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学者姓名：方绍明
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Simple Summary Due to the fact that rabbit meat possesses high levels of proteins and polyunsaturated fatty acids with low contents of fat and cholesterol that can meet the urgent demand for a healthy diet in modern life, the meat rabbit industry in China has been rapidly developed in recent years. However, rabbits are more sensitive to seasonal changes owing to their specific physiological characteristics, such as few sweat glands, thick coats of fur, high metabolic rate, and high growth rate. In this study, we investigated the variations in productivity, health status, and gut microbiota of meat rabbits reared in semi-confined conditions between summer and winter. In the summer season, the relative high ambient temperature and humidity could trigger disturbance of the gut microbiome, potential heat stress, reduced antioxidant defense, and increased inflammation risk, which consequently deteriorated production performance. In the winter season, changes in energy demand, photoperiod, and feeding pattern should be regarded as important factors that affect the productivity of meat rabbits. Based on our findings, we not only proposed several realistic strategies to alleviate the unfavorable influences of seasonal alterations on the productivity and well-being of meat rabbits but also pointed out the future directions for this study of molecular mechanisms in adaptation physiology.Abstract In this study, we investigated the variations in production performance, health status, and gut microbiota of meat rabbits raised in the semi-confined barn during summer and winter. Compared to summer, rabbits reared in winter possessed significantly higher slaughter weight and carcass weight. Rabbits fed in the summer were more vulnerable to different stressors, which led to increased protein levels of HSP90, IL-1 alpha, IL-1 beta, IL-2, and concentrations of MDA, but declined GSH and SOD activities. Additionally, significant differences in gut microbial communities were observed. Compared to the winter, rabbits fed in the summer had significantly lower and higher alpha and beta diversity. Both Firmicutes and Verrucomicrobiota were the dominant phyla, and they accounted for greater proportions in the winter than in the summer. At lower microbial taxa levels, several seasonal differentially enriched microbes were identified, such as Akkermansia muciniphila, the Oscillospiraceae NK4A214 group, the Christensenellaceae R-7 group, Alistipes, and Muribaculaceae. Functional capacities linked to microbial proliferation, nutrient metabolism, and environmental adaptive responses exhibited significantly different abundances between summer and winter. Moreover, strong interactions among different indicators were presented. Based on our findings, we not only proposed several potential strategies to ameliorate the undesirable effects of seasonal changes on the productivity and health of meat rabbits but also underscored the directions for future mechanistic studies of adaptation physiology.
Keyword ：
gut microbiota gut microbiota health status health status meat rabbit meat rabbit production performance production performance seasonal variations seasonal variations semi-confined conditions semi-confined conditions
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| GB/T 7714 | Ye, Dingcheng , Ding, Xiaoning , Pang, Shuo et al. Seasonal Variations in Production Performance, Health Status, and Gut Microbiota of Meat Rabbit Reared in Semi-Confined Conditions [J]. | ANIMALS , 2024 , 14 (1) . |
