Query：
学者姓名：王子瑄
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
Enhancing the field persistence of Bacillus thuringiensis (Bt) biopesticides is crucial for efficient pest management. This study presents the first systematic investigation into the mechanisms by which all 20 L-amino acids influence Bt biofilm formation, ultraviolet (UV) resistance, and insecticidal activity. The results demonstrate that amino acids functioned as interfacial modulators: non-polar hydrophobic types (e.g., L-leucine) significantly increased biofilm biomass in wettable powder through hydrophobic adhesion, whereas basic types (e.g., Larginine, L-lysine) enhanced the stability of aqueous suspension concentrate via electrostatic stabilization. Through statistical optimization (Plackett-Burman design and response surface methodology), a synergistic formulation composed of L-arginine, L-lysine, and L-leucine was identified. This formulation increased the biofilm yield of the commercial Kernel 32,000 IU/mg wettable powder by 3.8-fold and improved survival rate by approximately 50 % after 4 h of UV irradiation. At the physio-toxicological level, this amino acid combination reduced the LC50 against second-instar Plutella xylostella larvae by 8.12-fold. Transmission electron microscopy revealed complete microvilli shedding and mitochondrial cristae breakage in the treated midgut, indicating that the synergistic mechanism stems from the disruption of the midgut epithelial barrier. This study provides the first direct link between amino-acid-mediated interfacial interactions and midgut pathological damage, offering molecular-physiological evidence for next-generation, low-dose, highly stable Bt formulation with manageable resistance risks.
Keyword ：
Amino acid synergism Amino acid synergism Bacillus thuringiensis (Bt) Bacillus thuringiensis (Bt) Biofilm formation Biofilm formation Biopesticide optimization Biopesticide optimization Midgut pathophysiology Midgut pathophysiology UV resistance UV resistance
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wang, Zixuan , Zhang, Yimeng , Chen, Junjun et al. L-amino acids modulate biofilm formation, UV resistance, and insecticidal activity in Bacillus thuringiensis formulations: Mechanisms and optimization [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2026 , 216 . |
| MLA | Wang, Zixuan et al. "L-amino acids modulate biofilm formation, UV resistance, and insecticidal activity in Bacillus thuringiensis formulations: Mechanisms and optimization" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 216 (2026) . |
| APA | Wang, Zixuan , Zhang, Yimeng , Chen, Junjun , Liu, Qingqing , Luo, Xueqin , Xu, Qiuting et al. L-amino acids modulate biofilm formation, UV resistance, and insecticidal activity in Bacillus thuringiensis formulations: Mechanisms and optimization . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2026 , 216 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Exopolysaccharides (EPS) produced by Bacillus species display various biological activities and characteristics such as anti-oxidant, immunomodulatory, anti-bacterial, and bioadhesive effects. These attributes confer Bacillus species broad potential applications in diverse fields such as food, medicine, environment, and agriculture. Moreover, Bacillus-derived EPS are easier to produce and yield higher quantities than plant-derived polysaccharides. Despite these advantages, Bacillus-derived EPS still encounter numerous obstacles in industrial production and commercial applications, including elevated costs, the absence of mature fermentation tank production procedures, and the lack of systematic in vivo and in vitro activity and metabolic evaluation. Therefore, it is essential to gain insight into the current status of structure, production, and applications of Bacillus-derived EPS for facilitating their future broader application. This paper provides a comprehensive overview of the current research on the production, separation, characteristics and applications of these related biological products. Furthermore, this paper summarizes the current challenges impeding industrial production of Bacillus-derived EPS, along with potential solutions, and their prospective applications in enhancing the attributes of beneficial biofilms, laying a solid scientific foundation for the applications of Bacillus-derived EPS in industry and agriculture.
