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学者姓名：杨林蔚
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Abstract ：
Sea cucumber skin ulceration syndrome (SUS) is associated with a diverse range of bacterial and parasitic pathogens. Nonetheless, research efforts aimed at identifying novel pathogens and developing effective prevention and control strategies remain limited. In this study, we identified a strain of Photobacterium damselae and designated it as P. damselae sea cucumber xiapu (P. damselae SCXP), recognizing it as a new pathogenic bacterium associated with SUS in Apostichopus japonicus. We developed a detection method using a TaqMan probebased qPCR assay specific to P. damselae SCXP, which supports the rapid diagnosis of this pathogen. Additionally, artificial infection experiments confirmed that P. damselae SCXP facilitates the progression of SUS in Apostichopus japonicus. Drug sensitivity analysis revealed that the strain was highly sensitive to 16 antibiotics (e. g., novobiocin, ceftazidime, ceftriaxone) with inhibition zone diameters >= 20 mm, providing a reference for aquatic drug use. Furthermore, transcriptomic analyses were conducted on the coelomocytes of Apostichopus japonicus infected with P. damselae SCXP. The KEGG enrichment analysis identified distinct signaling pathways that were differentially expressed in the P. damselae SCXP-infected groups at 6, 12, and 24 h post-infection. At the 6-h mark, the focal adhesion pathway exhibited the highest number of differentially expressed genes (DEGs), followed by the PI3K-Akt, mTOR, and MAPK signaling pathways. At 12 h post-infection, the complement and coagulation cascades showed the highest number of DEGs, followed by phagosome-related pathways. By 24 h, the proteasome and lysosome pathways demonstrated an increased number of DEGs. Venn diagram analysis revealed that P. damselae SCXP infection suppressed the expression of several genes, including deoxyribonuclease-1 (DNase I) and acid-sensing ion channel 1-like protein (ASIC1), as validated by qRT-PCR. This study constitutes the first comprehensive investigation into the transcriptomic profile of Apostichopus japonicus coelomocytes in response to P. damselae SCXP infection. Future research endeavors should concentrate on elucidating the roles of DNase I and ASIC1 protein, as these elements may have significant implications for the development of targeted prevention and control strategies. Our study offers a theoretical foundation for the prevention and management of P. damselae-induced SUS in sea cucumbers.
Keyword ：
Photobacterium damselae Photobacterium damselae Sea cucumber Apostichopus japonicus Sea cucumber Apostichopus japonicus Skin ulceration syndrome Skin ulceration syndrome Transcriptomic Transcriptomic
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| GB/T 7714 | Yang, Linwei , Liu, Shuming , Lv, Hui et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome [J]. | AQUACULTURE , 2026 , 612 . |
| MLA | Yang, Linwei et al. "Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome" . | AQUACULTURE 612 (2026) . |
| APA | Yang, Linwei , Liu, Shuming , Lv, Hui , Wei, Haiyun , Wu, Tong , Lou, Bilian et al. Identification and transcriptomic analysis of a new pathogen, Photobacterium damselae SCXP, associated with sea cucumber skin ulceration syndrome . | AQUACULTURE , 2026 , 612 . |
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The DNA damage-regulated autophagy modulators have been identified as key activators of autophagy, an emerging mechanism to combat viral infections. In this study, we cloned and characterized the DNA damageregulated autophagy modulator 2 homolog from the shrimp Penaeus vannamei (designated as PvDRAM2) and investigated its potential roles during white spot syndrome virus (WSSV) infection. The predicted threedimensional structure of PvDRAM2 consists of six beta-fold regions, which exhibit conservation with similar structures in other species. Phylogenetic analysis revealed that the clusters of PvDRAM2 evolved alongside invertebrate DRAMs, showing a close relationship with DRAMs from Penaeus japonicus and Homarus americanus. Quantitative real-time PCR (qRT-PCR) analysis indicated that PvDRAM2 is ubiquitously expressed across various shrimp tissues, with significantly increased expression levels in hemocytes and intestines following WSSV challenge. RNA interference (RNAi)-mediated knockdown of PvDRAM2 notably elevated WSSV loads and reduced survival rates in shrimps subjected to WSSV challenge. Functionally, the overexpression of PvDRAM2 enhanced autophagy in High Five cells. Additionally, the application of autophagy activators demonstrated that activating autophagy could counteract the increased WSSV replication precipitated by the knockdown of PvDRAM2. Collectively, our findings suggest that PvDRAM2 may inhibit WSSV infection by activating autophagy.
