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学者姓名：王路路
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Abstract ：
菠萝（Ananas comosus）是一种营养丰富、风味独特的水果，为全球贸易量第二大的热带水果，在我国热带农业经济中占据重要地位，广西是我国菠萝的主产区之一。本研究旨在系统评估广西菠萝产业发展现状，识别制约产业升级的关键瓶颈，并提出科学对策，为区域农业供给侧结构性改革与乡村振兴战略提供重要依据。基于2019―2023年广西农业农村厅统计数据，结合菠萝主产区实地调研与CNKI数据库文献分析，采用描述性统计、产业竞争力分析及典型案例研究法，从产业地位和历史沿革、产业布局及品种结构、生产效率与经济效益、生产技术与管理水平、市场销售与品牌建设等维度解析菠萝产业动态。结果表明，2019―2023年，广西菠萝种植面积年均减少了8.9%，但单产提高了79.49%，每公顷产值增长了55.24%，呈现单产水平、单位面积附加值大幅度提高，产业效益显著提升的特征。品种更新比例达40%，台农系列及金菠萝等新品种推广成效显著，标准化生产技术推动每公顷产量突破42 000 kg。然而，品种单一化（巴厘品种占比60%）、寒害与凋萎病频发（年均受灾面积占比31.7%）、加工率低（11.5%）等问题仍制约产业可持续发展。建议通过加强种质创新与优化产业布局，研发新品种配套栽培技术体系并进行示范推广，构建智能化绿色病虫害防控体系，完善采后处理体系、延伸加工产业链、提升产业组织化与市场推广效能等措施，实现广西菠萝产业从“面减质升”向“质效双增”的跨越式发展。本研究的成果可为热带水果产业高质量发展提供参考。
Keyword ：
产业链协同 产业链协同 产期调控 产期调控 广西 广西 菠萝 菠萝 避寒栽培 避寒栽培
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| GB/T 7714 | 邓彪 , 刘开创 , 柴改凤 et al. 广西菠萝产业现状分析及发展对策 [J]. | 农业研究与应用 , 2025 , 38 (03) : 226-234 . |
| MLA | 邓彪 et al. "广西菠萝产业现状分析及发展对策" . | 农业研究与应用 38 . 03 (2025) : 226-234 . |
| APA | 邓彪 , 刘开创 , 柴改凤 , 郑平 , 王路路 , 陶慧 et al. 广西菠萝产业现状分析及发展对策 . | 农业研究与应用 , 2025 , 38 (03) , 226-234 . |
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Passion fruit (Passiflora edulis), mainly distributed in tropical and subtropical regions, is popular for its unique flavor and health benefits. The actin cytoskeleton plays a crucial role in plant growth and development, and villin is a key regulator of actin dynamics. However, the mechanism underlying the actin filament regulation of reproductive development in passion fruit remains poorly understood. Here, we characterized a villin isovariant in passion fruit, Passiflora edulis VLN4 (PeVLN4), highly and preferentially expressed in pollen. Subcellular localization analysis showed that PeVLN4 decorated distinct filamentous structures in pollen tubes. We next introduced PeVLN4 into Arabidopsis villin mutants to explore its functions on the growing pollen tubes. PeVLN4 rescued defects in the elongation of villin mutant pollen tubes. Pollen tubes expressing PeVLN4 were revealed to be less sensitive to latrunculin B, and PeVLN4 partially rescued defects in the actin filament organization of villin mutant pollen tubes. Additionally, biochemical assays revealed that PeVLN4 bundles actin filaments in vitro. Thus, PeVLN4 is an important regulator of F-actin stability and is required for normal pollen tube growth in passion fruit. This study provides a new insight into the function of the actin regulator villin involved in the reproduction development of passion fruit.