| MLA | Ye, Dingcheng et al. "Seasonal Variations in Production Performance, Health Status, and Gut Microbiota of Meat Rabbit Reared in Semi-Confined Conditions" . | ANIMALS 14 . 1 (2024) . |
| APA | Ye, Dingcheng , Ding, Xiaoning , Pang, Shuo , Gan, Yating , Li, Zhechen , Gan, Qianfu et al. Seasonal Variations in Production Performance, Health Status, and Gut Microbiota of Meat Rabbit Reared in Semi-Confined Conditions . | ANIMALS , 2024 , 14 (1) . |
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肠道菌群-脑肠轴可参与新陈代谢、免疫应答和行为反应等不同生理过程，是影响生长和健康的重要因素，本研究利用多组学方法探究了湖羊肠道菌群-脑肠轴互作关系。16S-rRNA基因测序分析结果显示，厚壁菌门、拟杆菌门及克里斯腾森菌属-R7类群、肠球菌属、颤螺菌科-UCG002、普雷沃氏菌科-UCG004等菌属是湖羊肠道菌群中的主要类群。整合结肠和脑组织转录组测序分析，发现雌激素信号通路可能是连接湖羊肠道菌群-脑肠轴的关键功能途径，而真杆菌属细菌和瘤胃球菌属细菌可能是调控湖羊肠道菌群-脑肠轴的关键细菌。本研究结果能为在生产实践中通过营养干预调控肠道菌群-脑肠轴而改善湖羊性情和行为习性、提高生产性能提供基础数据。
Keyword ：
16S rRNA基因测序 16S rRNA基因测序 湖羊 湖羊 肠道菌群 肠道菌群 脑肠轴 脑肠轴 转录组测序 转录组测序
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| GB/T 7714 | 丁晓宁 , 李可瑶 , 甘雅婷 et al. 利用多组学方法探究湖羊肠道菌群-脑肠轴互作关系 [J]. | 家畜生态学报 , 2024 , 45 (09) : 7-15,90 . |
| MLA | 丁晓宁 et al. "利用多组学方法探究湖羊肠道菌群-脑肠轴互作关系" . | 家畜生态学报 45 . 09 (2024) : 7-15,90 . |
| APA | 丁晓宁 , 李可瑶 , 甘雅婷 , 庞硕 , 李哲晨 , 谢贤杰 et al. 利用多组学方法探究湖羊肠道菌群-脑肠轴互作关系 . | 家畜生态学报 , 2024 , 45 (09) , 7-15,90 . |
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Background The epididymis is a highly regionalized tubular organ possesses vectorial functions of sperm concentration, maturation, transport, and storage. The epididymis-expressed genes and proteins are characterized by regional and developmental dependent pattern. However, a systematic and comprehensive insight into the postnatal development dependent changes in gene and protein expressions of porcine epididymis is still lacking. Here, the RNA and protein of epididymis of Duroc pigs at different postnatal development stages were extracted by using commercial RNeasy Midi kit and extraction buffer (7 M Urea, 2 M thiourea, 3% CHAPS, and 1 mM PMSF) combined with sonication, respectively, which were further subjected to transcriptomic and proteomic profiling.Results Transcriptome analysis indicated that 198 and 163 differentially expressed genes (DEGs) were continuously up-regulated and down-regulated along with postnatal development stage changes, respectively. Most of the up-regulated DEGs linked to functions of endoplasmic reticulum and lysosome, while the down-regulated DEGs mainly related to molecular process of extracellular matrix. Moreover, the following key genes INSIG1, PGRMC1, NPC2, GBA, MMP2, MMP14, SFRP1, ELN, WNT-2, COL3A1, and SPARC were highlighted. A total of 49 differentially expressed proteins (DEPs) corresponding to postnatal development stages changes were uncovered by the proteome analysis. Several key proteins ACSL3 and ACADM, VDAC1 and VDAC2, and KNG1, SERPINB1, C3, and TF implicated in fatty acid metabolism, voltage-gated ion channel assembly, and apoptotic and immune processes were emphasized. In the integrative network, the key genes and proteins formed different clusters and showed strong interactions. Additionally, NPC2, COL3A1, C3, and VDAC1 are located at the hub position in each cluster.Conclusions The identified postnatal development dependent genes and proteins in the present study will pave the way for shedding light on the molecular basis of porcine epididymis functions and are useful for further studies on the specific regulation mechanisms responsible for epididymal sperm maturation.