Keyword ：
Applications Applications Bacillus Bacillus Beneficial biofilms Beneficial biofilms Characteristics Characteristics Exopolysaccharides Exopolysaccharides Production Production
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Yang, Xiaolong , Mao, Yufei , Chen, Lan et al. Structural characteristics, biotechnological production and applications of exopolysaccharides from Bacillus sp.: A comprehensive review [J]. | CARBOHYDRATE POLYMERS , 2025 , 355 . |
| MLA | Yang, Xiaolong et al. "Structural characteristics, biotechnological production and applications of exopolysaccharides from Bacillus sp.: A comprehensive review" . | CARBOHYDRATE POLYMERS 355 (2025) . |
| APA | Yang, Xiaolong , Mao, Yufei , Chen, Lan , Guan, Xiong , Wang, Zixuan , Huang, Tianpei . Structural characteristics, biotechnological production and applications of exopolysaccharides from Bacillus sp.: A comprehensive review . | CARBOHYDRATE POLYMERS , 2025 , 355 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明涉及一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法。所述方法具体为：向苏云金芽胞杆菌制剂中添加有机酸，从而提升苏云金芽胞杆菌制剂的生物活性；所述苏云金芽胞杆菌制剂为苏云金芽胞杆菌可湿性粉剂；所述有机酸选自棕榈酸、硬脂酸、花生酸、乙酰水杨酸、油酸中的任意一种或多种；所述生物活性包括生物被膜形成能力、抗紫外线能力、杀虫活性中的任意一种或多种。通过本发明的方法，能够显著提升苏云金芽胞杆菌制剂在农业中的应用效果，具有较高的实际应用价值。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 黄天培 , 赵梵 , 毛雨飞 et al. 一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法 : CN202411726650.4[P]. | 2024-11-28 . |
| MLA | 黄天培 et al. "一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法" : CN202411726650.4. | 2024-11-28 . |
| APA | 黄天培 , 赵梵 , 毛雨飞 , 王子瑄 , 苏泓昕 , 梅智峰 et al. 一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法 : CN202411726650.4. | 2024-11-28 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Phyllotreta striolata is a major pest of Brassica chinensis, with chemical pesticides being the primary method for pest control. However, their negative impact on crop growth and environmental risks have prompted the search for low-toxicity, environmentally friendly alternatives. This study systematically evaluates the control effectiveness of two commonly used chemical pesticides, Zu Jia (R) and Jie Tiao (R), along with their impact on the growth characteristics of B. chinensis and compares them to the biopesticide Bacillus thuringiensis (Bt) strain G033A. The control efficacy was assessed by measuring the insect population reduction rate and plant physiological indicators (weight, plant height, chlorophyll content, and root length). The differences between biopesticide and the two chemical pesticide treatments were analyzed. The results indicated that both Bt strain G033A and the chemical pesticides significantly controlled P. striolata. However, chemical pesticides (Zu Jia (R) and Jie Tiao (R)) significantly reduced the chlorophyll content, plant height, and weight of B. chinensis (p < 0.05), whereas Bt strain G033A had no significant adverse effects on these plant traits (p > 0.05). The colonization characteristics of the Bt strain suggest potential for sustained pest control, while chemical pesticides exhibited rapid pest suppression effects. The findings highlight a novel IPM strategy that integrates the long-lasting efficacy of Bt biopesticides with the rapid action of chemical pesticides, offering a more sustainable and balanced approach to P. striolata management in B. chinensis cultivation.
