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学者姓名：党谢
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© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.Many studies from different model organisms have demonstrated that microtubules are essential for various cellular processes, including cell division, cell morphogenesis, and intracellular trafficking. In interphase plant cells, oriented cortical microtubule arrays are highly characteristic in cells that display various morphologies, such as elongated hypocotyl cells and root cells, jigsaw-puzzled leaf pavement cells, and petal epidermal conical cells. Conical cells represent a specialized epidermal cell type found in the petal epidermis of many flowering plants. It has been suggested that in the model plant Arabidopsis thaliana, the petal adaxial epidermal cells develop from a roughly hemispherical morphology to a conical shape, correlating with the reorientation of cortical microtubules from random to well-ordered circumferential arrays. This chapter presents an overview of the methods available to visualize the microtubule cytoskeleton in living conical cells via confocal microscopy.
Keyword ：
Cell biology Cell biology Confocal microscope Confocal microscope Conical cells Conical cells Fluorescent-tagged reporters Fluorescent-tagged reporters Live-cell imaging Live-cell imaging Microtubule Microtubule Petal Petal Plant Plant
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| GB/T 7714 | Dang, Xie , Zhu, Lilan , Ren, Huibo et al. Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells. [J]. | Methods in Molecular Biology , 2023 , 2604 : 317-325 . |
| MLA | Dang, Xie et al. "Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells." . | Methods in Molecular Biology 2604 (2023) : 317-325 . |
| APA | Dang, Xie , Zhu, Lilan , Ren, Huibo , Lin, Deshu . Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells. . | Methods in Molecular Biology , 2023 , 2604 , 317-325 . |
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Sclerotinia sclerotiorum is a devastating pathogen that infects a broad range of host plants. The mechanism underlying plant defence against fungal invasion is still not well characterized. Here, we report that ANGUSTIFOLIA (AN), a CtBP family member, plays a role in the defence against S. sclerotiorum attack. Arabidopsis an mutants exhibited stronger resistance to S. sclerotiorum at the early stage of infection than wild-type plants. Accordingly, an mutants exhibited stronger activation of pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) responses, including mitogen-activated protein kinase activation, reactive oxygen species accumulation, callose deposition, and the expression of PTI-responsive genes, upon treatment with PAMPs/microbe-associated molecular patterns. Moreover, Arabidopsis lines overexpressing AN were more susceptible to S. sclerotiorum and showed defective PTI responses. Our luminometry, bimolecular fluorescence complementation, coimmunoprecipitation, and in vitro pull-down assays indicate that AN interacts with allene oxide cyclases (AOC), essential enzymes involved in jasmonic acid (JA) biosynthesis, negatively regulating JA biosynthesis in response to S. sclerotiorum infection. This work reveals AN is a negative regulator of the AOC-mediated JA signalling pathway and PTI activation.
Keyword ：
ANGUSTIFOLIA gene ANGUSTIFOLIA gene Arabidopsis thaliana Arabidopsis thaliana JA signalling pathway JA signalling pathway PAMP-triggered immunity PAMP-triggered immunity Sclerotinia sclerotiorum Sclerotinia sclerotiorum
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| GB/T 7714 | Gao, Xiuqin , Dang, Xie , Yan, Fengting et al. ANGUSTIFOLIA negatively regulates resistance to Sclerotinia sclerotiorum via modulation of PTI and JA signalling pathways in Arabidopsis thaliana [J]. | MOLECULAR PLANT PATHOLOGY , 2022 , 23 (8) : 1091-1106 . |
| MLA | Gao, Xiuqin et al. "ANGUSTIFOLIA negatively regulates resistance to Sclerotinia sclerotiorum via modulation of PTI and JA signalling pathways in Arabidopsis thaliana" . | MOLECULAR PLANT PATHOLOGY 23 . 8 (2022) : 1091-1106 . |