Keyword ：
Differentially expressed genes (DEGs) Differentially expressed genes (DEGs) Differentially expressed proteins (DEPs) Differentially expressed proteins (DEPs) Duroc pigs Duroc pigs Epididymis functions Epididymis functions Molecular basis Molecular basis
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| GB/T 7714 | Fang, Shaoming , Li, Zhechen , Pang, Shuo et al. Identification of postnatal development dependent genes and proteins in porcine epididymis [J]. | BMC GENOMICS , 2023 , 24 (1) . |
| MLA | Fang, Shaoming et al. "Identification of postnatal development dependent genes and proteins in porcine epididymis" . | BMC GENOMICS 24 . 1 (2023) . |
| APA | Fang, Shaoming , Li, Zhechen , Pang, Shuo , Gan, Yating , Ding, Xiaoning , Peng, Hui . Identification of postnatal development dependent genes and proteins in porcine epididymis . | BMC GENOMICS , 2023 , 24 (1) . |
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IntroductionPollutant gas emissions in the current production system of the livestock industry have negative influences on environment as well as the health of farm staffs and animals. Although ammonia (NH3) is considered as the primary and harmful gas pollutant in the rabbit farm, less investigation has performed to determine the toxic effects of house ammonia exposure on rabbit in the commercial confined barn. MethodsIn this study, we performed multi-omics analysis on rabbits exposed to high and low concentration of house ammonia under similar environmental conditions to unravel the alterations in nasal and colonic microbiota, pulmonary and colonic gene expression, and muscular metabolic profile. Results and discussionThe results showed that house ammonia exposure notably affected microbial structure, composition, and functional capacity in both nasal and colon, which may impact on local immune responses and inflammatory processes. Transcriptome analysis indicated that genes related to cell death (MCL1, TMBIM6, HSPB1, and CD74) and immune response (CDC42, LAMTOR5, VAMP8, and CTSB) were differentially expressed in the lung, and colonic genes associated with redox state (CAT, SELENBP1, GLUD1, and ALDH1A1) were significantly up-regulated. Several key differentially abundant metabolites such as L-glutamic acid, L-glutamine, L-ornithine, oxoglutaric acid, and isocitric acid were identified in muscle metabolome, which could denote house ammonia exposure perturbed amino acids, nucleotides, and energy metabolism. In addition, the widespread and strong inter-system interplay were uncovered in the integrative correlation network, and central features were confirmed by in vitro experiments. Our findings disclose the comprehensive evidence for the deleterious effects of house ammonia exposure on rabbit and provide valuable information for understanding the underlying impairment mechanisms.
Keyword ：
house ammonia house ammonia metabolome metabolome microbiota microbiota rabbit rabbit transcriptome transcriptome
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| GB/T 7714 | Li, Keyao , Pang, Shuo , Li, Zhechen et al. House ammonia exposure causes alterations in microbiota, transcriptome, and metabolome of rabbits [J]. | FRONTIERS IN MICROBIOLOGY , 2023 , 14 . |
| MLA | Li, Keyao et al. "House ammonia exposure causes alterations in microbiota, transcriptome, and metabolome of rabbits" . | FRONTIERS IN MICROBIOLOGY 14 (2023) . |
| APA | Li, Keyao , Pang, Shuo , Li, Zhechen , Ding, Xiaoning , Gan, Yating , Gan, Qianfu et al. House ammonia exposure causes alterations in microbiota, transcriptome, and metabolome of rabbits . | FRONTIERS IN MICROBIOLOGY , 2023 , 14 . |
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Owing to the inherent heterogeneity and plasticity of fibroblasts, they are considered as the conventional biological resources for basic and clinical medical research. Thus, it is essential to generate knowledge about the establishment of fibroblast cultures and the effects of cryopreservation processes on their biological characteristics. Since the pig (Sus scrofa) possesses numerous genetic, physiological, and anatomical similarities with humans, porcine fibroblasts are naturally regarded as useful analogues of human fibroblasts. Nonetheless, less attention has been given to the alterations in viability and gene expression of cryopreserved porcine fibroblasts. In this study, we aimed to obtain fibroblasts from porcine ear skin and evaluate the effects of cryopreservation on the cell survival, proliferation, and gene expression profiles of the fibroblasts by trypan-blue-staining assay, Cell Counting kit-8 (CCK-8) assay, and RNA-sequencing analysis, respectively. Our results suggested that morphologically stable fibroblast cultures can be constructed from pig-ear skin. The post-thaw survival rate of the cryopreserved fibroblasts at 0 h and 24 h was over 90%. The proliferative activity of the cryopreserved fibroblasts was similar to that of the non-cryopreserved fibroblasts after 7 days of in vitro culture, which suggested that cryopreservation did not influence the viability. The RNA-sequencing analysis indicated that this should be attributed to the 867 differentially expressed genes (DGEs) identified, which are involved in molecular process related to cell recovery and survival after cryo-stimulation. In addition, eight important DEGs BMP2, GDF15, EREG, AREG, HBEGF, LIF, IL-6, and HOX-7 could potentially be applied to improve the efficiency of fibroblast cryopreservation, but comprehensive and systematic studies on understanding the underlying mechanisms responsible for their modulatory roles are urgently needed.