Keyword ：
biopesticides biopesticides Brassica chinensis Brassica chinensis chemical pesticides chemical pesticides integrated pest management integrated pest management Phyllotreta striolata Phyllotreta striolata
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lin, Fuyong , Muhammad, Musa Hassan , Mao, Yufei et al. Comparative Control of Phyllotreta striolata: Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide [J]. | INSECTS , 2025 , 16 (6) . |
| MLA | Lin, Fuyong et al. "Comparative Control of Phyllotreta striolata: Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide" . | INSECTS 16 . 6 (2025) . |
| APA | Lin, Fuyong , Muhammad, Musa Hassan , Mao, Yufei , Zhao, Fan , Wang, Zixuan , Hong, Yongcong et al. Comparative Control of Phyllotreta striolata: Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide . | INSECTS , 2025 , 16 (6) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The rational design of gene delivery systems using peptides and their derivatives has gained great interest in improving treatment efficacy in recent years. However, peptide-based systems face challenges such as instability and low bioavailability, which limit their clinical application. To maximize therapeutic effects while minimizing adverse effects, peptide drug delivery systems must efficiently target lesion sites, traverse cellular barriers, and release drugs effectively. This review will discuss the historical progress, current development status, and future prospects of peptide-based gene delivery systems. Moreover, this review will focus on the transportation process and biological barriers encountered by peptide carriers in gene delivery, discussing the common structures and design principles of peptide carriers, with an emphasis on the unique role of peptides in improving or controlling drug delivery performance in vivo, as well as the customization of peptide carriers for specific applications in gene therapy.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wang, Zixuan , Zhou, Jialin , Su, Rongxin et al. Design of peptide-based gene delivery systems to overcome biological barriers [J]. | JOURNAL OF MATERIALS CHEMISTRY B , 2025 , 13 (40) : 12738-12764 . |
| MLA | Wang, Zixuan et al. "Design of peptide-based gene delivery systems to overcome biological barriers" . | JOURNAL OF MATERIALS CHEMISTRY B 13 . 40 (2025) : 12738-12764 . |
| APA | Wang, Zixuan , Zhou, Jialin , Su, Rongxin , Qi, Wei , Wang, Yuefei . Design of peptide-based gene delivery systems to overcome biological barriers . | JOURNAL OF MATERIALS CHEMISTRY B , 2025 , 13 (40) , 12738-12764 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Incorporating d-amino acids into peptides can influence the intermolecular interactions of peptides, thus determining the morphology and functionality of self-assembled supramolecular structures. Based on this, we propose a modular chirality regulation strategy and designed four chiral peptides by adjusting the chirality of different functional modules. The chirality can control the coassembly of peptides and nucleic acids into virus-like vesicles with controlled diameters and enzyme-responsiveness. Compared with homochiral peptides, the heterochiral peptides with chirality inversion in their hydrophobic domain transformed into more hydrophobic assemblies in response to the highly expressed enzyme matrix metalloproteinase 7 (MMP-7) in cancer cells and showed higher endosomal membrane disruption activity. Moreover, the heterochiral peptides exhibit high efficiency and selectivity in delivering siRNA gene drugs and inhibiting cancer cell growth, achieving a mortality rate of 95% in cancer cells. These results provide a promising strategy for designing peptide-based nucleic acid delivery systems through chiral modulation.
Keyword ：
anticancer anticancer gene delivery gene delivery heterochirality heterochirality peptide peptide self-assembly self-assembly
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wang, Zixuan , Yue, Lei , Min, Jiwei et al. Control the Gene Delivery and Anticancer Efficacy of Peptides through Chiral Modulation [J]. | NANO LETTERS , 2025 , 25 (7) : 2693-2701 . |
| MLA | Wang, Zixuan et al. "Control the Gene Delivery and Anticancer Efficacy of Peptides through Chiral Modulation" . | NANO LETTERS 25 . 7 (2025) : 2693-2701 . |
| APA | Wang, Zixuan , Yue, Lei , Min, Jiwei , Liu, Huiye , Zhang, Yexi , Du, Yaohui et al. Control the Gene Delivery and Anticancer Efficacy of Peptides through Chiral Modulation . | NANO LETTERS , 2025 , 25 (7) , 2693-2701 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
This study investigated the effects of fertilizers on the biofilm formation, ultraviolet (UV) resistance, and insecticidal activity of Bacillus thuringiensis (Bt). Bacillus thuringiensis, a widely used microbial pesticide, has a minimal environmental impact and is highly effective against specific pests but is susceptible to environmental factors in field applications. Bacterial biofilms provide protection for Bt, enhancing its survival and functionality in the environment. However, the mechanisms by which fertilizers regulate the characteristics of microbial pesticides and enhance biofilm formation are not well understood. This study evaluated the effects of six fertilizers on the bacterial biofilm formation, the UV resistance, and the insecticidal activities of Bt wettable powders. The results demonstrated that fertilizers significantly enhanced the performance of three Bt preparations (Lv'an, Kang'xin, and Lu'kang). A compound fertilizer with 8.346 g/L of KCl, 2.751 g/L of ZnSO47H2O, and 25.681 mu L/mL of humic acid was identified by response surface optimization, achieving the maximum BBF formation with OD595 value of 2.738. Furthermore, KH2PO4, HA, and ZnSO47H2O notably improved the survivability of Bt preparations under prolonged UV exposure, with the compound fertilizer providing the greatest protection. What's more, fertilizers reduced the LC50 values of all Bt preparations, with the compound fertilizer decreasing the LC50 of the Lv'an Bt wettable powder to 0.139 g/L, a 3.12-fold increase in efficacy. This study demonstrated that fertilizers significantly enhance the UV resistance and insecticidal activity of Bt wettable powders by promoting bacterial biofilm formation. Herein, this study provides new strategies and theoretical support for Bt applications in modern sustainable agriculture.