Keyword ：
Autophagy Autophagy DRAM2 DRAM2 Penaeus vannamei Penaeus vannamei WSSV WSSV
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| GB/T 7714 | Huang, Xiyu , Zhao, Mingming , Huang, Jieyu et al. DRAM2 inhibits white spot syndrome virus infection via activating autophagy in Penaeus vannamei [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
| MLA | Huang, Xiyu et al. "DRAM2 inhibits white spot syndrome virus infection via activating autophagy in Penaeus vannamei" . | FISH & SHELLFISH IMMUNOLOGY 160 (2025) . |
| APA | Huang, Xiyu , Zhao, Mingming , Huang, Jieyu , Liu, Mingjian , Yang, Linwei , Wang, Chuanqi . DRAM2 inhibits white spot syndrome virus infection via activating autophagy in Penaeus vannamei . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 160 . |
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The spotted longbarbel catfish, Hemibagrus guttatus, a nationally protected Class II species in China, faces increasing threats from habitat degradation. Recently, the spotted longbarbel catfish has gained attention as a promising aquaculture species, not only for its premium flesh quality but also for its potential role in conservation through sustainable captive breeding programs. Ammonia nitrogen (ammonia-N) is a ubiquitous byproduct of intensive farming and serves as the primary environmental stressor confronting aquatic species. Elucidating the ammonia-N tolerance of spotted longbarbel catfish constitutes a critical prerequisite for its successful domestication, which is the aim of this study. We demonstrate that ammonia-N stress significantly decreases the survival rate of spotted longbarbel catfish and induces tissue damage, including gill lamella proliferation, hepatocyte blurring, and renal necrosis. Transcriptomic analysis revealed that ammonia-N stress promotes the expression of genes related to endoplasmic reticulum stress, heat-shock proteins, immune response, and apoptosis, while inhibiting antioxidant-related genes and Wnt-related genes. Enzymatic assays indicate that ammonia-N stress inhibits the activities of multiple antioxidant enzymes, including SOD, CAT, GSH, GSH-Px, and T-AOC. Microbiome analysis showed that ammonia-N stress altered the intestinal microbial community by increasing harmful bacteria (e.g., Vibrio and Aeromonas) and suppressing beneficial bacteria (e.g., Cetobacterium and Lactococcus). These findings highlight the comprehensive negative impacts of ammonia-N on the health of the spotted longbarbel catfish and provide a theoretical basis for optimizing aquaculture conditions to support the sustainable protection and domestication of the spotted longbarbel catfish.
Keyword ：
ammonia nitrogen ammonia nitrogen candidate species candidate species spotted longbarbel catfish spotted longbarbel catfish tolerance tolerance transcriptomic transcriptomic
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| GB/T 7714 | Guo, Song , Yang, Linwei , Xu, Xiaopeng . Assessing the Tolerance of Spotted Longbarbel Catfish as a Candidate Species for Aquaculture to Ammonia Nitrogen Exposure [J]. | ANIMALS , 2025 , 15 (14) . |
| MLA | Guo, Song et al. "Assessing the Tolerance of Spotted Longbarbel Catfish as a Candidate Species for Aquaculture to Ammonia Nitrogen Exposure" . | ANIMALS 15 . 14 (2025) . |
| APA | Guo, Song , Yang, Linwei , Xu, Xiaopeng . Assessing the Tolerance of Spotted Longbarbel Catfish as a Candidate Species for Aquaculture to Ammonia Nitrogen Exposure . | ANIMALS , 2025 , 15 (14) . |
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Research on finding alternative protein sources to replace fishmeal (FM) has become a central issue in the nutrition field. Extensive research has been carried out on the replacement of FM with soybean meal (SBM); however, little is known about the replacement of FM with enzyme-treated SBM (ESBM). In this study, five isolipidic and isonitrogenous diets were formulated by substituting FM with ESBM at the levels of 0% (FM, control diet), 5% (ESBM25), 10% (ESBM50), and 15% (ESBM75) which were fed to juvenile shrimp for 8 weeks. And we found that replacing FM with ESBM at 5%-10% levels in shrimp diets had no impact on shrimp growth performance and feed utilization. However, substituting 10% ESBM for FM in the shrimp diets promoted the expression of growth-related genes and maintained consistent intestinal microbiota compared to the control group. Replacing FM with 15% ESBM in the shrimp diets inhibited shrimp growth, suppressed mTOR gene expression, and promoted the proliferation of harmful intestinal bacteria. Furthermore, replacing FM with different ESBM did not affect the intestinal health of shrimp. Taken together, our research provides that replacing FM with 10% ESBM is feasible. These findings not only enrich our knowledge of FM proteinogen replacement but also provide a reference for the use of ESBM as a substitute for FM in commercial feeds for shrimp Penaeus vannamei as well as other shrimp species.