| APA | Gao, Xiuqin , Dang, Xie , Yan, Fengting , Li, Yuhua , Xu, Jing , Tian, Shifu et al. ANGUSTIFOLIA negatively regulates resistance to Sclerotinia sclerotiorum via modulation of PTI and JA signalling pathways in Arabidopsis thaliana . | MOLECULAR PLANT PATHOLOGY , 2022 , 23 (8) , 1091-1106 . |
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During growth and morphogenesis, plant cells respond to mechanical stresses resulting from spatiotemporal changes in the cell wall that bear high internal turgor pressure. Microtubule (MT) arrays are reorganized to align in the direction of maximal tensile stress, presumably reinforcing the local cell wall by guiding the synthesis of cellulose. However, how mechanical forces regulate MT reorganization remains largely unknown. Here, we demonstrate that mechanical signaling that is based on the Catharanthus roseus RLK1-like kinase (CrRLK1L) subfamily receptor kinase FERONIA (FER) regulates the reorganization of cortical MT in cotyledon epidermal pavement cells (PCs) in Arabidopsis. Recessive mutations in FER compromised MT responses to mechanical perturbations, such as single-cell ablation, compression, and isoxaben treatment, in these PCs. These perturbations promoted the activation of ROP6 guanosine triphosphatase (GTPase) that acts directly downstream of FER. Furthermore, defects in the ROP6 signaling pathway negated the reorganization of cortical MTs induced by these stresses. Finally, reduction in highly demethylesterified pectin, which binds the extracellular malectin domains of FER and is required for FER-mediated ROP6 activation, also impacted mechanical induction of cortical MT reorganization. Taken together, our results suggest that the FER-pectin complex senses and/or transduces mechanical forces to regulate MT organization through activating the ROP6 signaling pathway in Arabidopsis.
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| GB/T 7714 | Tang, Wenxin , Lin, Wenwei , Zhou, Xiang et al. Mechano-transduction via the pectin-FERONIA complex activates ROP6 GTPase signaling in Arabidopsis pavement cell morphogenesis [J]. | CURRENT BIOLOGY , 2022 , 32 (3) : 508-, . |
| MLA | Tang, Wenxin et al. "Mechano-transduction via the pectin-FERONIA complex activates ROP6 GTPase signaling in Arabidopsis pavement cell morphogenesis" . | CURRENT BIOLOGY 32 . 3 (2022) : 508-, . |
| APA | Tang, Wenxin , Lin, Wenwei , Zhou, Xiang , Guo, Jingzhe , Dang, Xie , Li, Binqi et al. Mechano-transduction via the pectin-FERONIA complex activates ROP6 GTPase signaling in Arabidopsis pavement cell morphogenesis . | CURRENT BIOLOGY , 2022 , 32 (3) , 508-, . |
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The organization of the microtubule cytoskeleton is critical for cell and organ morphogenesis. The evolutionarily conserved microtubule-severing enzyme KATANIN plays critical roles in microtubule organization in the plant and animal kingdoms. We previously used conical cell of Arabidopsis thaliana petals as a model system to investigate cortical microtubule organization and cell morphogenesis and determined that KATANIN promotes the formation of circumferential cortical microtubule arrays in conical cells. Here, we demonstrate that the conserved protein phosphatase PP2A interacts with and dephosphorylates KATANIN to promote the formation of circumferential cortical microtubule arrays in conical cells. KATANIN undergoes cycles of phosphorylation and dephosphorylation. Using co-immunoprecipitation coupled with mass spectrometry, we identified PP2A subunits as KATANIN-interacting proteins. Further biochemical studies showed that PP2A interacts with and dephosphorylates KATANIN to stabilize its cellular abundance. Similar to the katanin mutant, mutants for genes encoding PP2A subunits showed disordered cortical microtubule arrays and defective conical cell shape. Taken together, these findings identify PP2A as a regulator of conical cell shape and suggest that PP2A mediates KATANIN phospho-regulation during plant cell morphogenesis.