Keyword ：
cell recovery and survival cell recovery and survival cryo-stimulation cryo-stimulation fibroblast cultures fibroblast cultures RNA-sequencing RNA-sequencing
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| GB/T 7714 | Cao, Jiacheng , Xie, Yingyu , Wang, Jing et al. Evaluating the Effects of Cryopreservation on the Viability and Gene Expression of Porcine-Ear-Skin Fibroblasts [J]. | GENES , 2023 , 14 (3) . |
| MLA | Cao, Jiacheng et al. "Evaluating the Effects of Cryopreservation on the Viability and Gene Expression of Porcine-Ear-Skin Fibroblasts" . | GENES 14 . 3 (2023) . |
| APA | Cao, Jiacheng , Xie, Yingyu , Wang, Jing , Huang, Yongjie , Zhang, Xiaohan , Xiao, Tianfang et al. Evaluating the Effects of Cryopreservation on the Viability and Gene Expression of Porcine-Ear-Skin Fibroblasts . | GENES , 2023 , 14 (3) . |
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The intestinal epithelium barrier serves as a highly dynamic immunologic frontier in the defense against invading pathogenic bacteria and viruses. Hence, understanding of the complicated underlying relationship between enteric pathogens and the intestinal epithelium barrier is vital for developing strategies to improve the intestinal health of farm animals. To this end, Caco-2 cells were stimulated by 1 mu g/ml lipopolysaccharide (LPS) for 24 h and 5 mu g/ml polyinosinic-polycytidylic acid (ploy(I:C)) for 4 h to imitate bacterial and viral infection processes, respectively. The specific alterations in gene expression of Caco-2 cells after stimulation were characterized by transcriptome sequencing. Seventy differentially expressed genes (DEGs) were identified under LPS exposure, and 17 DEGs were observed under ploy(I:C) exposure. We found that most DEGs were specific, and only one common DEG SPAG7 was observed. Gene Ontology (GO) annotation analysis indicated that all DEGs identified in the different treatments were mainly derived from GO terms related to the maintenance of cellular homeostasis. Moreover, specific DEGs such as SLC39A10, MT2A, and MT1E regulated by LPS treatment, while IFIT2 and RUNX2 mediated by ploy(I:C) treatment, which are derived from immune function modulation related GO terms, were confirmed by both transcriptome sequencing and qRT-PCR. In addition, both transcriptome sequencing and qRT-PCR results verified that LPS specifically down-regulated the DEGs INHBE and ARF6, which are involved in inflammation responses related to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway including the TGF-beta signaling pathways and the Ras signaling pathway. Ploy(I:C) uniquely suppressed the DEGs GABARAP and LAMTOR3, which participated in viral replication-associated pathways including autophagy and mTOR signaling pathway.