Keyword ：
actin filament actin filament Passiflora edulis Passiflora edulis reproduction development reproduction development VLN gene VLN gene
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| GB/T 7714 | Yang, Hanbing , Wei, Xiuqing , Wang, Lifeng et al. Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
| MLA | Yang, Hanbing et al. "Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 5 (2025) . |
| APA | Yang, Hanbing , Wei, Xiuqing , Wang, Lifeng , Zheng, Ping , Li, Junzhang , Zou, Yutong et al. Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
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BackgroundPineapple (Ananas comosus L.), an economically significant tropical fruit crop, is highly susceptible to low-temperature during cultivation. The abscisic acid (ABA) and stress-inducible HVA22 gene family is known to play important roles in growth regulation and abiotic stress response, but its functions in pineapple remain unclear.ResultsWe identified 11 HVA22 genes (AcHVA22A - AcHVA22K) in the pineapple genome and grouped them into four phylogenetic clades. Expression analysis showed that most AcHVA22 genes displayed tissue- or developmental stage-specific expression patterns, suggesting their diverse functions in pineapple growth and development. Subcellular localization analyses revealed diverse localizations of AcHVA22 proteins, including plasma membrane, cytoplasm, and nucleus. And regulatory predictions indicated control by multiple cis-elements, transcription factors, and miRNAs, which may contribute to their functional diversification. Most AcHVA22 genes responded consistently to ABA, GA, and drought treatments, but their responses to high and low-temperatures varied. Notably, AcHVA22C/D/E/G/I/K were up-regulated under both short- and long-term cold treatments in two different pineapple varieties, highlighting their potential key roles in cold stress tolerance.ConclusionsThis study provided the first genome-wide characterization of the HVA22 gene family in pineapple. The identification of candidate genes involved in cold stress response offers new insights into HVA22 functions in tropical fruits and provides valuable resources for improving cold resistance in pineapple breeding.
Keyword ：
Cold response Cold response Expression profiling Expression profiling HVA22 genes HVA22 genes Pineapple Pineapple Tissue-specific Tissue-specific
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| GB/T 7714 | Hou, Zhimin , Cai, Xinkai , Wu, Jiahao et al. Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response [J]. | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
| MLA | Hou, Zhimin et al. "Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response" . | BMC PLANT BIOLOGY 25 . 1 (2025) . |
| APA | Hou, Zhimin , Cai, Xinkai , Wu, Jiahao , Lu, Lin , Liu, Chaojia , Zhang, Yangmei et al. Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response . | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
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Microspore culture is an efficient and widely used technology in plant breeding for the production of haploid and (DH) plants that are widely used in fast-tracking crop improvement. This technique consists of steps of culturing microspores or immature pollen grains and inducing haploid embryos that generate viable plants that are doubled in chromosomes resulting in homozygosity across all loci. The DH lines are highly effective in shortening the genetic improvement timeline of many crops and are frequently used in hybrid development and analysis of complex traits. Microspore culture also provides new resources for breeding through spontaneous mutations and somaclonal variations. These variations can be used to create new characters, which are desirable for breeding crops' disease resistance and stress tolerance. Also, the technique can be used for the preservation of genetic resources and as an excellent tool for genome manipulation through efficient and accurate introduction of desirable traits into the target crop plants. However, the use of microspore culture is hampered in many crops by species-specific success rates and genetic instability during in vitro culture. Current research aims to optimize protocols across a broader range of crops and integrate microspore culture with emerging technologies such as high-throughput phenotyping and bioinformatics to improve its efficiency and expand its use.
Keyword ：
Breeding Breeding Crop improvement Crop improvement Double haploids Double haploids Genetic diversity Genetic diversity Microspore culture Microspore culture
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| GB/T 7714 | Arabzai, Mohammad Gul , Huang, Dongping , Mohammadi, Nazir Khan et al. Techniques and advantages of microspore culture for crop improvement [J]. | PLANT GROWTH REGULATION , 2025 , 105 (4) : 903-918 . |
| MLA | Arabzai, Mohammad Gul et al. "Techniques and advantages of microspore culture for crop improvement" . | PLANT GROWTH REGULATION 105 . 4 (2025) : 903-918 . |
| APA | Arabzai, Mohammad Gul , Huang, Dongping , Mohammadi, Nazir Khan , Gao, Jingai , Wang, Xiaomei , Zheng, Ping et al. Techniques and advantages of microspore culture for crop improvement . | PLANT GROWTH REGULATION , 2025 , 105 (4) , 903-918 . |
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Environmental constraints such as drought and salt stress severely limit crop production worldwide. The WRKY TFs are very important in regulating plant growth and stress responses. Pineapple (Ananas comosus) is widely grown due to its unique flavor, high vitamins, and dietary fiber; however, the functional characterization of WRKY TFs in pineapple remains largely unexplored. In our study, we amplified and explored the molecular function of pineapple AcWRKY27, including its conserved domain, protein localization, transcriptional activity, and expression profiles in different tissues and stress treatments. Overexpression of AcWRKY27 in both rice and Arabidopsis thaliana (A. thaliana) resulted in growth inhibition, a decrease in primary root elongation, and a reduction in fresh weight under salt, drought, and ABA stress. RNA-Seq analysis and quantitative PCR (RT-qPCR) revealed that AcWRKY27 overexpression resulted in decreased expression levels of stress-responsive and ABA signaling pathway genes. These findings provide new perspectives on pineapple WRKY TFs and lay a foundation for improving pineapple stress tolerance through molecular breeding in the future.