Keyword ：
conical cells conical cells cortical microtubules cortical microtubules KATANIN KATANIN morphogenesis morphogenesis phospho-regulation phospho-regulation protein phosphatase PP2A protein phosphatase PP2A
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| GB/T 7714 | Ren, Huibo , Rao, Jinqiu , Tang, Min et al. PP2A interacts with KATANIN to promote microtubule organization and conical cell morphogenesis [J]. | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2022 , 64 (8) : 1514-1530 . |
| MLA | Ren, Huibo et al. "PP2A interacts with KATANIN to promote microtubule organization and conical cell morphogenesis" . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY 64 . 8 (2022) : 1514-1530 . |
| APA | Ren, Huibo , Rao, Jinqiu , Tang, Min , Li, Yaxing , Dang, Xie , Lin, Deshu . PP2A interacts with KATANIN to promote microtubule organization and conical cell morphogenesis . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2022 , 64 (8) , 1514-1530 . |
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Plant cells continuously experience mechanical stress resulting from the cell wall that bears internal turgor pressure. Cortical microtubules align with the predicted maximal tensile stress direction to guide cellulose biosynthesis and therefore results in cell wall reinforcement. We have previously identified Increased Petal Growth Anisotropy (IPGA1) as a putative microtubule-associated protein in Arabidopsis, but the function of IPGA1 remains unclear. Here, using the Arabidopsis cotyledon pavement cell as a model, we demonstrated that IPGA1 forms protein granules and interacts with ANGUSTIFOLIA (AN) to cooperatively regulate microtubule organisation in response to stress. Application of mechanical perturbations, such as cell ablation, led to microtubule reorganisation into aligned arrays in wild-type cells. This microtubule response to stress was enhanced in the IPGA1 loss-of-function mutant. Mechanical perturbations promoted the formation of IPGA1 granules on microtubules. We further showed that IPGA1 physically interacted with AN both in vitro and on microtubules. The ipga1 mutant alleles exhibited reduced interdigitated growth of pavement cells, with smooth shape. IPGA1 and AN had a genetic interaction in regulating pavement cell shape. Furthermore, IPGA1 genetically and physically interacted with the microtubule-severing enzyme KATANIN. We propose that the IPGA1-AN module regulates microtubule organisation and pavement cell shape.
Keyword ：
Arabidopsis Arabidopsis cortical microtubule cortical microtubule mechanical stress mechanical stress microtubule organisation microtubule organisation pavement cells pavement cells
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| GB/T 7714 | Chen, Binqing , Dang, Xie , Bai, Wenting et al. The IPGA1-ANGUSTIFOLIA module regulates microtubule organisation and pavement cell shape in Arabidopsis [J]. | NEW PHYTOLOGIST , 2022 , 236 (4) : 1310-1325 . |
| MLA | Chen, Binqing et al. "The IPGA1-ANGUSTIFOLIA module regulates microtubule organisation and pavement cell shape in Arabidopsis" . | NEW PHYTOLOGIST 236 . 4 (2022) : 1310-1325 . |
| APA | Chen, Binqing , Dang, Xie , Bai, Wenting , Liu, Min , Li, Ying , Zhu, Lilan et al. The IPGA1-ANGUSTIFOLIA module regulates microtubule organisation and pavement cell shape in Arabidopsis . | NEW PHYTOLOGIST , 2022 , 236 (4) , 1310-1325 . |
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Abstract ：