Keyword ：
enzyme-treated soybean meal enzyme-treated soybean meal fishmeal fishmeal Penaeus vannamei Penaeus vannamei replacement replacement
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| GB/T 7714 | Shao, Jianchun , Zheng, Qingyin , Chen, Zhengbang et al. Enzyme-Treated Soybean Meal Serves as an Effective Alternative to Fishmeal in the Diet of the Shrimp Penaeus vannamei [J]. | AQUACULTURE NUTRITION , 2025 , 2025 (1) . |
| MLA | Shao, Jianchun et al. "Enzyme-Treated Soybean Meal Serves as an Effective Alternative to Fishmeal in the Diet of the Shrimp Penaeus vannamei" . | AQUACULTURE NUTRITION 2025 . 1 (2025) . |
| APA | Shao, Jianchun , Zheng, Qingyin , Chen, Zhengbang , Zhu, Wenbo , Ren, Qiulei , Yuan, Kai et al. Enzyme-Treated Soybean Meal Serves as an Effective Alternative to Fishmeal in the Diet of the Shrimp Penaeus vannamei . | AQUACULTURE NUTRITION , 2025 , 2025 (1) . |
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C-type lectins (CTLs), a vital group of soluble pattern recognition receptors (PRRs), are essential to the shrimp's innate immune system. However, little is known about the CTLs composed of a C-type lectin domain (CTLD) and another domain. In this study, a novel C-type lectin containing a leucine-rich repeat (LRR) domain, designated LRRCTL, was identified in Penaeus vannamei and found to exhibit immune regulatory functions. First, LRRCTL expression was highest in the hepatopancreas. The mRNA expression levels of LRRCTL showed a significant upregulation under the different stimuli, including Vibrio parahaemolyticus, Staphylococcus aureus, Aspergillus niger, Lipopolysaccharide (LPS). Second, knockdown of the expression of LRRCTL increased the bacterial load of V. parahaemolyticus, whereas injection of rLRRCTL or r triangle LRR-LRRCTL recombinant proteins enhanced the survival rate of V. parahaemolyticus-infected shrimp. Additionally, the knockdown of LRRCTL expression reduced the mRNA level of multiple immune-related genes, encompassing both immune signaling components and antimicrobial peptide genes, suggesting an important role in immune regulation. Our findings reveal that LRRCTL is essential for regulating humoral immune responses, particularly antimicrobial peptide production, against V. parahaemolyticus infection. The findings provide a theoretical foundation for further investigation into the functional mechanisms of composite CTLs in shrimp innate immunity and potential molecular targets for developing strategies to prevent and control shrimp diseases.