Keyword ：
ABA ABA AcWRKY AcWRKY salt and drought stress salt and drought stress transcription factor transcription factor
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| GB/T 7714 | Chai, Gaifeng , Huang, Dongping , Gao, Jingai et al. Ectopic Expression of Pineapple AcWRKY27 Increases Sensitivity to Abiotic Stress in Rice and Arabidopsis [J]. | PHYSIOLOGIA PLANTARUM , 2025 , 177 (6) . |
| MLA | Chai, Gaifeng et al. "Ectopic Expression of Pineapple AcWRKY27 Increases Sensitivity to Abiotic Stress in Rice and Arabidopsis" . | PHYSIOLOGIA PLANTARUM 177 . 6 (2025) . |
| APA | Chai, Gaifeng , Huang, Dongping , Gao, Jingai , Wu, Ting , Xu, Lixin , Arabzai, Mohammad Gul et al. Ectopic Expression of Pineapple AcWRKY27 Increases Sensitivity to Abiotic Stress in Rice and Arabidopsis . | PHYSIOLOGIA PLANTARUM , 2025 , 177 (6) . |
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The HKT protein family plays a vital role in plant responses to salt stress by mediating sodium (Na+) and potassium (K+) transport and maintaining Na+-K+ balance. Ipomoea pes-caprae (IPC), a pantropical creeping plant distributed along coastal regions in tropical and subtropical zones, exhibits exceptional salt tolerance. Understanding its salt tolerance mechanisms provides valuable insights for developing salt-tolerant crops and identifying candidate genes for genetic engineering. In this study, we identified two HKT genes, IpcHKT1;1 and IpcHKT1;2, in IPC. Phylogenetic analysis with HKT genes from other Ipomoea species revealed that all analyzed species contain two HKT genes located adjacently on the same chromosome. Comparative analysis of conserved motifs and intron-exon structures indicated that, despite their close evolutionary relationship, the HKT genes in IPC may exhibit functional divergence. Promoter analysis showed that their regulatory regions are enriched with cis-elements associated with responses to biotic and abiotic stresses, hormonal signaling, and growth, highlighting functional diversity within the HKT family. Subcellular localization experiments demonstrated that IpcHKT1;1 and IpcHKT1;2 are ion transporters localized to the plasma membrane. Heterologous expression in yeast confirmed their role in Na+/K+ symporter. Furthermore, RT-qPCR analysis revealed distinct expression patterns under salt stress: IpcHKT1;2 was significantly upregulated in roots, while IpcHKT1;1 expression was transitionally downregulated at 400 mM NaCl treatment. Prolonged high expression of IpcHKT1;2 in roots suggests its critical role in sustained salt stress tolerance. These findings provide new insights into the molecular mechanisms of salt tolerance in IPC. The identification of IpcHKT1;1 and IpcHKT1;2 as key players in salt stress responses offers promising genetic resources for enhancing crop resilience to soil salinity, addressing challenges associated with global salinization.