微管骨架通过导向纤维素微纤丝的合成来影响细胞壁的结构,在植物生长发育和细胞形态建成过程中发挥着重要作用。微管相关蛋白(microtubule-associated protein, MAP)参与调节微管蛋白亚基的组装、微管的动态稳定以及周质微管的排列方式。因此,对微管相关蛋白的研究有助于更好地理解微管骨架的生物学功能。本研究克隆了一个在拟南芥花瓣中高表达的假定微管相关蛋白PEMAP1 (Petal-expressed microtubuleassociated protein 1),并利用绿色荧光蛋白(GFP)和β-葡糖醛酸酶(GUS)作为报告蛋白对PEMAP1分别进行了亚细胞定位和组织表达模式分析。烟草瞬时表达实验结果表明,pSuper::PEMAP1-GFP能够与微管结合蛋白MBD-RFP共定位,拟南芥中内源启动子驱动的pPEMAP1::PEMAP1-GFP能够与微管株系m CherryTUA5共定位。GUS染色结果表明,PEMAP1启动子驱动的GUS拟南芥转基因株系在下胚轴、花萼和雌蕊的表达较强,在子叶、根部以及花瓣的表达则相对较弱。以上结果说明,PEMAP1很可能作为一个微管相关蛋白参与了拟南芥器官的生长发育与形态建成过程,相关遗传材料的获得为进一步研究PEMAP1的功能提供了工作基础。
Keyword ：
PEMAP1 PEMAP1 亚细胞定位 亚细胞定位 微管相关蛋白 微管相关蛋白 拟南芥(Arabidopsis thaliana) 拟南芥(Arabidopsis thaliana) 组织表达模式 组织表达模式
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| GB/T 7714 | 刘晓烽 , 林贺 , 陈斌卿 et al. 一个假定微管相关蛋白PEMAP1的克隆和亚细胞定位 [J]. | 分子植物育种 , 2021 , 19 (11) : 3598-3604 . |
| MLA | 刘晓烽 et al. "一个假定微管相关蛋白PEMAP1的克隆和亚细胞定位" . | 分子植物育种 19 . 11 (2021) : 3598-3604 . |
| APA | 刘晓烽 , 林贺 , 陈斌卿 , 党谢 , 林德书 . 一个假定微管相关蛋白PEMAP1的克隆和亚细胞定位 . | 分子植物育种 , 2021 , 19 (11) , 3598-3604 . |
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The microtubule cytoskeleton plays an important role in cell shape and plant development. During the past decades, the ability to use confocal microcopy to observe microtubules in living cells using fluorescent protein fusions has given plant scientists the opportunity to answer outstanding biological questions. Plants contain diverse epidermal cells with distinct morphologies and physiological functions. For example, flowering plants have specialized petal conical cells that likely facilitate functions such as providing grips for bee pollinators. Here, we summarize recent progress on live imaging of the microtubule cytoskeleton in conical cells. Firstly, we present a simple method for live-cell confocal imaging of conical cells, which is suitable for the quantification of the cell geometry. Secondly, we describe a method for observing microtubule organization in conical cells of Arabidopsis thaliana expressing greenfluorescent protein (GFP)-tagged alpha-tubulin 6 (GFP-TUA6). These live imaging approaches are likely to lead to rapid advances in our knowledge of the role of microtubules in conical cell shaping.
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| GB/T 7714 | Dang, Xie , Zhu, Lilan , Lin, Deshu . Live imaging of microtubules in petal conical cells [J]. | PLANT CELL BIOLOGY , 2020 , 160 : 253-261 . |
| MLA | Dang, Xie et al. "Live imaging of microtubules in petal conical cells" . | PLANT CELL BIOLOGY 160 (2020) : 253-261 . |
| APA | Dang, Xie , Zhu, Lilan , Lin, Deshu . Live imaging of microtubules in petal conical cells . | PLANT CELL BIOLOGY , 2020 , 160 , 253-261 . |
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The flowers of angiosperm species typically contain specialized conical cells. Although substantial progress has been achieved regarding the mechanisms underlying flower development, little is known about how petal cells achieve final conical shape. Here, we use 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) as a fluorescent pH indicator for analyzing the apoplastic pH of conical cells in Arabidopsis and show that normal conical cell expansion requires auxin signaling and apoplastic pH changes. By combining imaging analysis and genetic and pharmacological experiments, we demonstrate that apoplastic acidification and alkalization correlate with an increase and decrease in tip sharpening of conical cells, respectively. Initial expansion of conical cells is accompanied by decreased apoplastic pH, which is associated with increased auxin signaling. Decreased auxin levels, transport, or signaling abolishes cell wall acidification and causes reduced tip sharpening and heights of conical cells. These findings provide an insight into apoplastic pH regulation of conical cell expansion.
