR）随处理时间延长呈先降低后升高趋势，5 min和10 min时PS Ⅱ Y(Ⅱ)和ETR显著低于自然摊放（P<0.05）；非调节性能量耗散的量子产额Y(NO)随振动力胁迫处理时间延长呈上升趋势，30 min时显著高于自然摊放叶（P<0.05）。振动力胁迫10 min青叶相对电导率显著高于自然摊放叶（P<0.05）；青叶抗氧化酶中抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶活力在振动力胁迫处理5、10 min时高于自然摊放叶。综上，一定时间（30 min以内）的振动力胁迫对青叶水分含量影响不显著，但青气变浓，加剧青叶细胞膜系统损伤，使其透性提高，促进膜内可溶物渗出，造成相对电导率升高，类囊体降解，叶绿体严重受损，PS Ⅱ反应中心被破坏；此外，振动力胁迫能够通过提高活性氧代谢酶活性抵御机械胁迫对叶细胞的伤害。本研究可为乌龙茶摇青品质调控提供参考。
Keyword ：
乌龙茶 乌龙茶 亚显微结构 亚显微结构 光系统Ⅱ参数 光系统Ⅱ参数 抗氧化酶 抗氧化酶 非生物胁迫 非生物胁迫
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| GB/T 7714 | 郝志龙 , 林宏政 , 徐邢燕 et al. 采后茶青叶对振动力胁迫的生理响应 [J]. | 食品科学 , 2023 , 44 (01) : 30-37 . |
| MLA | 郝志龙 et al. "采后茶青叶对振动力胁迫的生理响应" . | 食品科学 44 . 01 (2023) : 30-37 . |
| APA | 郝志龙 , 林宏政 , 徐邢燕 , 李鑫磊 , 俞晓敏 , 岳川 et al. 采后茶青叶对振动力胁迫的生理响应 . | 食品科学 , 2023 , 44 (01) , 30-37 . |
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Abstract ：
摇青是乌龙茶品质形成的关键工序之一,其通过机械胁迫引起各种生物学效应,影响植物体内一系列生理生化变化,被广泛应用于花香红茶、花香白茶、花香绿茶等茶类的加工中以改善品质。为探明青叶响应机械力胁迫的生理变化,以采摘的‘铁观音’品种中开面三、四叶嫩梢为材料,经适度萎凋后进行自然摊放和振动力胁迫处理,研究青叶水分含量、光系统Ⅱ(photosystem Ⅱ,PS Ⅱ)参数、抗氧化酶活力及亚细胞结构的变化规律。结果表明:振动力胁迫30 min内青叶水分含量与自然摊放叶、萎凋叶间无显著差异(P>0.05),60 min时显著低于自然摊放叶和萎凋叶(P<0.05)。随着振动力胁迫时间延长,青叶青臭气增强,清香减弱;叶绿体和类囊体紧密而清晰的片层结构逐渐松散、变形、收缩、紊乱、瓦解,振动力胁迫处理5 min时淀粉粒数量明显增加,15 min时嗜锇颗粒数量明显增多,出现聚集。振动力胁迫青叶的PS Ⅱ实际光合效率Y(Ⅱ)和光化学猝灭系数qP随处理时间延长总体呈下降趋势,电子传递速率(electron transfer rate,ETR)随处理时间延长呈先降低后升高趋势,5 min和10 min时PS Ⅱ Y(Ⅱ)和ETR显著低于自然摊放(P<0.05);非调节性能量耗散的量子产额Y(NO)随振动力胁迫处理时间延长呈上升趋势,30 min时显著高于自然摊放叶(P<0.05)。振动力胁迫10 min青叶相对电导率显著高于自然摊放叶(P<0.05);青叶抗氧化酶中抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶活力在振动力胁迫处理5、10 min时高于自然摊放叶。综上,一定时间(30 min以内)的振动力胁迫对青叶水分含量影响不显著,但青气变浓,加剧青叶细胞膜系统损伤,使其透性提高,促进膜内可溶物渗出,造成相对电导率升高,类囊体降解,叶绿体严重受损,PS Ⅱ反应中心被破坏;此外,振动力胁迫能够通过提高活性氧代谢酶活性抵御机械胁迫对叶细胞的伤害。本研究可为乌龙茶摇青品质调控提供参考。
Keyword ：
乌龙茶 乌龙茶 亚显微结构 亚显微结构 光系统Ⅱ参数 光系统Ⅱ参数 抗氧化酶 抗氧化酶 非生物胁迫 非生物胁迫
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| GB/T 7714 | Hao, Zhilong , Lin, Hongzheng , Xu, Xingyan et al. 采后茶青叶对振动力胁迫的生理响应 [J]. | 食品科学 , 2023 , 44 (1) : 30-37 . |
| MLA | Hao, Zhilong et al. "采后茶青叶对振动力胁迫的生理响应" . | 食品科学 44 . 1 (2023) : 30-37 . |
| APA | Hao, Zhilong , Lin, Hongzheng , Xu, Xingyan , Li, Xinlei , Yu, Xiaomin , Yue, Chuan et al. 采后茶青叶对振动力胁迫的生理响应 . | 食品科学 , 2023 , 44 (1) , 30-37 . |
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【目的】探究在茶树不同生育时期叶面喷施不同硒肥对夏茶产量、品质及硒含量的影响，为生产富硒茶提供技术依据。【方法】田间试验在浙江嵊州进行，供试茶树品种为‘中茶108’。试验采用二因素列区设计，主处理为硒肥种类(A因素)，副处理为硒肥喷施时期(B因素)。主处理设喷施清水对照(A0)、硒酸钠(A1)、亚硒酸钠(A2)和酵母硒(A3)；副处理设夏茶顶芽萌发前(5月12日，B1)与1芽1叶期(5月20日，B2)两个喷施时期。硒肥喷施浓度均为Se 50 mg/L，硒肥溶液喷施量为1.8 L/m2。当茶树蓬面1芽2叶占比达30%左右时，每个小区随机选取30 cm×30 cm茶蓬，调查蓬面新梢总数、1芽2叶数量、1芽2叶长度和百芽重。同时，取1芽2叶新梢样品，测定茶多酚、儿茶素、咖啡碱、游离氨基酸、花青素以及硒含量。【结果】与A0B1处理相比，A3B1处理茶树萌展值显著降低了0.14，但对茶树蓬面新梢总数无明显影响；A2B1处理茶树1芽2叶新梢长度和百芽重分别显著降低了1.04 cm和1.94 g。A1B2和A3B2处理茶树蓬面新梢总数分别显著降低了17.66和22.33，但不影响其萌展值；A1B2显著降低茶树1芽2叶新梢长度和百芽重，分别降低0.88 cm和1.70g。A1B1和A2B1处理的茶叶总硒含量分别显著提高了1.15和1.47 mg/kg，有机硒含量分别显著提高了1.13和1.38 mg/kg，但A3B1处理未显著提高茶叶硒含量。A1B2、A2B2和A3B2处理均显著提高了茶叶总硒和有机硒含量，其中总硒增加量分别为5.97、7.88和2.61 mg/kg，有机硒含量分别增加了5.17、7.51和2.48 mg/kg。另外，A1B1显著降低了茶叶咖啡碱、没食子酸和儿茶素含量；A1B2处理显著降低了茶叶游离氨基酸含量，但显著增加了茶多酚和儿茶素总量，儿茶素组成中的没食子酸、表没食子儿茶素没食子酸酯和表儿茶素没食子酸酯等酯型儿茶素显著增加。【结论】夏茶萌芽前喷施外源硒能够提高茶叶总硒和有机硒含量，改善茶叶品质，尤以硒酸钠的效果最好。