Keyword ：
Bacillus thuringiensis Bacillus thuringiensis bacterial biofilm bacterial biofilm biocontrol biocontrol fertilizers fertilizers insecticidal activity insecticidal activity wettable powders wettable powders
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhao, Fan , Mao, Yufei , Yang, Jiahong et al. Enhancing Bacillus thuringiensis Performance: Fertilizer-Driven Improvements in Biofilm Formation, UV Protection, and Pest Control Efficacy [J]. | MICROORGANISMS , 2025 , 13 (3) . |
| MLA | Zhao, Fan et al. "Enhancing Bacillus thuringiensis Performance: Fertilizer-Driven Improvements in Biofilm Formation, UV Protection, and Pest Control Efficacy" . | MICROORGANISMS 13 . 3 (2025) . |
| APA | Zhao, Fan , Mao, Yufei , Yang, Jiahong , Yang, Sheng , Guan, Xiong , Wang, Zixuan et al. Enhancing Bacillus thuringiensis Performance: Fertilizer-Driven Improvements in Biofilm Formation, UV Protection, and Pest Control Efficacy . | MICROORGANISMS , 2025 , 13 (3) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Over 200 weed species-predominantly from the Poaceae and Asteraceae families-infest tea plantations. These weeds compete aggressively with tea plants for essential resources (light, water, and nutrients), serve as reservoirs for pathogens and pests, and bioaccumulate phytotoxic compounds (e.g., pyrrolizidine alkaloids and heavy metals), jeopardizing agroecosystem health. Although chemical herbicides are currently the most effective and economical method, their long-term overuse poses ecological risks and threatens agricultural sustainability, highlighting an urgent need for sustainable alternatives. Although chemical herbicides remain the most effective and economical method, their long-term overuse causes ecological risks and challenges agricultural sustainability. This review synthesizes current knowledge on sustainable weed management in tea plantations by evaluating the strengths and limitations of chemical and microbial herbicides. It further advances the novel concepts of chemical-microbial synergy and smart application triangulation-integrated strategies not explicitly addressed in previous reviews-that provide new directions for developing next-generation sustainable weed control. This review systematically examines three critical aspects of weed management in tea plantations: (1) characterizing weed biodiversity and assessing multidimensional threats through taxonomic profiling of invasive species, with particular focus on their cascading impacts on tea agroecosystems including resource competition, disease transmission, and soil toxicity; (2) evaluating the mechanisms and trade-offs of current herbicide approaches through comparative analysis of chemical herbicides (noting their efficiency but ecological concerns) and microbial herbicides (considering their environmental benefits but field performance variability); (3) exploring sustainable optimization strategies by investigating innovative approaches such as precision herbicide formulations (nano-formulations, smart delivery systems) and integrated weed management solutions (microbiome engineering, epigenome editing).
Keyword ：
Chemical herbicides Chemical herbicides Herbicide performance Herbicide performance Microbial herbicides Microbial herbicides Multidimensional hazards Multidimensional hazards Sustainable weed management Sustainable weed management Tea plantation weeds Tea plantation weeds
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen Lan , Yang Xiaolong , Su Zhongzeng et al. Sustainable tea plantations: Harnessing chemical-microbial synergy and smart application triangulation for targeted weed control. [J]. | Journal of advanced research , 2025 . |
| MLA | Chen Lan et al. "Sustainable tea plantations: Harnessing chemical-microbial synergy and smart application triangulation for targeted weed control." . | Journal of advanced research (2025) . |
| APA | Chen Lan , Yang Xiaolong , Su Zhongzeng , Guan Xiong , Wang Zixuan , Huang Tianpei . Sustainable tea plantations: Harnessing chemical-microbial synergy and smart application triangulation for targeted weed control. . | Journal of advanced research , 2025 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