Keyword ：
Immune regulatory Immune regulatory LRRCTL LRRCTL Penaeus vannamei Penaeus vannamei Vibrio parahaemolyticus Vibrio parahaemolyticus
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| GB/T 7714 | Weng, Kejia , Liu, Ruoyu , Xiong, Chengqi et al. A novel LRR domain-containing C-type lectin gene with immune regulatory functions protects Penaeus vannamei against Vibrio parahaemolyticus infection [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 165 . |
| MLA | Weng, Kejia et al. "A novel LRR domain-containing C-type lectin gene with immune regulatory functions protects Penaeus vannamei against Vibrio parahaemolyticus infection" . | FISH & SHELLFISH IMMUNOLOGY 165 (2025) . |
| APA | Weng, Kejia , Liu, Ruoyu , Xiong, Chengqi , Shi, Yuxin , Chen, Wenqian , Wang, Chuanqi et al. A novel LRR domain-containing C-type lectin gene with immune regulatory functions protects Penaeus vannamei against Vibrio parahaemolyticus infection . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 165 . |
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JAK/STAT signaling pathways are associated with the innate immune system and play important roles in mediating immune responses to virus infection. Domeless is a unique receptor involved in invertebrate JAK/ STAT pathway. In this study, a Domeless gene from Scylla paramamosain, named SpDOME, was cloned and characterized. The full length of SpDOME mRNA contains a 475 bp 5'untranslated region (UTR), an open reading frame (ORF) of 4188 bp and a 3 ' UTR of 195 bp. The SpDOME protein contains twenty-four amino acid signal peptide and six characteristic Domeless domains. In addition, the SpDOME showed 13 %-26 % identity and 44 %-60 % similarity to other DOME protein domains, respectively. The mRNA of SpDOME was expressed in all tissues, with higher expression in brain, intestine and ganglion, and lowest expression in hepatopancreas. Moreover, expression of SpDOME was significantly responsive to challenges by mud crab reovirus (MCRV), Poly (I:C) and LPS. Subcellular localization revealed that SpDOME were localized in the cytoplasm. SpDOME could activate SpSTAT to translocate from the cytoplasm to the nucleus, and significantly increase the transcription activity of the wsv069 promoter under stimulus of Poly(I:C) and LPS. Additionally, silencing of SpDOME in vivo increased the mortality of MCRV infected mud crab and the viral load in tissues and down-regulated the expression of multiple components of apoptosis and JAK-STAT pathways and almost all the examined immune effector genes. These findings suggest that SpDOME activates the JAK/STAT pathway and plays an important role in antiviral immunity in mud crab.
Keyword ：
Antiviral immune Antiviral immune DOME DOME JAK/STAT pathway JAK/STAT pathway MCRV MCRV Scylla paramamosain Scylla paramamosain
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| GB/T 7714 | Deng, Hengwei , Ke, Yetong , Weng, Shaoping et al. A cytokine receptor domeless restrains mud crab reovirus infection via JAK-STAT signaling pathway in mud crab (Scylla paramamosain) [J]. | FISH & SHELLFISH IMMUNOLOGY , 2025 , 163 . |
| MLA | Deng, Hengwei et al. "A cytokine receptor domeless restrains mud crab reovirus infection via JAK-STAT signaling pathway in mud crab (Scylla paramamosain)" . | FISH & SHELLFISH IMMUNOLOGY 163 (2025) . |
| APA | Deng, Hengwei , Ke, Yetong , Weng, Shaoping , Hu, Lei , Zhang, Wenfeng , Sun, Yujia et al. A cytokine receptor domeless restrains mud crab reovirus infection via JAK-STAT signaling pathway in mud crab (Scylla paramamosain) . | FISH & SHELLFISH IMMUNOLOGY , 2025 , 163 . |
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Keyword ：
Aeromonas hydrophila Aeromonas hydrophila antibacterial immune antibacterial immune aquatic animals aquatic animals epidemic diseases epidemic diseases innate immunity innate immunity
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| GB/T 7714 | Lu, Hu , Liao, Zhiwei , Sun, Jiejie et al. Editorial: Molecular innate immunity in aquatic animals and their response to epidemic diseases [J]. | FRONTIERS IN IMMUNOLOGY , 2025 , 16 . |
| MLA | Lu, Hu et al. "Editorial: Molecular innate immunity in aquatic animals and their response to epidemic diseases" . | FRONTIERS IN IMMUNOLOGY 16 (2025) . |
| APA | Lu, Hu , Liao, Zhiwei , Sun, Jiejie , Yang, Linwei , Deng, Hengwei . Editorial: Molecular innate immunity in aquatic animals and their response to epidemic diseases . | FRONTIERS IN IMMUNOLOGY , 2025 , 16 . |
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Although the immune function of the methyltransferase Wilm's tumor 1-associated protein (WTAP) has been well-studied in vertebrates, its role in the invertebrate immune system remains limited. In this study, we identified the WTAP gene in Penaeus vannamei and investigated its potential role in shrimp innate immunity. RT-qPCR analysis revealed that WTAP was broadly expressed in key immune tissues of the shrimp, including hemocytes, intestines, hepatopancreas, and gills. WTAP expression was upregulated in the hepatopancreas and gills during Vibrio parahaemolyticus infection. Although knockdown of WTAP did not significantly affect the survival rate of shrimp infected with V. parahaemolyticus, there was a non-significant trend toward decreased survival, along with markedly increased bacterial loads in gills and hepatopancreas. Knockdown of WTAP also led to a significant reduction in the expression levels of ALF1/3/5/6 and PEN2/3/4. Furthermore, WTAP expression was regulated by NF-kappa B Relish and repressed by the transcription factors STAT and Dorsal. Our results demonstrated that P. vannamei WTAP, regulated by NF-kappa B Relish, inhibited V. parahaemolyticus proliferation by modulating the expression of antimicrobial peptides (AMPs). These findings deepen our understanding of WTAP's role in crustacean innate immunity and provide a crucial foundation for future research on the relationship between m6A modification and immune regulation in invertebrates.