Keyword ：
halophytes halophytes HKT HKT Ipomoea pes-caprae Ipomoea pes-caprae salt stress salt stress sodium-potassium transport sodium-potassium transport
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| GB/T 7714 | Guo, Zhonghua , Sun, Jin , Chen, Xingguang et al. Comparative analysis of HKT genes in Ipomoea pes-caprae unveils conserved Na+/K+ symporter functions within the gene family [J]. | FRONTIERS IN PLANT SCIENCE , 2025 , 16 . |
| MLA | Guo, Zhonghua et al. "Comparative analysis of HKT genes in Ipomoea pes-caprae unveils conserved Na+/K+ symporter functions within the gene family" . | FRONTIERS IN PLANT SCIENCE 16 (2025) . |
| APA | Guo, Zhonghua , Sun, Jin , Chen, Xingguang , Li, Hui , Liang, Sisi , Liu, Fengying et al. Comparative analysis of HKT genes in Ipomoea pes-caprae unveils conserved Na+/K+ symporter functions within the gene family . | FRONTIERS IN PLANT SCIENCE , 2025 , 16 . |
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The cell cycle is a fundamental process of plant growth, development, and reproduction, in which cyclin-dependent kinases (CDKs) and cyclins (CYCs) play central roles in regulating the progression through various stages. These proteins are coordinated with multiple interacting partners to ensure the accurate execution of essential biological events such as DNA replication, chromosome segregation, and cell division. Marchantia polymorpha, one of the earliest diverging land plant species, has emerged as a key model for exploring fundamental mechanisms in plant biology and evolution. However, compared with other model plants, such as Arabidopsis thaliana and Oryza sativa, the core cell cycle genes in M. polymorpha remain relatively uncharacterized. In this study, we identified 31 core cell cycle genes in M. polymorpha through genome-wide analysis, including 13 CDKs, 8 CYCs, 5 E2F/DPs, 1 ICK, 1 RB, 1 CKS, and 2 Wee1 genes. We further analyzed their physicochemical properties, gene structures, and conserved domains, along with evolutionary pressures assessed via Ka/Ks and 4DTv analyses. Comparative genomic analysis revealed patterns of gene contraction and expansion. Additionally, we predicted cis-acting regulatory elements and performed differential expression analysis under various stress conditions to explore their potential functions and expression profiles. Finally, a protein-protein interaction (PPI) network was constructed, and key genes were experimentally validated. These findings provide valuable insights into the core cell cycle gene family in M. polymorpha, contributing to an enhanced understanding of cell cycle regulation and its evolutionary significance in plants.
Keyword ：
core cell cycle genes core cell cycle genes evolutionary analysis evolutionary analysis gene redundancy gene redundancy genome analysis genome analysis Marchantia polymorpha Marchantia polymorpha
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| GB/T 7714 | Chen, Xingguang , Feng, Haoran , Liu, Mengjuan et al. Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution [J]. | PHYSIOLOGIA PLANTARUM , 2025 , 177 (5) . |
| MLA | Chen, Xingguang et al. "Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution" . | PHYSIOLOGIA PLANTARUM 177 . 5 (2025) . |
| APA | Chen, Xingguang , Feng, Haoran , Liu, Mengjuan , Cai, Jiahao , Sarwar, Rabia , Li, Xueli et al. Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution . | PHYSIOLOGIA PLANTARUM , 2025 , 177 (5) . |
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Nitrogen (N) is crucial for plant growth and stress resistance and is primarily absorbed and transported by nitrate transporters (NRT). Suaeda glauca, known for its strong salt-alkali stress resistance, and SgNRT genes have rarely been reported. This study aims to identify and analyze the SgNRT gene family to understand its composition, evolutionary patterns, and roles in salt stress responses. We identified 212 SgNRTs, which were categorized into three branches, with SgNRT1/SgNPF and SgNRT2 as the major families. Structural analysis, conserved domains, chromosomal localization, and collinearity were also examined. Spatiotemporal expression characteristics of SgNRT genes were analyzed, revealing specific expression across 13 organs or tissues and dynamic responses to salt treatment over 48 h. Notably, SgNRT1.185, SgNRT2.25, and SgNRT2.2 exhibited rapid salt induction in leaves (activated within 0.5 h, peaking at 2 h), with SgNRT1.185 showing relatively high upregulation. SgNRT1.185 and SgNRT2.35 were induced by high salt concentrations (200 mM) in both roots and leaves. SgNRT2.35 exhibited higher basal and stress-induced levels than the other genes. Bioinformatics analysis suggests spatially specific expression of SgNRT genes, potentially involved in nitrogen absorption and transport across various developmental stages and organs/tissues of Suaeda glauca. These findings offer a theoretical basis for understanding the adaptive strategies of Suaeda glauca under saline-alkali stress and provide insights into the functional evolution of plant NRT genes, aiding in the development of stress-resistant crops.