Keyword ：
Caco-2 Caco-2 Intestinal epithelium barrier Intestinal epithelium barrier LPS LPS ploy(I:C) ploy(I:C) qRT-PCR qRT-PCR Transcriptome sequencing Transcriptome sequencing
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| GB/T 7714 | Qin, Ge , Zhao, Yuanjie , Gan, Yating et al. Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation [J]. | PEERJ , 2023 , 11 . |
| MLA | Qin, Ge et al. "Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation" . | PEERJ 11 (2023) . |
| APA | Qin, Ge , Zhao, Yuanjie , Gan, Yating , Yu, Xiaomei , Zhao, Yifan , Peng, Hui et al. Alterations in gene expressions of Caco-2 cell responses to LPS and ploy(I:C) stimulation . | PEERJ , 2023 , 11 . |
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本试验旨在研究饲粮中添加丁酸梭菌对伊拉兔盲肠菌群结构及粪便短链脂肪酸(SCFAs)含量的影响。试验选取28日龄体重相近的断奶雄性伊拉兔216只，随机分成4组，每组6个重复，每个重复9只。对照组(CG组)饲喂基础饲粮，试验组分别在基础饲粮中添加200(LC组)、400(MC组)和600 mg/kg(HC组)的丁酸梭菌。丁酸梭菌活菌数为2×108CFU/g。预试期4 d，正试期35 d。结果表明：1)各组盲肠内的优势菌门均为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)和放线菌门(Actinobacteria)。2)32和39日龄时，MC组盲肠放线菌门相对丰度显著低于CG组(P<0.05)。60和67日龄时，LC组、MC组和HC组盲肠疣微菌门相对丰度显著低于CG组(P<0.05)。3)53和67日龄时，试验组粪便乙酸、丙酸含量较CG组出现增加趋势。53日龄时，LC组粪便丁酸含量较CG组显著降低(P<0.05);67日龄时，LC组粪便异戊酸含量较CG组显著降低(P<0.05)。4)53日龄时，拟杆菌属(Bacteroides)相对丰度与乙酸含量呈显著正相关(P<0.05)；瘤胃球菌科UCG-013(Ruminococcaceae＿UCG-013)相对丰度与丙酸含量呈显著负相关(P<0.05)，与丁酸含量呈极显著负相关(P<0.01)。67日龄时，瘤胃球菌科NK4A214群(Ruminococcaceae＿NK4A214＿group)相对丰度与乙酸含量呈显著负相关(P<0.05),Ruminococcaceae＿UCG-013相对丰度与丙酸和丁酸含量呈显著负相关(P<0.05)。综上所述，饲粮中添加丁酸梭菌可改变伊拉兔盲肠菌群结构和微生物多样性，改变粪便SCFAs含量，且盲肠菌群相对丰度与粪便SCFAs含量表现出相关性。
Keyword ：
丁酸梭菌 丁酸梭菌 伊拉兔 伊拉兔 动态变化 动态变化 微生物区系 微生物区系 短链脂肪酸 短链脂肪酸
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| GB/T 7714 | 卢佳宁 , 李可瑶 , 周丽雯 et al. 饲粮中添加丁酸梭菌对伊拉兔盲肠菌群结构及粪便短链脂肪酸含量的影响 [J]. | 动物营养学报 , 2023 , 35 (05) : 3249-3265 . |
| MLA | 卢佳宁 et al. "饲粮中添加丁酸梭菌对伊拉兔盲肠菌群结构及粪便短链脂肪酸含量的影响" . | 动物营养学报 35 . 05 (2023) : 3249-3265 . |
| APA | 卢佳宁 , 李可瑶 , 周丽雯 , 叶晓星 , 李亚飞 , 刘浩宇 et al. 饲粮中添加丁酸梭菌对伊拉兔盲肠菌群结构及粪便短链脂肪酸含量的影响 . | 动物营养学报 , 2023 , 35 (05) , 3249-3265 . |
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Simple Summary In this study, multi-omics techniques were used to reveal the effects of actual temperature and humidity index (THI) on intestinal microbe, function and serum metabolism of Ira rabbits. The increase of THI had a significant effect on the intestinal microbial structure of meat rabbits. In response to environmental THI changes, intestinal microorganisms with similar functions were replaced with each other, harmful bacteria were increased, heat-sensitive factors were upregulated, and disease-related biomarkers were increased in serum. In addition, the potential biomarkers of serum metabolism could be predicted with high predictive accuracy. In future production, the rapid detection of biomarkers, which has guiding significance for scientific meat rabbit production, can be attempted to determine whether the use of THI in the small environments of meat rabbits is suitable. This study investigates the effects of different THI values on growth performance, intestinal microbes, and serum metabolism in meat rabbits. The results showed that there were significant differences in THI in different location regions of the rabbit house. The high-THI group (HG) could significantly reduce average daily gain and average daily feed intake in Ira rabbits (p < 0.05). The low-THI group (LG) significantly increased the relative abundance of Blautia (p < 0.05). The HG significantly increased the relative abundance of Lachnospiraceae NK4A136 group and reduced bacterial community interaction (p < 0.05). The cytokine-cytokine receptor interactions, nuclear factor kappa B signaling