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学者姓名：张文盼
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Abstract ：
Sugarcane (Saccharum hybrids spp.) is the most important sugar crop that accounts for similar to 75% of the world's sugar production. Recently, a whole-genome sequencing project was launched on the wild species S. spontaneum. To obtain information on the DNA composition of the repeat-enriched region of the centromere, we conducted a genome-wide analysis of the DNA sequences associated with CenH3 (a mutant of histone H3 located in eukaryote centromeres) using chromatin immunoprecipitation followed by sequencing (ChIP-seq) method. We demonstrate that the centromeres contain mainly SCEN-like single satellite repeat (Ss1) and several Ty3/gypsy retrotransposon-related repeats (Ss166, Ss51, and Ss68). Ss1 dominates in the centromeric regions and spans up to 500 kb. In contrast, the Ty3/gypsy retrotransposon-related repeats are either clustered spanning over a short range, or dispersed in the centromere regions. Interestingly, Ss1 exhibits a chromosome-specific enrichment in the wild species S. spontaneum and S. robustum, but not in the domesticated species S. officinarum and modern sugarcane cultivars. This finding suggests an autopolyploid genome identity of S. spontaneum with a high level of homology among its eight sub-genomes. We also conducted a genome-wide survey of the repetitive DNAs in S. spontaneum following a similarity-based sequence clustering strategy. These results provide insight into the composition of sugarcane genome as well as the genome assembly of S. spontaneum.
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| GB/T 7714 | Zhang, Wenpan , Zuo, Sheng , Li, Zhanjie et al. Isolation and characterization of centromeric repetitive DNA sequences in Saccharum spontaneum [J]. | SCIENTIFIC REPORTS , 2017 , 7 . |
| MLA | Zhang, Wenpan et al. "Isolation and characterization of centromeric repetitive DNA sequences in Saccharum spontaneum" . | SCIENTIFIC REPORTS 7 (2017) . |
| APA | Zhang, Wenpan , Zuo, Sheng , Li, Zhanjie , Meng, Zhuang , Han, Jinlei , Song, Junqi et al. Isolation and characterization of centromeric repetitive DNA sequences in Saccharum spontaneum . | SCIENTIFIC REPORTS , 2017 , 7 . |
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Abstract ：
甘蔗是非整倍性异源多倍体作物,遗传背景高度复杂,其细胞生物学研究难度大。近年分子标记技术以及原位杂交技术等快速发展使甘蔗细胞学研究也取得明显进展。本文主要围绕甘蔗核型、染色体行为、原位杂交技术在甘蔗上的应用展开综述,以期为高倍体植物细胞学研究提供参考。
Keyword ：
原位杂交技术 原位杂交技术 多倍体 多倍体 染色体 染色体 甘蔗 甘蔗
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| GB/T 7714 | 沈姣 , 王凯 , 张文盼 et al. 甘蔗染色体研究近况 [J]. | 热带作物学报 , 2016 , 37 (07) : 1430-1436 . |
| MLA | 沈姣 et al. "甘蔗染色体研究近况" . | 热带作物学报 37 . 07 (2016) : 1430-1436 . |
| APA | 沈姣 , 王凯 , 张文盼 , 董广蕊 , 石岩 , 张积森 . 甘蔗染色体研究近况 . | 热带作物学报 , 2016 , 37 (07) , 1430-1436 . |
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本发明提供了雷蒙德氏棉功能着丝粒序列及其相应的分子标记。我们利用染色质免疫共沉淀与高通量测序技术相结合(ChIP-Seq)的方法得到了雷蒙德氏棉功能着丝粒区域的四条DNA序列。根据该序列分别设计引物，用于雷蒙德氏棉有丝分裂中期荧光原位杂交(FISH)。结果显示，这四条序列均在全部染色体的着丝粒区域产生集中且清晰明亮的信号。本发明的着丝粒序列可直接用于功能着丝粒的定位及相关序列的鉴定；所开发的标记可用于棉花遗传图谱着丝粒位置的遗传定位，为功能基因定位及连锁关系提供更加准确的信息，也能为雷蒙德氏棉全基因组测序的序列拼接提供借鉴和帮助，使得其物理图谱信息更加全面。
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| GB/T 7714 | 王凯 , 韩金磊 , 张文盼 et al. 雷蒙德氏棉功能着丝粒序列及其分子标记 : CN201510225766.4[P]. | 2015-05-06 . |
| MLA | 王凯 et al. "雷蒙德氏棉功能着丝粒序列及其分子标记" : CN201510225766.4. | 2015-05-06 . |
| APA | 王凯 , 韩金磊 , 张文盼 , 俞丽英 , 单文波 , 林爱婷 . 雷蒙德氏棉功能着丝粒序列及其分子标记 : CN201510225766.4. | 2015-05-06 . |
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