Keyword ：
bioinformatics bioinformatics gene expression analysis gene expression analysis NRT gene family NRT gene family soil salinization soil salinization Suaeda glauca Suaeda glauca
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| GB/T 7714 | Ou, Zitong , Sun, Jin , Li, Xueli et al. Genome-Wide Identification, Characterization, and Expression Analysis of NRT Gene Family in Suaeda glauca [J]. | BIOLOGY-BASEL , 2025 , 14 (8) . |
| MLA | Ou, Zitong et al. "Genome-Wide Identification, Characterization, and Expression Analysis of NRT Gene Family in Suaeda glauca" . | BIOLOGY-BASEL 14 . 8 (2025) . |
| APA | Ou, Zitong , Sun, Jin , Li, Xueli , Feng, Haoran , Chen, Xingguang , Liang, Sisi et al. Genome-Wide Identification, Characterization, and Expression Analysis of NRT Gene Family in Suaeda glauca . | BIOLOGY-BASEL , 2025 , 14 (8) . |
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本发明公开了菠萝转录因子AcWRKY38基因的克隆方法、表达载体构建及其在调控植物冷胁迫中的应用，首先提取菠萝叶片的RNA，反转录获得cDNA；再以菠萝cDNA为模版，根据目的基因的序列设计特异性引物，通过特异性引物AcWRKY38F，AcWRKY38R进行PCR扩增获得目的片段。AcWRKY38基因的目的片段连接到pENTR载体上并进行热激转化，连接成功进行PCR鉴定，测序无误后，提取质粒。然后将已连接上GATEWAY入门载体pENTR的目的载体与中间载体pGWB505进行LR重组反应。构建成功后，利用农杆菌介导的遗传转化技术将目的基因转入菠萝愈伤组织，通过潮霉素筛选，获得菠萝过表达阳性植株。将转基因菠萝植株通过冷胁迫处理，通过表型观察发现，过表达AcWRKY38转基因菠萝植株增强了菠萝耐冷和抗逆性，有利于培育高产、优质、高抗性的优良菠萝品种。
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| GB/T 7714 | 王路路 , 柴改凤 , 秦源 et al. 菠萝转录因子AcWRKY38基因的克隆、表达载体构建及其在菠萝寒害中的应用 : CN202311775305.5[P]. | 2023-12-22 . |
| MLA | 王路路 et al. "菠萝转录因子AcWRKY38基因的克隆、表达载体构建及其在菠萝寒害中的应用" : CN202311775305.5. | 2023-12-22 . |
| APA | 王路路 , 柴改凤 , 秦源 , 黄东平 , 王小媚 , 程焱 et al. 菠萝转录因子AcWRKY38基因的克隆、表达载体构建及其在菠萝寒害中的应用 : CN202311775305.5. | 2023-12-22 . |
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本发明公开一种阻断菠萝成熟果实水心病增加的方法，属于果树栽培技术领域。本发明方法包括：4～9月菠萝果实采收期，根据天气变化情况抽样采收达到不同成熟度的果实，先将果园划分多个区域，在每个区域随机抽样选取不同成熟度的果实，判断其出现水心病发生情况，进而确定该区域菠萝果实水心病的发生率，对于达到采收标准六成或七成熟的菠萝果实水心病发生率在20％以上的区域优先采收，将该区域的全部果实的果柄直接截断，可以有效的阻断了果实水心病的发生率，之后再对果园中余下的区域逐步分批采收。该方法解决了大面积菠萝生产果园短期内采收不及时出现的果实水心病发生加重的问题，既简单又安全高效，对于菠萝产业的可持续发展具有重要意义。
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| GB/T 7714 | 王小媚 , 秦源 , 邓彪 et al. 一种阻断菠萝成熟果实水心病增加的方法 : CN202411316928.0[P]. | 2024-09-20 . |
| MLA | 王小媚 et al. "一种阻断菠萝成熟果实水心病增加的方法" : CN202411316928.0. | 2024-09-20 . |
| APA | 王小媚 , 秦源 , 邓彪 , 王路路 , 郑平 , 柴改凤 et al. 一种阻断菠萝成熟果实水心病增加的方法 : CN202411316928.0. | 2024-09-20 . |
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