pathway, and toll-like receptor signaling pathway in each rabbit's gut were activated when the THI was 26.14 (p < 0.05). Metabolic pathways such as the phenylalanine, tyrosine, and tryptophan biosynthesis and phenylalanine metabolisms were activated when the THI was 27.25 (p < 0.05). Meanwhile, the TRPV3 and NGF genes that were associated with heat sensitivity were significantly upregulated (p < 0.05). In addition, five metabolites were found to be able to predict THI levels in the environment with an accuracy of 91.7%. In summary, a THI of 26.14 is more suitable for the growth of meat rabbits than a THI of 27.25, providing a reference for the efficient feeding of meat rabbits.
Keyword ：
colon microbiota colon microbiota growth performance growth performance Ira rabbits Ira rabbits serum metabolites serum metabolites temperature and humidity index temperature and humidity index
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| GB/T 7714 | Li, Keyao , Abdelsattar, Mahmoud M. , Gu, Mingming et al. The Effects of Temperature and Humidity Index on Growth Performance, Colon Microbiota, and Serum Metabolome of Ira Rabbits [J]. | ANIMALS , 2023 , 13 (12) . |
| MLA | Li, Keyao et al. "The Effects of Temperature and Humidity Index on Growth Performance, Colon Microbiota, and Serum Metabolome of Ira Rabbits" . | ANIMALS 13 . 12 (2023) . |
| APA | Li, Keyao , Abdelsattar, Mahmoud M. , Gu, Mingming , Zhao, Wei , Liu, Haoyu , Li, Yafei et al. The Effects of Temperature and Humidity Index on Growth Performance, Colon Microbiota, and Serum Metabolome of Ira Rabbits . | ANIMALS , 2023 , 13 (12) . |
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Liquid preservation of boar sperm is crucial for artificial insemination application in pig production. However, time-dependent oxidative damage to sperm is one of the major challenges during the liquid preservation period. Caffeic acid phenethyl ester (CAPE) possesses excellent antioxidant properties and has potential therapeutic use in reproductive organ injury linked to oxidative stress. Adenosine monophosphate (AMP)-activated protein kinase (AMPK) involves in modulating the cellular redox state and exerts a beneficial effect on sperm preservation. In the present study, we firstly assessed different concentrations of CAPE that affect sperm quality during liquid storage to determine the appropriate addition. To further investigate whether CAPE exerts protective effects on boar sperm through modulation of AMPK activity, sperm quality parameters, antioxidant capacity, and marker protein expressions were evaluated under co-incubation with H2O2. The results showed that sperm treated with 210 mu mol/L CAPE exhibited the highest motion parameters (total motility and progressive motility) and best functional integrity (mitochondrial activity, plasma membrane integrity, and acrosomal integrity). Even in the presence of H2O2, the addition of 210 mu mol/L CAPE not only significantly improved sperm quality parameters, but also elevated CAT, SOD, and GSH-Px activities to enhance sperm antioxidant capacity. In addition, we found that CAPE could affect the protein activities of AMPK, phospho-AMPK alpha (p-AMPK), SOD, and Caspase-3 regardless of whether H2O2 is present or not. Our findings suggested that CAPE has potential application in liquid preservation of boar sperm and preliminary indicated that CAPE-induced improvement of sperm quality and antioxidant capacity should be mediated through conservation of AMPK activity. Further studies are required to illustrate the specific mechanism by which CAPE attenuates oxidative stress-mediated damages dependent on AMPK activity.