Keyword ：
亚硒酸钠 亚硒酸钠 喷施时期 喷施时期 硒含量 硒含量 硒残留 硒残留 硒酸钠 硒酸钠 茶叶 茶叶 茶叶品质 茶叶品质 酵母硒 酵母硒
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| GB/T 7714 | 李晓嫚 , 郭丽娜 , 郝心愿 et al. 萌芽前叶面喷施硒酸钠提高茶叶含硒量及品质 [J]. | 植物营养与肥料学报 , 2022 , 28 (10) : 1884-1892 . |
| MLA | 李晓嫚 et al. "萌芽前叶面喷施硒酸钠提高茶叶含硒量及品质" . | 植物营养与肥料学报 28 . 10 (2022) : 1884-1892 . |
| APA | 李晓嫚 , 郭丽娜 , 郝心愿 , 王璐 , 王月琪 , 王新超 et al. 萌芽前叶面喷施硒酸钠提高茶叶含硒量及品质 . | 植物营养与肥料学报 , 2022 , 28 (10) , 1884-1892 . |
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Tea varieties with specific colours have often been studied by researchers. However, previous studies on the albinism of tea plants have mostly been based on plants with different genetic backgrounds or focused on common components in albino tea leaves, such as amino acids, flavones, and carotenoids. In this study, we conducted widely targeted metabolic and transcriptomic analyses between a wildtype tea genotype (Shuixian, LS) and its albino mutant (Huangjinshuixian, HS). At the molecular level, alteration of gene expression levels in the MEP pathway may have reduced the production of chlorophyll and carotenoids in HS, which could be the main cause of the phenotypic changes in HS. At the metabolite level, a large number of metabolites related to light protection that significantly accumulated in HS, including flavones, anthocyanins, flavonols, flavanones, vitamins and their derivatives, polyphenols, phenolamides. This result, combined with an enzyme activity experiment, suggested that the absence of photosynthetic pigments made the albino tea leaves of HS more vulnerable to UV stress, even under normal light conditions. In addition, except for the common amino acids, we also identified numerous nitrogen-containing compounds, including nucleotides and their derivates, amino acid derivatives, glycerophospholipids, and phenolamides, which implied that significant accumulation of NH4+ in albino tea leaves could not only promote amino acid synthesis but could also activate other specialized metabolic pathways related to nitrogen metabolism. In conclusion, our results provide new information to guide further studies of the extensive metabolic reprogramming events caused by albinism in tea plants.
Keyword ：
Albino tea Albino tea Colour change Colour change Metabolomics Metabolomics Photoprotection Photoprotection Transcriptome Transcriptome