Keyword ：
Methyltransferase Methyltransferase Penaeus vannamei Penaeus vannamei Relish Relish Vibrio parahaemolyticus Vibrio parahaemolyticus WTAP WTAP
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| GB/T 7714 | Shi, Yuxin , Liu, Jingyang , Lin, Tongxin et al. Methyltransferase WTAP participates in shrimp defense against Vibrio parahaemolyticus infection [J]. | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY , 2025 , 172 . |
| MLA | Shi, Yuxin et al. "Methyltransferase WTAP participates in shrimp defense against Vibrio parahaemolyticus infection" . | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 172 (2025) . |
| APA | Shi, Yuxin , Liu, Jingyang , Lin, Tongxin , Lu, Wanli , Yang, Linwei , Huang, Siyou . Methyltransferase WTAP participates in shrimp defense against Vibrio parahaemolyticus infection . | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY , 2025 , 172 . |
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C-type lectins (CTLs), a class of carbohydrate-recognizing glycoproteins, play a vital role in immune response against bacterial infection. Vibrio parahaemolyticus is a major bacterial pathogen in shrimp, causing huge economic losses to shrimp farming. The role of the CTL family in anti-V. parahaemolyticus immunity requires further exploration. In this study, a novel CTL named Perlucin with immune regulatory functions was characterized from Litopenaeus vannamei. Perlucin was highly expressed in the muscle and hepatopancreas of healthy L. vannamei. The mRNA levels of Perlucin were significantly upregulated after LPS stimulation, and V . parahaemolyticus, Staphylococcus aureus and Aspergillus niger infections. Silencing of Perlucin by injection of specific dsRNA decreased the survival rate of V. parahaemolyticus-infected shrimp and increased the bacterial load of V . parahaemolyticus in tissues, while injection of recombinant Perlucin protein had the opposite effect. Moreover, silencing of Perlucin significantly affected the expression of multiple immune-related genes, including immune signaling components and downstream effector genes, suggesting that Perlucin is involved in immune regulation. This suggests that perlucin plays a crucial role in regulating humoral immune response against V. parahaemolyticus infection in shrimp.
Keyword ：
Immune regulatory Immune regulatory Litopenaeus vannamei Litopenaeus vannamei Perlucin Perlucin Vibrio parahaemolyticus Vibrio parahaemolyticus
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| GB/T 7714 | Wang, Zi-ang , Yang, Linwei , Zhao, Zexu et al. A novel perlucin with immune regulatory functions protects Litopenaeus vannamei against Vibrio parahaemolyticus infection [J]. | FISH & SHELLFISH IMMUNOLOGY , 2024 , 155 . |
| MLA | Wang, Zi-ang et al. "A novel perlucin with immune regulatory functions protects Litopenaeus vannamei against Vibrio parahaemolyticus infection" . | FISH & SHELLFISH IMMUNOLOGY 155 (2024) . |
| APA | Wang, Zi-ang , Yang, Linwei , Zhao, Zexu , Weng, Shaoping , He, Jianguo , Xu, Xiaopeng . A novel perlucin with immune regulatory functions protects Litopenaeus vannamei against Vibrio parahaemolyticus infection . | FISH & SHELLFISH IMMUNOLOGY , 2024 , 155 . |
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