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| GB/T 7714 | Dang, Xie , Chen, Binqing , Liu, Fenglian et al. Auxin Signaling-Mediated Apoplastic pH Modification Functions in Petal Conical Cell Shaping [J]. | CELL REPORTS , 2020 , 30 (11) : 3904-, . |
| MLA | Dang, Xie et al. "Auxin Signaling-Mediated Apoplastic pH Modification Functions in Petal Conical Cell Shaping" . | CELL REPORTS 30 . 11 (2020) : 3904-, . |
| APA | Dang, Xie , Chen, Binqing , Liu, Fenglian , Ren, Huibo , Liu, Xin , Zhou, Jie et al. Auxin Signaling-Mediated Apoplastic pH Modification Functions in Petal Conical Cell Shaping . | CELL REPORTS , 2020 , 30 (11) , 3904-, . |
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Unlike animal cells, plant cells do not possess centrosomes that serve as microtubule organizing centers; how microtubule arrays are organized throughout plant morphogenesis remains poorly understood. We report here that Arabidopsis INCREASED PETAL GROWTH ANISOTROPY 1 (IPGA1), a previously uncharacterized microtubule-associated protein, regulates petal growth and shape by affecting cortical microtubule organization. Through a genetic screen, we showed that IPGA1 loss-of-function mutants displayed a phenotype of longer and narrower petals, as well as increased anisotropic cell expansion of the petal epidermis in the late phases of flower development. Map-based cloning studies revealed that IPGA1 encodes a previously uncharacterized protein that colocalizes with and directly binds to microtubules. IPGA1 plays a negative role in the organization of cortical microtubules into parallel arrays oriented perpendicular to the axis of cell elongation, with the ipga1-1 mutant displaying increased microtubule ordering in petal abaxial epidermal cells. The IPGA1 family is conserved among land plants and its homologs may have evolved to regulate microtubule organization. Taken together, our findings identify IPGA1 as a novel microtubule-associated protein and provide significant insights into IPGA1-mediated microtubule organization and petal growth anisotropy.
Keyword ：
Arabidopsis Arabidopsis cortical microtubule cortical microtubule growth anisotropy growth anisotropy INCREASED PETAL GROWTH ANISOTROPY 1 (IPGA1) INCREASED PETAL GROWTH ANISOTROPY 1 (IPGA1) microtubule-associated protein microtubule-associated protein petal petal
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| GB/T 7714 | Yang, Yanqiu , Chen, Binqinq , Dang, Xie et al. Arabidopsis IPGA1 is a microtubule-associated protein essential for cell expansion during petal morphogenesis [J]. | JOURNAL OF EXPERIMENTAL BOTANY , 2019 , 70 (19) : 5231-5243 . |
| MLA | Yang, Yanqiu et al. "Arabidopsis IPGA1 is a microtubule-associated protein essential for cell expansion during petal morphogenesis" . | JOURNAL OF EXPERIMENTAL BOTANY 70 . 19 (2019) : 5231-5243 . |
| APA | Yang, Yanqiu , Chen, Binqinq , Dang, Xie , Zhu, Lilan , Rao, Jinqiu , Ren, Huibo et al. Arabidopsis IPGA1 is a microtubule-associated protein essential for cell expansion during petal morphogenesis . | JOURNAL OF EXPERIMENTAL BOTANY , 2019 , 70 (19) , 5231-5243 . |
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Root hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of root hairs, contributions of membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a and YPT-INTERACTING PROTEIN 4b (YIP4a/b) contribute to activation and plasma membrane accumulation of Rho-of-plant (ROP) small GTPases during hair initiation, identifying YIP4a/b as central trafficking components in ROP-dependent root hair formation.
Keyword ：
Root hair Root hair ROP ROP Secretion Secretion Trans-Golgi network Trans-Golgi network YIP YIP
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| GB/T 7714 | Gendre, Delphine , Baral, Anirban , Dang, Xie et al. Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function [J]. | DEVELOPMENT , 2019 , 146 (5) . |
| MLA | Gendre, Delphine et al. "Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function" . | DEVELOPMENT 146 . 5 (2019) . |
| APA | Gendre, Delphine , Baral, Anirban , Dang, Xie , Esnay, Nicolas , Boutte, Yohann , Stanislas, Thomas et al. Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function . | DEVELOPMENT , 2019 , 146 (5) . |
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