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| GB/T 7714 | Zheng, Yucheng , Wang, Pengjie , Chen, Xuejin et al. Integrated transcriptomics and metabolomics provide novel insight into changes in specialized metabolites in an albino tea cultivar (Camellia sinensis (L.) O. Kuntz) [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2021 , 160 : 27-36 . |
| MLA | Zheng, Yucheng et al. "Integrated transcriptomics and metabolomics provide novel insight into changes in specialized metabolites in an albino tea cultivar (Camellia sinensis (L.) O. Kuntz)" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 160 (2021) : 27-36 . |
| APA | Zheng, Yucheng , Wang, Pengjie , Chen, Xuejin , Yue, Chuan , Guo, Yongchun , Yang, Jiangfan et al. Integrated transcriptomics and metabolomics provide novel insight into changes in specialized metabolites in an albino tea cultivar (Camellia sinensis (L.) O. Kuntz) . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2021 , 160 , 27-36 . |
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Plants have evolved regulatory mechanisms at multiple levels to regulate gene expression in order to improve their cold adaptability. However, limited information is available regarding the stress response at the chromatin and translational levels. Here, we characterize the chromatin accessibility, transcriptional, and translational landscapes of tea plants in vivo under chilling stress for the first time. Chilling stress significantly affected both the transcription and translation levels as well as the translation efficiency of tea plants. A total of 3010 genes that underwent rapid and independent translation under chilling stress were observed, and they were significantly enriched in the photosynthesis-antenna protein and phenylpropanoid biosynthesis pathways. A set of genes that were significantly responsive to cold at the transcription and translation levels, including four (+)-neomenthol dehydrogenases (MNDs) and two (E)-nerolidol synthases (NESs) arranged in tandem on the chromosomes, were also found. We detected potential upstream open reading frames (uORFs) on 3082 genes and found that tea plants may inhibit the overall expression of genes by enhancing the translation of uORFs under chilling stress. In addition, we identified distal transposase hypersensitive sites (THSs) and proximal THSs and constructed a transcriptional regulatory network for tea plants under chilling stress. We also identified 13 high-confidence transcription factors (TFs) that may play a crucial role in cold regulation. These results provide valuable information regarding the potential transcriptional regulatory network in plants and help to clarify how plants exhibit flexible responses to chilling stress.