Keyword ：
AMPK AMPK antioxidant capacity antioxidant capacity boar sperm boar sperm CAPE CAPE liquid preservation liquid preservation sperm quality sperm quality
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| GB/T 7714 | Lan, Qun , Xue, Li'e , Cao, Jiacheng et al. Caffeic Acid Phenethyl Ester (CAPE) Improves Boar Sperm Quality and Antioxidant Capacity in Liquid Preservation (17°C) Linked to AMPK Activity Maintenance [J]. | FRONTIERS IN VETERINARY SCIENCE , 2022 , 9 . |
| MLA | Lan, Qun et al. "Caffeic Acid Phenethyl Ester (CAPE) Improves Boar Sperm Quality and Antioxidant Capacity in Liquid Preservation (17°C) Linked to AMPK Activity Maintenance" . | FRONTIERS IN VETERINARY SCIENCE 9 (2022) . |
| APA | Lan, Qun , Xue, Li'e , Cao, Jiacheng , Xie, Yingyu , Xiao, Tianfang , Fang, Shaoming . Caffeic Acid Phenethyl Ester (CAPE) Improves Boar Sperm Quality and Antioxidant Capacity in Liquid Preservation (17°C) Linked to AMPK Activity Maintenance . | FRONTIERS IN VETERINARY SCIENCE , 2022 , 9 . |
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Clostridium butyricum (C. butyricum) can provide many benefits for animals' growth performance and gut health. In this study, we investigated the effects of C. butyricum on the growth performance, cecal microbiota, and plasma metabolome in Ira rabbits. A total of 216 Ira rabbits at 32 days of age were randomly assigned to four treatments supplemented with basal diets containing 0 (CG), 200 (LC), 400 (MC), and 600 mg/kg (HC) C. butyricum for 35 days, respectively. In comparison with the CG group, C. butyricum supplementation significantly improved the average daily gain (ADG) and feed conversion rate (FCR) at 53 and 67 days of age (P < 0.05) and digestibilities of crude protein (CP) and crude fiber (CF) at 67 days of age (P < 0.05). The cellulase activity in the HC group was higher respectively by 50.14 and 90.13% at 53 and 67 days of age, than those in the CG groups (P < 0.05). Moreover, at 67 days of age, the diet supplemented with C. butyricum significantly increased the relative abundance of Verrucomicrobia at the phylum level (P < 0.05). Meanwhile, the concentrations of different metabolites, such as amino acids and purine, were significantly altered by C. butyricum (P < 0.05). In addition, 10 different genera were highly correlated with 52 different metabolites at 53-day-old and 6 different genera were highly correlated with 18 different metabolites at 67-day-old Ira rabbits. These findings indicated that the C. butyricum supplementation could significantly improve the growth performance by modifying the cecal microbiota structure and plasma metabolome of weaned Ira rabbits.
Keyword ：
butyricum butyricum C C growth performance growth performance gut microbiota gut microbiota Ira rabbits Ira rabbits plasma metabolites plasma metabolites
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| GB/T 7714 | Ye, Xiao Xing , Li, Ke Yao , Li, Ya Fei et al. The effects of Clostridium butyricum on Ira rabbit growth performance, cecal microbiota and plasma metabolome [J]. | FRONTIERS IN MICROBIOLOGY , 2022 , 13 . |
| MLA | Ye, Xiao Xing et al. "The effects of Clostridium butyricum on Ira rabbit growth performance, cecal microbiota and plasma metabolome" . | FRONTIERS IN MICROBIOLOGY 13 (2022) . |
| APA | Ye, Xiao Xing , Li, Ke Yao , Li, Ya Fei , Lu, Jia Ning , Guo, Ping Ting , Liu, Hao Yu et al. The effects of Clostridium butyricum on Ira rabbit growth performance, cecal microbiota and plasma metabolome . | FRONTIERS IN MICROBIOLOGY , 2022 , 13 . |
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