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| GB/T 7714 | Wang, Pengjie , Jin, Shan , Chen, Xuejin et al. Chromatin accessibility and translational landscapes of tea plants under chilling stress [J]. | HORTICULTURE RESEARCH , 2021 , 8 (1) . |
| MLA | Wang, Pengjie et al. "Chromatin accessibility and translational landscapes of tea plants under chilling stress" . | HORTICULTURE RESEARCH 8 . 1 (2021) . |
| APA | Wang, Pengjie , Jin, Shan , Chen, Xuejin , Wu, Liangyu , Zheng, Yucheng , Yue, Chuan et al. Chromatin accessibility and translational landscapes of tea plants under chilling stress . | HORTICULTURE RESEARCH , 2021 , 8 (1) . |
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Tea plants (Camellia sinensis) are commercially cultivated in >60 countries, and their fresh leaves are processed into tea, which is the most widely consumed beverage in the world. Although several chromosome-level tea plant genomes have been published, they collapsed the two haplotypes and ignored a large number of allelic variations that may underlie important biological functions in this species. Here, we present a phased chromosome-scale assembly for an elite oolong tea cultivar, "Huangdan", that is well known for its high levels of aroma. Based on the two sets of haplotype genome data, we identified numerous genetic variations and a substantial proportion of allelic imbalance related to important traits, including aroma- and stress-related alleles. Comparative genomics revealed extensive structural variations as well as expansion of some gene families, such as terpene synthases (TPSs), that likely contribute to the high-aroma characteristics of the backbone parent, underlying the molecular basis for the biosynthesis of aroma-related chemicals in oolong tea. Our results uncovered the genetic basis of special features of this oolong tea cultivar, providing fundamental genomic resources to study evolution and domestication for the economically important tea crop.
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| GB/T 7714 | Wang, Pengjie , Yu, Jiaxin , Jin, Shan et al. Genetic basis of high aroma and stress tolerance in the oolong tea cultivar genome [J]. | HORTICULTURE RESEARCH , 2021 , 8 (1) . |
| MLA | Wang, Pengjie et al. "Genetic basis of high aroma and stress tolerance in the oolong tea cultivar genome" . | HORTICULTURE RESEARCH 8 . 1 (2021) . |
| APA | Wang, Pengjie , Yu, Jiaxin , Jin, Shan , Chen, Shuai , Yue, Chuan , Wang, Wenling et al. Genetic basis of high aroma and stress tolerance in the oolong tea cultivar genome . | HORTICULTURE RESEARCH , 2021 , 8 (1) . |
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Abstract ：
Plants have evolved regulatory mechanisms at multiple levels to regulate gene expression in order to improve their cold adaptability.However,limited information is available regarding the stress response at the chromatin and translational levels.Here,we characterize the chromatin accessibility,transcriptional,and translational landscapes of tea plants in vivo under chilling stress for the first time.Chilling stress significantly affected both the transcription and translation levels as well as the translation efficiency of tea plants.A total of 3010 genes that underwent rapid and independent translation under chilling stress were observed,and they were significantly enriched in the photosynthesis-antenna protein and phenylpropanoid biosynthesis pathways.A set of genes that were significantly responsive to cold at the transcription and translation levels,including four(+)-neomentholdehydrogenases(MNDs)and two(E)-nerolidolsynthases(NESs)arranged in tandem on the chromosomes,were also found.We detected potential upstream open reading frames(uORFs)on 3082 genes and found that tea plants may inhibit the overall expression of genes by enhancing the translation of uORFs under chilling stress.In addition,we identified distal transposase hypersensitive sites(THSs)and proximal THSs and constructed a transcriptional regulatory network for tea plants under chilling stress.We also identified 13 high-confidence transcription factors(TFs)that may play a crucial role in cold regulation.These results provide valuable information regarding the potential transcriptional regulatory network in plants and help to clarify how plants exhibit flexible responses to chilling stress.
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| GB/T 7714 | Pengjie Wang , Shan Jin , Xuejin Chen et al. Chromatin accessibility and translational landscapes of tea plants under chilling stress [J]. | 园艺研究（英文） , 2021 , (1) : 1273-1287 . |
| MLA | Pengjie Wang et al. "Chromatin accessibility and translational landscapes of tea plants under chilling stress" . | 园艺研究（英文） 1 (2021) : 1273-1287 . |
| APA | Pengjie Wang , Shan Jin , Xuejin Chen , Liangyu Wu , Yucheng Zheng , Chuan Yue et al. Chromatin accessibility and translational landscapes of tea plants under chilling stress . | 园艺研究（英文） , 2021 , (1) , 1273-1287 . |
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